Article
Food Science & Technology
Franc Cus, Helena Basa Cesnik, Spela Velikonja Bolta
Summary: This study aimed to compare pesticide residues, copper, and biogenic amines concentrations in conventional and organic wines. Results showed lower pesticide residues in organic wines and higher copper levels in conventional wines.
Article
Food Science & Technology
Jesus Lopez-Santiago, Ana Isabel Garcia Garcia, Maria Teresa Gomez-Villarino
Summary: This study analyzes the performance of Prerequisites Programs (PPRs) and Hazard Analysis Critical Control Point (HACCP) in wineries in the Protected Denomination of Origin Vinos de Madrid. The study reveals that the control of metals in grapes and the control of fungicides or pesticides in the harvest reception have the worst performance in terms of critical control points (CCPs). While most wineries have implemented PPRs and HACCP, there are issues with the practical implementation of corrective action procedures and verification procedures.
Article
Chemistry, Multidisciplinary
Yang Liu, Liqiang Liu, Xinxin Xu, Chuanlai Xu, Liguang Xu
Summary: In this study, a novel hapten was designed to produce a highly sensitive and specific monoclonal antibody for the detection of flutriafol (FTF) residues in crops. Additionally, a gold nanoparticle-based lateral flow immunoassay (LFIA) was developed for the quantitative detection of FTF in rice, tomatoes, and grapes, with a visual limit of detection of 0.5 mg kg(-1), 0.2 mg kg(-1) and 0.5 mg kg(-1), respectively. The recoveries in spiked samples ranged from 90.2% to 110.1%, indicating the reliability of this LFIA as an on-site detection method.
MATERIALS CHEMISTRY FRONTIERS
(2023)
Article
Chemistry, Analytical
Eric Ceballos-Alcantarilla, Antonio Abad-Fuentes, Consuelo Agullo, Antonio Abad-Somovilla, Josep Mercader
Summary: Immunoassays are widely used for rapid contaminant analysis in various samples, with the characterization of antibody-antigen interaction being crucial for developing sensitive tests. Competitive strategies are often employed for low molecular weight compounds to estimate apparent affinity values. In this study, monoclonal antibodies to penthiopyrad were successfully raised, with apparent equilibrium constants assessed using competitive enzyme-linked immunosorbent assays. The study showed close agreement between the obtained KD values and those calculated from surface plasmon resonance evaluation, and the developed immunoassay demonstrated good correlation with LC-MS/MS results.
Article
Food Science & Technology
Shaowen Liu, Aijuan Bai, Le Song, Nan Zou, Yongtao Han, Li Zhou, Chuanshan Yu, Changjun Li, Canping Pan
Summary: A convenient and fast multi-residue method has been developed for the efficient identification and quantification of 72 pesticides in red and white grape wines. The method achieved consistent recoveries and reduced sample matrix compounds. It was successfully used to detect pesticide residues in commercial grape wines.
Article
Environmental Sciences
Zhiguang Hou, Xingang Hou, Liping Wei, Zhanwen Cao, Zhou Lu, Hanju Liu, Zhongbin Lu
Summary: This study established an analysis method for mandipropamid in ginseng and investigated its degradation kinetics and residue content. The results showed that mandipropamid at the recommended dose does not pose unacceptable risks to humans.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Food Science & Technology
Wanyao Song, Mengyu Luo, Huaming Li, Jiaxu Xiao, Xiuping He, Jixiang Liang, Dapeng Peng
Summary: In this study, an ic-ELISA method was developed using a new generation metabolite (DMEQ)-based monoclonal antibody (mAb) for the rapid detection of Qx residues in food. The method exhibited high sensitivity and a wide linear range, and showed good cross-reactivity with multiple DQx. Furthermore, the method demonstrated good correlation with LC-MS/MS results in animal-derived foods, indicating its potential for rapid screening of Qx residues.
Article
Food Science & Technology
Noemi Sofia Rabitti, Camilla Cattaneo, Marta Appiani, Cristina Proserpio, Monica Laureati
Summary: The Rate-All-That-Apply method (RATA) with semi-trained judges was used to characterize a large and heterogeneous Italian wine sample. The judges showed high repeatability and good panel reliability. The multi-factor analysis revealed the discriminatory ability of the panel, with red wines described by bitterness, astringency, body, alcohol, and specific olfactory stimuli, while white wines were described by saltiness, sourness, and citrus, tropical fruits, and white flowers odors. The RATA method is a suitable and reliable alternative to gather information about the sensory perception of wines, even with limited availability of consumer panels.
Review
Agriculture, Multidisciplinary
Lu Yang, Fan Wei, Jing-Min Liu, Shuo Wang
Summary: This review discusses the advances in agro-food safety inspection techniques utilizing new hybrid micro/nanoentities, such as molecular imprinting polymers integrated with quantum dots and upconversion luminescent nanoparticles. The integration of various disciplines like material science, nanotechnology, and analytical methodology shows promise in addressing emerging challenges in the field of agro-food research.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Review
Food Science & Technology
Zhuoao Jiang, Yu Zhuang, Shentian Guo, A. S. M. Muhtasim Fuad Sohan, Binfeng Yin
Summary: Food safety is a significant issue worldwide, and pesticide residues in food pose a serious risk to human health. Traditional methods for analyzing pesticide residues have limitations, such as being time-consuming and costly. Therefore, there is a need to develop rapid, effective, and sensitive equipment for analyzing pesticide residues in food. This paper summarizes the application of microfluidic techniques to detect pesticide residues in food, as well as the development of microfluidics in combination with 3D printing technology and nanomaterials.
Article
Food Science & Technology
Wenzhi Tang, Yangchun Qi, Zhonghong Li
Summary: This study developed a portable, low-cost, and user-friendly colorimetric instrument for ELISA and aflatoxin B-1 detection. The instrument demonstrated stability, accuracy, quantitative detection capability, and insensitivity to ambient light changes, making it user-friendly. Measurements showed accurate results comparable to professional equipment, presenting attractive potential for rapid AFB(1) detection.
Article
Biochemistry & Molecular Biology
Anna Przybylska, Agnieszka Chrustek, Beata Sperkowska, Marcin Koba, Dorota Olszewska-Slonina
Summary: Naturally gluten-free foods and processed foods without information about potential gluten presence pose a hypothetical threat to people with food allergies and celiac disease. This research analyzed certified gluten-free and naturally gluten-free products in terms of their gluten protein content. The results showed that most products were safe, but some had higher gluten levels. Additionally, commercial beers containing barley malt were found to be mostly unsuitable for a strict gluten-free diet. Therefore, there is a need to increase testing frequency by food manufacturers to provide more products suitable for those on a gluten-free diet.
Article
Food Science & Technology
Alessandra Aparecida Zinato Rodrigues, Maria Eliana Lopes Ribeiro de Queiroz, Leda Rita D'Antonino Faroni, Lucas Henrique Figueiredo Prates, Antonio Augusto Neves, Andre Fernando de Oliveira, Jessika Faeda de Freitas, Fernanda Fernandes Heleno, Laercio Zambolim
Summary: This study investigated the effectiveness of different methods in removing pesticide residues from bell peppers, finding that water continuously bubbled with ozone and low-concentration traditional washing solutions can effectively remove pesticide residues while preserving the quality of the fruit. Sodium bicarbonate in traditional washing solutions showed good efficiency in removing pesticide residues but affected the color quality of the fruit.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Food Science & Technology
Zuzana Miskova, Eva Lorencova, Richardos Nikolaos Salek, Tereza Kolackova, Ludmila Travnikova, Anita Rejdlova, Leona Bunkova, Frantisek Bunka
Summary: The aim of this study was to monitor and evaluate the content of eight biogenic amines (BAs) in 232 samples of wines produced in the Central European region (Zone B). PUT, HIS, TYM, and PEA were the most frequently detected BAs in all tested wine samples. The BA content in red wines was generally higher than in rose and white wines, with 9% of the red wine samples having histamine concentrations above the recommended limit of 10 mg/L.
Article
Chemistry, Multidisciplinary
Erwin G. Abucayon, Connor Whalen, Oscar B. Torres, Alexander J. Duval, Agnieszka Sulima, Joshua F. G. Antoline, Therese Oertel, Rodell C. Barrientos, Arthur E. Jacobson, Kenner C. Rice, Gary R. Matyas
Summary: We proposed an equilibrium dialysis (ED)-based approach coupled with fluorimetry (ED-fluorimetry) to measure the antibody binding-site concentration to the ligand in an aqueous environment. The measured binding-site concentrations by ED-fluorimetry are in agreement with those determined by a more established method. Importantly, the measured concentrations were not affected by the sample matrix, indicating the validity of this method for determining the binding-site concentration of polyclonal antibodies in serum samples. This simple and rapid approach has the potential to reliably perform quantitative antibody binding-site screening in serum and other complex biological fluids.
Article
Biochemical Research Methods
Asuncion Morales-Noe, Francesc A. Esteve-Turrillas, Sergio Armenta
Summary: Synthetic cannabinoids are a major group of new psychoactive substances with high potency effects, and their metabolism is usually entirely unknown. This study evaluated the metabolism of five third generation synthetic cannabinoids using zebrafish larvae as a model and proposed metabolic pathways for each compound. Zebrafish larvae, with genetic similarity to humans, are a useful tool for identifying the metabolic pathways of psychoactive substances.
DRUG TESTING AND ANALYSIS
(2022)
Article
Food Science & Technology
Antonio Abad-Fuentes, Consuelo Agullo, Daniel Lopez-Puertollano, Ismael Navarro-Fuertes, Antonio Abad-Somovilla, Josep Vicent Mercader
Summary: Appropriate hapten design and synthesis are critical for generating high-performance immunoreagents and developing sensitive and selective immunoanalytical methods. In this study, novel haptens of zearalenone were designed and evaluated for their structure-activity relationship. The results showed that modifying the hapten structure can affect antibody affinity and cross-reactivity, and changing the competing antigen structure can modify the immunoassay selectivity. These findings suggest that the immunochemical methods for zearalenone rapid analysis can still be improved in terms of sensitivity and selectivity.
Article
Chemistry, Analytical
Ramon E. Cevallos-Cedeno, Guillermo Quinones-Reyes, Consuelo Agullo, Antonio Abad-Somovilla, Antonio Abad-Fuentes, Josep Mercader
Summary: Sensitive and selective immunological assays for anatoxin-a have been developed using homemade monoclonal antibodies. The assays have low detection limits, good recovery rates, and are applicable in different formats. A visual detection method with a relatively low limit of detection has also been optimized. The accuracy of the method has been validated by comparison with high-performance liquid chromatography-tandem mass spectroscopy data.
ANALYTICAL CHEMISTRY
(2022)
Article
Environmental Sciences
Daniel Lopez-Puertollano, Consuelo Agullo, Josep V. Mercader, Antonio Abad-Somovilla, Antonio Abad-Fuentes
Summary: This study successfully developed high-affinity monoclonal antibodies against dicamba and used immunoassay technology to develop a method for the rapid determination of dicamba residues in environmental water samples.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Chemistry, Analytical
Hadyn Duncan, Cristina Juan, Jordi Manes, Josep V. Mercader, Antonio Abad-Somovilla, Antonio Abad-Fuentes
Summary: A new method for the analysis of patulin in fruit products was developed in this study. The method is simple, sensitive, and does not require organic solvents. It has also been successfully applied under real conditions.
Article
Biochemical Research Methods
M. Munoz-Bartual, J. M. Herrero-Martinez, F. A. Esteve-Turrillas
Summary: A fast and simple method for synthesizing paper-immobilized molecularly imprinted polymers (MIPs) using a 405 nm laser pointer was proposed. The paper-MIP device allows the extraction and determination of methamphetamine in oral fluids and urine with high portability and minimal solvent consumption. The method provides a limit of detection of 14 μg L -1 and quantitative recoveries from 81 to 95%.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Evan Lesne, Miguel Munoz-Bartual, Francesc A. Esteve-Turrillas
Summary: A fast and simple procedure based on MEPS and LC-MS/MS was developed for quantifying 28 synthetic hallucinogens in oral fluids. The proposed method demonstrated high precision and quantitative recoveries, with limits of detection ranging from 0.09 to 1.22 µg/L. It is suitable for the determination of NBOMe derivatives and other synthetic hallucinogenic substances in oral fluid samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Correction
Food Science & Technology
Luis G. Addante-Moya, Antonio Abad-Somovilla, Antonio Abad-Fuentes, Consuelo Agullo, Josep V. Mercader
Article
Chemistry, Analytical
Simone Cavalera, Thea Serra, Antonio Abad-Fuentes, Josep V. Mercader, Antonio Abad-Somovilla, Fabio Di Nardo, Antonio D'Avolio, Amedeo De Nicolo, Valentina Testa, Matteo Chiarello, Claudio Baggiani, Laura Anfossi
Summary: Highly active antiretroviral therapy (HAART) can have toxic effects due to potent drugs like Tenofovir (TFV). Successful treatment relies on proper management of TFV, and therapeutic drug monitoring (TDM) of TFV concentrations in saliva can be a valuable tool for determining treatment compliance. We have developed a highly sensitive ELISA and LFIA for quantification and screening of TFV in saliva, respectively.
Article
Chemistry, Applied
M. Graca Dias, Elsa Vasco, Francisco Ravasco, Lufsa Oliveira
Summary: This study estimated the vitamin D intake of "adults" and "elderly" populations in Portugal using the TDS methodology. The results showed that the majority of people had inadequate vitamin D intake, well below the Dietary Reference Values.
Article
Chemistry, Applied
Yanan Wang, Jiachen Shi, Yong-Jiang Xu, Chin-Ping Tan, Yuanfa Liu
Summary: This study investigates the variations in lipid digestion profiles among individuals of different ages using in vitro digestion models. The findings suggest that adults have a more comprehensive lipid digestion compared to infants, and infants tend to release shorter chain length and more saturated free fatty acids during digestion. Additionally, the particle sizes in the stomach of the elderly were consistently larger. This study enhances our understanding of how lipids with different degrees of unsaturation undergo digestion in diverse age groups.
Article
Chemistry, Applied
Hyunjong Yu, Huisu Kim, Pahn-Shick Chang
Summary: Chitosan-pectin emulsion-filled hydrogel (EFH) was developed to enhance the bioaccessibility of lipophilic bioactive compounds through intestinal delivery. The EFH, prepared without crosslinking agents, demonstrated improved mechanical strength and compactness with higher pectin concentration. It retained the emulsion at pH 2.0 and released it at pH 7.4, resulting in enhanced release of free fatty acids and improved bioaccessibility of curcumin.
Article
Chemistry, Applied
Tongze Zhang, Siqi Hong, Jia-Rong Zhang, Pin-He Liu, Siyi Li, Zixian Wen, Jianwei Xiao, Guirong Zhang, Olivier Habimana, Nagendra P. Shah, Zhongquan Sui, Harold Corke
Summary: Lactic acid fermentation significantly affects the morphology and physicochemical properties of proso millet starch, including the formation of surface indentations and small pores, decrease in gelatinization temperatures, and changes in hardness and adhesiveness.
Article
Chemistry, Applied
Liqin Kong, Feng Hong, Peng Luan, Yiping Chen, Yaoze Feng, Ming Zhu
Summary: This study presents a novel impedance biosensor using composite nanomaterials and T1R1 as a signal probe, which can competitively and ultra-sensitively detect umami intensity. The biosensor exhibits exceptional analytical performance and is suitable for food flavor evaluation.
Article
Chemistry, Applied
Kunying Lv, Yixin Yang, Qilong Li, Ran Chen, Liang Deng, Yiwei Zhang, Ning Jiang
Summary: Horse's milk, with its high nutritional value and low allergenic proteins, could be a substitute for cow's milk in infant consumption. A proteomic method was used to identify and compare milk fat globule membrane (MFGM) and whey proteins from different horse breeds. The study found differences in protein composition and functionality, which could support the development of formulas more suitable for human infants.
Article
Chemistry, Applied
Enrique Jacobo Diaz-Montana, Helene Brignot, Ramon Aparicio-Ruiz, Thierry Thomas- Danguin, Maria Teresa Morales
Summary: Sensory perception of virgin olive oil is influenced by phenols and volatiles, which are affected by the composition of the oil and biological factors. This study investigated the effect of saliva and phenols on the release of volatiles, and found that the presence of phenols decreased the release of saturated volatiles.
Article
Chemistry, Applied
Wei Zhou, Rui Zhang, Zhen Cai, Fangfang Wu, Yong Hu, Chao Huang, Kun Hu, Yun Chen
Summary: Environmentally friendly and outstanding pH-responsive cationic starch nanoparticles (CSNP) were prepared from pH-sensitive starch. CSNP exhibited nanosize and regular sphere, highly free-flowing molecular chains, and demonstrated excellent pH responsiveness through multiple emulsion/demulsification transitions.
Article
Chemistry, Applied
Andrea Koo, Vinayak Ghate, Weibiao Zhou
Summary: This study suggests that direct seeding may negatively affect the nutritional quality of crops, causing a decrease in ascorbic acid, vitamin K, and total glucosinolate content.
Article
Chemistry, Applied
Tonghao Du, Yazhou Xu, Xiaoyan Xu, Shijin Xiong, Linli Zhang, Biao Dong, Jinqing Huang, Tao Huang, Muyan Xiao, Tao Xiong, Mingyong Xie
Summary: This study successfully improved the ACE inhibitory activity of black sesame seeds by fermenting them with Lactobacillus Plantarum NCU116 and hydrolyzing them using acid protease. The RF-PSO model was used to predict the ACE inhibitory activity during the hydrolysis process. Eight peptides with ACE inhibitory activity were identified from fermented black sesame seed hydrolysates after separation and screening.
Article
Chemistry, Applied
Yao Liu, Songyi Lin, Kexin Liu, Shan Wang, Qiaozhen Liu, Na Sun
Summary: This study analyzed the structural changes of shrimp proteins during digestion, predicted the immunodominant epitopes, and validated their allergenicity. The results showed that shrimp proteins were degraded into peptides during digestion, but still carried IgE epitopes that trigger allergic reactions.
Article
Chemistry, Applied
Tiantian Fu, Hongwei Cao, Yu Zhang, Xiao Guan
Summary: This study investigates the impact of milling on the active components in rice, with a focus on the stability and bioaccessibility of phenols, VB1, and alpha-GABA during cooking and digestion. The findings show that milling exacerbates the instability of gamma-GABA during cooking and VB1 during digestion, and it affects the bioaccessibility of these active compounds.
Article
Chemistry, Applied
Zhihao Yang, Yanru Hou, Min Zhang, Puxin Hou, Chang Liu, Lu Dou, Xiaoyu Chen, Lihua Zhao, Lin Su, Ye Jin
Summary: This study investigated the molecular mechanism of feeding regimes on lamb flavor by using TMT labeling combined with MS. The results showed that pasture-fed groups had higher levels of amino acids and volatile flavor substances compared to concentrate-fed groups. Additionally, several differentially abundant proteins associated with lamb flavor were identified.
Article
Chemistry, Applied
Zixuan Xie, De Zhang, Junyu Zhu, Qianqian Luo, Jun Liu, Jingtao Zhou, Xiaoyong Wang, Yuqiong Chen, Zhi Yu, Dejiang Ni
Summary: This study investigated the acidification of aroma-enhanced black tea during storage. Analysis of non-volatile substances and organic acids using UPLC-Q-TOF/MS and HPLC revealed a decrease in soluble sugars and amino acids, while an increase in organic acids such as oxalic acid, malic acid, and quinic acid. In vitro experiments further demonstrated that the acidification is a result of the decomposition of sugars and amino acids by heating, as well as the oxidation of aromatic aldehydes. Additionally, the study showed that the taste composition of tea infusion is altered, with reduced amino acids, catechins, soluble sugars, and flavonoids. This research provides a theoretical basis for improving the quality of black tea.
Article
Chemistry, Applied
Lin Wang, Falai Ma, Zihan Li, Yan Zhang
Summary: This study developed time-temperature integrators based on amyloglucosidase@Cu3(PO4)2 nanoflowers for monitoring the freshness of chilled pork. The results showed that the integrators were highly reliable and accurate in predicting the quality of chilled pork.
