Article
Reproductive Biology
Yijing He, Lei Peng, Jia Li, Qiao Li, Yajie Chu, Qixin Lin, Rong Rui, Shiqiang Ju
Summary: TPX2 plays an important role in meiotic spindle formation in porcine oocytes and interacts with microtubule-associated proteins AURKA and TACC3.
Article
Multidisciplinary Sciences
Jin-Gyeong Park, Hanul Jeon, Sangchul Shin, Chiman Song, Hyomin Lee, Nak-Kyoon Kim, Eunice EunKyeong Kim, Kwang Yeon Hwang, Bong-Jin Lee, In-Gyun Lee
Summary: This study identified a conserved region of CEP192 that interacts with AURKA and revealed the structural basis for CEP192-mediated regulation of AURKA at the centrosome. This regulation is distinct from TPX2-mediated regulation on the spindle microtubule.
Article
Biology
Richard Wang, Camilla Ascanelli, Ahmed Abdelbaki, Alex Fung, Tim Rasmusson, Iacovos Michaelides, Karen Roberts, Catherine Lindon
Summary: In this study, a PROTAC derivative of AURKA inhibitor MLN8237 was characterized for its efficient and specific destruction of AURKA. The subcellular localization and differential sensitivity of AURKA pools to PROTAC action were demonstrated, highlighting the potential of this new class of degrader compounds for targeting specific subpopulations within cells. The findings suggest that PROTACs can modulate AURKA functions in distinct cellular compartments, providing a novel approach for therapeutic intervention.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Aurelien Courtois, Shuhei Yoshida, Osamu Takenouchi, Kohei Asai, Tomoya S. Kitajima
Summary: This study demonstrates the importance of stable kinetochore-microtubule attachments in confining MTOC positions at spindle poles and limiting spindle elongation in acentriolar spindles. The gradual MTOC sorting process in the metaphase spindle is crucial for maintaining spindle integrity. Disruption of stable kinetochore-microtubule attachments leads to failure in MTOC confinement and spindle elongation termination.
Article
Cell Biology
Juan Ge, Na Zhang, Shoubin Tang, Feifei Hu, Xiaojing Hou, Hongzheng Sun, Longsen Han, Qiang Wang
Summary: Maternal diabetes impairs oocyte quality, but controlling the PDK1-mediated Ser232-PDHE1α phosphorylation pathway can partly protect oocytes from the effects of diabetes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Ellis L. Ryan, James Shelford, Teresa Massam-Wu, Richard Bayliss, Stephen J. Royle
Summary: The research identified TACC3 and clathrin as core members, and chTOG and GTSE1 as ancillary members of the complex. Additionally, PIK3C2A was shown not to be a part of the complex. Targeting the TACC3-clathrin interface or their microtubule-binding sites are the two most likely strategies to disrupt spindle stability mediated by this multiprotein complex.
JOURNAL OF CELL SCIENCE
(2021)
Article
Cell Biology
Hao Qin, Yi Qu, Yi-Feng Yuan, Yang-Yang Li, Jie Qiao
Summary: This study revealed that RBM14 is down-regulated in oocytes from old mice and shows overlapped localization patterns with alpha-tubulin in different stages of meiosis. Knockdown of RBM14 may result in symmetric division of oocytes, spindle defects, and chromosome abnormalities. Additionally, RBM14 interacts with endogenous alpha-tubulin and modulates oocyte meiotic maturation by affecting alpha-tubulin acetylation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Lin-Lin Hu, Meng-Hao Pan, Feng-Lian Yang, Zi-Ao Zong, Feng Tang, Zhen-Nan Pan, Xiang Lu, Yan-Ping Ren, Jun-Li Wang, Shao-Chen Sun
Summary: Research demonstrates that FASCIN is crucial for mouse oocyte maturation, affecting spindle migration and polar body extrusion. It may be regulated by PKC and plays a significant role in meiosis.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Si-Cheng Zhao, Feng-Xin Qiao, Ming-Xin Sun, Yue-Cen Liu, Hai-Long Wang, Zhi-Ran Xu, Yu Liu
Summary: This study found that cobalt chloride treatment affects ovary function, leading to decreased oocyte quality, impaired follicular growth, disrupted spindle assembly, and mitochondrial dysfunction.
Article
Biology
Marjan Abbasi, Alexander Julner, Yan Ting Lim, Tianyun Zhao, Radoslaw Mikolaj Sobota, Victoria Menendez-Benito
Summary: Phosphorylation of Spc110 at S11 and S36 is required for timely cell cycle progression in budding yeast. Non-phosphorylatable and double mutant Spc110 expression leads to abnormal spindle morphology and delayed cell cycle progression.
Article
Chemistry, Multidisciplinary
Kun-Huan Zhang, Yuan-Jing Zou, Meng-Meng Shan, Zhen-Nan Pan, Jia-Qian Ju, Jing-Cai Liu, Yi-Ming Ji, Shao-Chen Sun
Summary: This study reveals the noncanonical functions of ADP-ribosylation factor 1 (Arf1) in mouse oocyte meiosis, including its roles in G2/M transition and meiotic spindle organization.
Article
Reproductive Biology
Ying Xu, Chang -Long Xu, Zhong-Feng Xu, Xin-Jie Wang, Hui-Sheng Liang, Zhao-Cheng Zeng, Li-Xin Zeng, Kang-Na Wei, Shu-Zi Deng, Shu-Juan Xie, Jiang Jiang, Yu-Xin Liu, Yun-Kao Cao, Hai-Long Wang
Summary: Fbf1 plays a crucial role in spindle assembly and chromosome arrangement during mouse oocyte meiosis, affecting the progression of cell cycle.
Article
Cell Biology
Marilena Rizzo, Tom A. E. Stout, Santo Cristarella, Marco Quartuccio, Geert J. P. L. Kops, Marta De Ruijter-Villani
Summary: Aneuploidy originating during meiosis in oocytes is a major cause of reduced fertility, implantation failure, and miscarriage in women beyond their mid-thirties. The underlying molecular defects leading to error-prone chromosome segregation in older women's oocytes are not well understood, but loss of chromosome cohesion, defective microtubule dynamics, and spindle assembly are major contributors. In aged mares, gene expression for the kinases Mps1, Spc25, and AurkC is reduced, leading to instability in spindle (re)assembly and predisposing to chromosome segregation errors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biology
Italia Anna Asteriti, Federica Polverino, Venturina Stagni, Valentina Sterbini, Camilla Ascanelli, Francesco Davide Naso, Anna Mastrangelo, Alessandro Rosa, Alessandro Paiardini, Catherine Lindon, Giulia Guarguaglini
Summary: AurkA kinase is frequently overexpressed in tumors and its nuclear localization in interphase is correlated with its oncogenic potential. The mechanisms leading to AurkA nuclear accumulation are poorly explored, but it is influenced by the cell cycle phase and nuclear export, and not by its kinase activity. Co-overexpression of AURKA and TPX2 or impairment of proteasome activity can induce AurkA nuclear accumulation.
LIFE SCIENCE ALLIANCE
(2023)
Article
Medicine, Research & Experimental
Shuo Chao, Li -Jun Li, Jun Lu, Shu-Xian Zhao, Ming -Hui Zhao, Gui-An Huang, Shen Yin, Wei Shen, Qing-Yuan Sun, Yong Zhao, Zhao-Jia Ge, Lei Zhao
Summary: This study evaluated the effect of EGCG on the maturation of diabetic oocytes. The results showed that EGCG could reduce the frequency of abnormal spindle assembly and chromosome mismatches in diabetic oocytes. Additionally, EGCG alleviated abnormal mitochondrial membrane potential, regulated the expression of genes involved in mitochondrial fusion and fission, reduced reactive oxygen species levels, and decreased DNA damage in diabetic oocytes.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Cell Biology
Claudia Baumann, Xiaotian Wang, Luhan Yang, Maria M. Viveiros
JOURNAL OF CELL SCIENCE
(2017)
Article
Developmental Biology
Xiaotian Wang, Claudia Baumann, Rabindranath De La Fuente, Maria M. Viveiros
Article
Developmental Biology
Luhan Yang, Claudia Baumann, Rabindranth De La Fuente, Maria M. Viveiros
Article
Cell Biology
Claudia Baumann, Xiangyu Zhang, Rabindranath De La Fuente
JOURNAL OF CELL BIOLOGY
(2020)
Article
Multidisciplinary Sciences
Claudia Baumann, Wei Ma, Xiaotian Wang, Muthugapatti K. Kandasamy, Maria M. Viveiros, Rabindranath De La Fuente
NATURE COMMUNICATIONS
(2020)
Article
Genetics & Heredity
Claudia Baumann, Xiangyu Zhang, Ling Zhu, Yuhong Fan, Rabindranath De La Fuente
Summary: The study utilized CUT&RUN and ATAC-seq to investigate the changes in histone modifications and genome-wide chromatin accessibility after exposure to valproic acid (VPA), a known histone deacetylase inhibitor. The results showed that VPA treatment led to a significant increase in global histone acetylation, affecting nucleosome stability and influencing cellular morphogenesis. Additionally, VPA altered the levels of histone H1 subtypes and the histone H1/nucleosome ratio, suggesting initial differentiation events.
EPIGENETICS & CHROMATIN
(2021)
Article
Cell Biology
Luhan Yang, Claudia Baumann, Rabindranath De la Fuente, Maria M. Viveiros
Summary: The study demonstrates that both bisphenol A (BPA) and its substitute, bisphenol F (BPF), have detrimental effects on cell division and early embryo development. BPF was found to be more harmful to the early embryo than BPA, disrupting cytoskeletal integrity and remodeling crucial for embryonic development.
Article
Multidisciplinary Sciences
Nozomi Takahashi, Federica Franciosi, Enrico Maria Daldello, Xuan G. G. Luong, Peter Althoff, Xiaotian Wang, Marco Conti
Summary: In the absence of transcription, gene expression required for oocyte maturation is regulated by translation of preexisting mRNAs. A defective program of mRNA translation in oocyte is associated with maternal aging, leading to a decline in oocyte quality during aging. Dysfunction in the oocyte translation program is characterized by disrupted ribosome loading, altered translation of 3'-UTR-reporters, altered poly(A) length of mRNAs, decreased levels of Cpeb1 mRNA translation and protein, and de-repression of Ccnb1 translation in old oocytes.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Claudia Baumann, Xiangyu Zhang, Maria M. Viveiros, Rabindranath de la Fuente
Summary: This study reveals the important role of pericentric non-coding RNAs in regulating heterochromatin structure, chromosome segregation, and spindle organization during oocyte meiosis. Depletion of major satellite RNA leads to changes in pericentric heterochromatin structure, chromosome instability, and misalignment.