Article
Biochemistry & Molecular Biology
Roberto Rodriguez Madrera, Belen Suarez Valles
Summary: The article describes an analytical method for extraction and quantitative determination of specific compounds in plant material using high-power ultrasound extraction and HPLC-DAD quantification, showing good accuracy and precision. Attention should be paid to the sample/solvent ratio to avoid degradation of the compounds.
Article
Chemistry, Medicinal
Ryota Akatsuka, Michiho Ito
Summary: This study determined for the first time the content and distribution of cyanogenic glycosides in perilla, finding that prunasin was the most abundant cyanogenic glycoside. The content of prunasin in perilla leaves varied among strains regardless of leaf color or the main compound in the essential oils. Prunasin content was higher in fresh and young leaves compared to dried and mature leaves. The growth stage and season of perilla also influenced the prunasin content.
JOURNAL OF NATURAL MEDICINES
(2023)
Review
Agronomy
Insyirah-Hannah Ruhaizat-Ooi, Rabiatul-Adawiah Zainal-Abidin, Nur Syatila Ab Ghani, Nor Afiqah-Aleng, Hamidun Bunawan, Norfarhan Mohd-Assaad, Zeti-Azura Mohamed-Hussein, Sarahani Harun
Summary: This article discusses the complex interplay of two compounds (GSL and CG) co-occurring in papaya. It also discusses and describes the species-specific pathways in papaya and explores the transcription factors involved in regulating GSL and CG biosynthesis pathways.
Article
Multidisciplinary Sciences
Matthew S. Bramble, Neerja Vashist, Arthur Ko, Sambhawa Priya, Celeste Musasa, Alban Mathieu, D' Andre Spencer, Michel Lupamba Kasendue, Patrick Mamona Dilufwasayo, Kevin Karume, Joanna Nsibu, Hans Manya, Mary N. A. Uy, Brian Colwell, Michael Boivin, J. P. Banae Mayambu, Daniel Okitundu, Arnaud Droit, Dieudonne Mumba Ngoyi, Ran Blekhman, Desire Tshala-Katumbay, Eric Vilain
Summary: Konzo, a distinct upper motor neuron disease associated with cyanogenic diet and chronic malnutrition, predominantly affects children and women of childbearing age in sub-Saharan Africa. The gut microbiome structure is highly variable depending on region of sampling, and unique enrichments of bacterial species and functional pathways have been identified in prone regions of the Congo that potentially modulate the susceptibility of konzo. Using metagenomic profiling in 180 individuals from the Democratic Republic of the Congo, associations between the gut microbiome and konzo have been found.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Applied
Bao-Dong Cai, Jian-Yuan Wu, Ya-Li Bai, Yu-Qi Feng
Summary: A highly sensitive method for analysis of 4 cyanogenic glycosides (CNGs) in cold-pressed flaxseed oil was developed using cigarette filter fiber-based solid-phase extraction (SPE) and ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The method showed low detection limits and good recovery rates, and was successfully applied to the analysis of CNGs in nine different cold-pressed flaxseed oils.
Article
Chemistry, Analytical
Yun Ling, Yukun Ouyang, Ying Wang, Ziyi Zhou, Bingyu Wu, Zhangyi Shen
Summary: This study identified and characterized 32 compounds from the extract of Ilex asprella using HPLC-ESI-QTOF-MS/MS, including 4 phenolic glycosides and 28 triterpenoid glycosides. Among them, 10 compounds and 8 compounds are newly discovered in Ilex asprella and have not been reported previously in the literature, respectively. This research provides a basis for further studies on Ilex asprella.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Food Science & Technology
Lenka Ceslova, Katerina Pravcova, Miroslava Juricova, Jan Fischer
Summary: The study optimized rapid reversed-phase high performance liquid chromatography coupled to tandem mass spectrometry for the determination of phenolic compounds in mead. The most frequently occurred phenolic compounds in extracts included benzoic acid, caffeic acid, and salicylic acid. Some samples had low levels of phenolic compounds, with one sample showing a very high concentration of vanillin.
Article
Food Science & Technology
Imelda N. Monroy-Garcia, Irma Edith Carranza-Torres, Pilar Carranza-Rosales, Maria Oyon-Ardoiz, Ignacio Garcia-Estevez, Jesus Fernando Ayala-Zavala, Javier Moran-Martinez, Ezequiel Viveros-Valdez
Summary: This study found that wild fruits such as Ehretia tinifolia and Sideroxylon lanuginosum are rich in antioxidants and exhibit antioxidant, antiproliferative, and digestive enzymatic inhibition activities. The extracts from these fruits contain beneficial phytochemicals and have potential for developing functional foods.
Article
Food Science & Technology
Maria Eugenia Araujo Silva Oliveira, Luciana Ribeiro da Silva Lima, Millena Cristina Barros Santos, Nathalia Ferrari Fonseca de Sales, Renata Marenda Ferreira, Luiz Claudio Cameron, Jose Manoel Colombari Filho, Priscila Zaczuk Bassinello, Carlos Wanderlei Piler de Carvalho, Mariana Simoes Larraz Ferreira, Cristina Yoshie Takeiti
Summary: This study investigated the effects of short germination and milling on the physical properties, phenolic compounds, antioxidant activity, and amino acid composition of rice. The results showed that short germination and milling can improve the nutritional quality and antioxidant activity of rice. The husk + bran fraction had the highest level of total antioxidant activity, and the levels of glutamic acid and GABA were significantly increased. Short germination and milling can be an effective strategy to enhance the nutritional value of rice.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Food Science & Technology
Jose Thiago do Carmo Santos, Fabiane Cristina Petry, Eric de Castro Tobaruela, Adriana Zerlotti Mercadante, Maria Beatriz Abreu Gloria, Ana Maria Costa, Franco Maria Lajolo, Neuza Mariko Aymoto Hassimotto
Summary: Passiflora tenuifila, a Brazilian native passion fruit, is a dietary source of bioactive compounds with potential biological activity. The study found that the fruit is a good source of dietary fiber and maintains its bioactive compound contents and nutritional value at both mature-green and ripe stages. Carotenoids were responsible for the separation of the two ripening stages.
FOOD RESEARCH INTERNATIONAL
(2021)
Review
Biochemistry & Molecular Biology
Ewa Jaszczak-Wilke, Zaneta Polkowska, Marek Koprowski, Krzysztof Owsianik, Alyson E. Mitchell, Piotr Balczewski
Summary: Amygdalin is a natural cyanogenic glycoside found in the seeds of some edible plants, with both anticancer activity and toxicity due to hydrogen cyanide production. Despite studies on cancer cell lines, clinical evidence for its anticancer properties is not fully confirmed. High dose exposures to amygdalin can lead to cyanide toxicity.
Article
Food Science & Technology
Marianna Giancaterino, Thomas Fauster, Anna Krottenthaler, Henry Jaeger
Summary: This study investigates the use of pulsed electric fields (PEF) as a pre-treatment method to improve the debittering process and facilitate the degradation of cyanide precursors in cherry kernels. Different PEF treatments were performed at a constant electric field strength of 2.2 kV/cm, with varying energy inputs. The results show that PEF-treated samples have higher and faster detoxification efficiency, as well as minimizing sugar loss during the debittering process.
INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES
(2023)
Article
Plant Sciences
Mariana Cecilia Grohar, Aljaz Medic, Tea Ivancic, Robert Veberic, Jernej Jogan
Summary: Taxonomic delimitation of certain species in the genus Phyteuma can be challenging due to highly variable morphological traits and hybridization. This study presents a new approach using detailed analysis of flower color variability and chemical fingerprinting to clarify taxonomic statuses. The most comprehensive metabolic research on the genus Phyteuma was conducted, identifying and quantifying 44 phenolic compounds and revealing their potential role in flower color variability.
Article
Green & Sustainable Science & Technology
Clara Miguez, Angeles Cancela, Xana Alvarez, Angel Sanchez
Summary: Phenolic compounds extracted from Tradescantia fluminensis have potential applications in pharmaceutical and food preservation due to their high antioxidant potential. However, the current management of bio-waste resulting from invasive species control actions is not fully effective.
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Biochemistry & Molecular Biology
Zening Wang, Colin J. Barrow, Frank R. Dunshea, Hafiz A. R. Suleria
Summary: Five Australian grown pear varieties were selected for this study, with Beurre Bosc and Josephine de Malines exhibiting high levels of phenolic compounds, suggesting potential health benefits.
Article
Food Science & Technology
Aljaz Medic, Tilen Zamljen, Ana Slatnar, Metka Hudina, Mariana Cecilia Grohar, Robert Veberic
Summary: This study aimed to investigate the yield, quality, metabolic responses, and potential toxicity of Cucumis sativus grown in juglone-containing soils. It was found that a concentration of 100 mu M of juglone or the same concentration of walnut leaf extract does not affect the yield of C. sativus, while a concentration of 1 mM of juglone strongly affects it. Juglone itself was found only in the roots of C. sativus, but not in the leaves or fruits, making C. sativus fruits considered safe for cultivation in juglone-containing soils.
Article
Plant Sciences
Nina Kunc, Metka Hudina, Joze Bavcon, Branko Vres, Zlata Luthar, Kristina Gostincar, Maja Mikulic-Petkovsek, Gregor Osterc, Blanka Ravnjak
Summary: The study investigated the petals of R. pendulina, R. spinosissima, and their hybrid Rosa pendulina x spinosissima collected in western Slovenia. Morphometric analysis and genetic analysis were used to identify the roses and determine genome size. Liquid chromatography and mass spectrometry were used to measure the phenolic compound content. Flow cytometry confirmed native hybridization. The hybrid's genome size was similar to the parent plants, but the parent plants had significantly different genome sizes. Phenolic compound content decreased after crossing, with the highest content in R. spinossisima, lowest in the hybrid, and intermediate in R. pendulina. Flavanol and flavonol content was lowest in the hybrid petals, while anthocyanin content was highest.
Article
Plant Sciences
Djordje Boskov, Dragan Milatovic, Vera Rakonjac, Gordan Zec, Metka Hudina, Robert Veberic, Maja Mikulic-Petkovsek
Summary: This study investigated the influence of three sweet cherry cultivars grafted on six rootstocks on the phenolic profile of the fruits. A total of 54 phenolic compounds were identified, with anthocyanins and hydroxycinnamic acids being the most abundant. 'Kordia' had the highest phenolic content among the cultivars. The choice of rootstock also had a significant impact on the phenolic content, with 'Colt' and 'Gisela 5' promoting the highest values in 'Kordia' and 'Carmen', and 'Oblacinska' and 'M x M 14' inducing the highest values in 'Regina'. The study highlights the importance of cultivar and rootstock selection for improving the phenolic content and nutritional value of sweet cherry fruits.
Article
Plant Sciences
Maja Mikulic-Petkovsek, Anton Ivancic, Sasa Gacnik, Robert Veberic, Metka Hudina, Silvija Marinovic, Christian Molitor, Heidi Halbwirth
Summary: This study analyzed the changes in sugar content, organic acids, phenolic compounds, and selected enzyme activities in the anthocyanin pathway in NIGRA (Sambucus nigra var. nigra-black fruits) and VIRIDIS (S. nigra var. viridis-green fruits) fruits at four stages of ripening. The results showed that glucose and fructose content in green fruits were higher than in colored fruits, and sugar content increased significantly until the third developmental stage. NIGRA berries had a high content of anthocyanins and flavonols, while flavonols were the major phenolic group in VIRIDIS fruits. NIGRA fruits showed strong activation of the late anthocyanin pathway, while VIRIDIS fruits lacked anthocyanins and only had one flavanol.
Article
Biochemistry & Molecular Biology
Marjeta Mencin, Nika Markanovic, Maja Mikulic Petkovsek, Robert Veberic, Petra Terpinc
Summary: The study investigated the use of bioprocessed wholegrain spelt flour as a partial substitute for white wheat flour in bread recipes. The addition of 1% and pasteurised 5% germinated + enzymatic treated spelt flour significantly improved the bread's specific volume, but not its texture and sensory evaluation. Increasing the percentage of bioprocessed spelt flour led to a darker bread color and decreased quality. Breads with 2.5% and 5% germinated + fermented spelt flour had the most acceptable characteristics and improved antioxidant content.
Article
Biochemistry & Molecular Biology
Marjeta Mencin, Nika Markanovic, Maja Mikulic-Petkovsek, Robert Veberic, Petra Terpinc
Summary: The study aimed to investigate whether replacing wheat flour with bioprocessed spelt flour enhances the bioaccessibility of antioxidants in bread. The results demonstrated that the type and amount of bioprocessed spelt flour significantly influenced the extractable and bound total phenolic content, individual phenolics, antioxidant activity, and bioaccessibility after in vitro digestion. While certain phenolic acids decreased after digestion, others increased. Notably, the digested bread made with 5% germinated + fermented spelt flour contained significantly higher levels of trans-ferulic acid compared to the control bread. Overall, incorporating germinated and fermented or enzymatically treated spelt flour in white bread recipes could be a promising way to offer nutritionally valuable foods to consumers.
Article
Agronomy
Urszula Barbara Baluszynska, Maria Licznar-Malanczuk, Aljaz Medic, Robert Veberic, Mariana Cecilia Grohar
Summary: This study aimed to assess the impact of two agrotechnical factors (floor management and nitrogen dose) on the yield and primary and secondary metabolites in 'Sampion' apple fruit. Living mulch did not decrease yield compared to herbicide fallow. Apples were found to contain a rich composition of phenolic and volatile organic compounds, which varied with the evaluated factors and weather conditions. Precipitation deficit and high summer temperatures hindered fruit growth and development, resulting in a higher content of phenolic compounds in the fruit flesh from trees in herbicide fallow. Living mulch, acting as a regulator of nitrogen availability to trees, stimulated anthocyanin synthesis, which was enhanced by low average temperatures at harvest time, leading to extensive fruit skin red blush.
Article
Horticulture
Kristyna Simkova, Robert Veberic, Metka Hudina, Nika Cvelbar Weber, Tina Smrke, Mariana Cecilia Grohar, Tea Ivancic, Massimiliano Pelacci, Aljaz Medic, Jerneja Jakopic
Summary: This study investigated the differences in the distribution of primary and secondary metabolites in four strawberry cultivars. Results showed that the outer part of the fruit had lower pH, higher organic acid content, and lower glucose and fructose content. Differences were also observed in the total phenolic and anthocyanin contents, which were always higher in the outer part. The study emphasizes the need for proper mixing of samples before extractions to obtain representative results.
Article
Horticulture
Tina Smrke, Natasa Stajner, Tjasa Cesar, Robert Veberic, Metka Hudina, Jerneja Jakopic
Summary: A non-destructive method using a DA-meter to measure chlorophyll absorbance (I-AD) has been used to assess blueberry fruit maturity directly on the plant. This study examined fruit quality parameters, chemical composition, and gene expression at different maturity stages, and found significant changes in all observed parameters during ripening, with high correlation to I-AD values. Peel lightness, chroma, fruit firmness, and organic acids decreased, while sugars, sugar/organic acid ratio, total phenolics, and relative expression of ANS increased during ripening.
Article
Food Science & Technology
Aljaz Medic, Anita Solar, Metka Hudina, Robert Veberic, Tilen Zamljen
Summary: This study aimed to investigate the effects of compost made from walnut and hazelnut leaves on plant growth. The results showed that compost containing walnut and hazelnut leaves inhibited lettuce yield, while compost without these leaves promoted lettuce yield. Therefore, we recommend using dry walnut leaves and cut grass for composting, and not using hazelnut leaves.
Article
Food Science & Technology
Jan Juhart, Aljaz Medic, Jerneja Jakopic, Robert Veberic, Metka Hudina, Franci Stampar
Summary: The aim of this experiment was to compare the color and metabolic profile of apple juice from two different cultivars: the red-fleshed 'Baya Marisa' and the white-fleshed 'Golden Delicious'. The changes in phenolic compounds, organic acids, and sugar content were analyzed after pasteurization and one year of storage under different conditions. A total of 26 individual phenolic compounds were identified and quantified. The total analyzed phenolics content decreased after pasteurization for both cultivars. The sucrose content remained unchanged after pasteurization but significantly decreased after one year of storage, while the fructose and glucose content remained the same after pasteurization and increased significantly after one year of storage for both cultivars.
Article
Food Science & Technology
Maja Mikulic-Petkovsek, Katja Jakljevic, Robert Veberic, Metka Hudina, Denis Rusjan
Summary: The present study compares traditional fruit processing methods on medlar fruits and their effects on sugar content, organic acids, and phenolic composition. It aims to determine suitable processing methods for technologically mature yet inedible medlar fruits while maintaining their good chemical quality. Analysis shows that fructose and glucose are the major sugars in medlars, while malic acid and quinic acid are the most abundant organic acids. A total of 38 phenolic compounds were identified, with phenolic acids and flavanols predominating. Different fruit treatments resulted in variations in metabolite content, with heated and edible fruits showing lower levels of fructose and sorbitol. Edible fruits had lower content of organic acids, flavanols, and phenolic acids compared to technologically ripe fruits. Heating the fruits increased total phenolic compounds. Exposure to hoarfrost minimally affected the chemical quality of medlar fruits. Processing medlar fruit with hoarfrost remains the most suitable method of fruit bletting.
Article
Horticulture
Valentina Schmitzer, Franci Stampar, Anze Turk, Jerneja Jakopic, Metka Hudina, Robert Veberic, Tina Smrke
Summary: The aim of this research was to evaluate the effect of a combination of mycorrhizal and bacterial biostimulants on the performance of 'Clery' strawberries. The results showed that the application of these products significantly improved the vegetative growth and fruit characteristics, leading to a more than 30% increase in yield. Although there was a minor decrease in certain nutritional components, the color characteristics and essential compounds of the fruit were not affected.
Article
Chemistry, Applied
M. Graca Dias, Elsa Vasco, Francisco Ravasco, Lufsa Oliveira
Summary: This study estimated the vitamin D intake of "adults" and "elderly" populations in Portugal using the TDS methodology. The results showed that the majority of people had inadequate vitamin D intake, well below the Dietary Reference Values.
Article
Chemistry, Applied
Yanan Wang, Jiachen Shi, Yong-Jiang Xu, Chin-Ping Tan, Yuanfa Liu
Summary: This study investigates the variations in lipid digestion profiles among individuals of different ages using in vitro digestion models. The findings suggest that adults have a more comprehensive lipid digestion compared to infants, and infants tend to release shorter chain length and more saturated free fatty acids during digestion. Additionally, the particle sizes in the stomach of the elderly were consistently larger. This study enhances our understanding of how lipids with different degrees of unsaturation undergo digestion in diverse age groups.
Article
Chemistry, Applied
Hyunjong Yu, Huisu Kim, Pahn-Shick Chang
Summary: Chitosan-pectin emulsion-filled hydrogel (EFH) was developed to enhance the bioaccessibility of lipophilic bioactive compounds through intestinal delivery. The EFH, prepared without crosslinking agents, demonstrated improved mechanical strength and compactness with higher pectin concentration. It retained the emulsion at pH 2.0 and released it at pH 7.4, resulting in enhanced release of free fatty acids and improved bioaccessibility of curcumin.
Article
Chemistry, Applied
Tongze Zhang, Siqi Hong, Jia-Rong Zhang, Pin-He Liu, Siyi Li, Zixian Wen, Jianwei Xiao, Guirong Zhang, Olivier Habimana, Nagendra P. Shah, Zhongquan Sui, Harold Corke
Summary: Lactic acid fermentation significantly affects the morphology and physicochemical properties of proso millet starch, including the formation of surface indentations and small pores, decrease in gelatinization temperatures, and changes in hardness and adhesiveness.
Article
Chemistry, Applied
Liqin Kong, Feng Hong, Peng Luan, Yiping Chen, Yaoze Feng, Ming Zhu
Summary: This study presents a novel impedance biosensor using composite nanomaterials and T1R1 as a signal probe, which can competitively and ultra-sensitively detect umami intensity. The biosensor exhibits exceptional analytical performance and is suitable for food flavor evaluation.
Article
Chemistry, Applied
Kunying Lv, Yixin Yang, Qilong Li, Ran Chen, Liang Deng, Yiwei Zhang, Ning Jiang
Summary: Horse's milk, with its high nutritional value and low allergenic proteins, could be a substitute for cow's milk in infant consumption. A proteomic method was used to identify and compare milk fat globule membrane (MFGM) and whey proteins from different horse breeds. The study found differences in protein composition and functionality, which could support the development of formulas more suitable for human infants.
Article
Chemistry, Applied
Enrique Jacobo Diaz-Montana, Helene Brignot, Ramon Aparicio-Ruiz, Thierry Thomas- Danguin, Maria Teresa Morales
Summary: Sensory perception of virgin olive oil is influenced by phenols and volatiles, which are affected by the composition of the oil and biological factors. This study investigated the effect of saliva and phenols on the release of volatiles, and found that the presence of phenols decreased the release of saturated volatiles.
Article
Chemistry, Applied
Wei Zhou, Rui Zhang, Zhen Cai, Fangfang Wu, Yong Hu, Chao Huang, Kun Hu, Yun Chen
Summary: Environmentally friendly and outstanding pH-responsive cationic starch nanoparticles (CSNP) were prepared from pH-sensitive starch. CSNP exhibited nanosize and regular sphere, highly free-flowing molecular chains, and demonstrated excellent pH responsiveness through multiple emulsion/demulsification transitions.
Article
Chemistry, Applied
Andrea Koo, Vinayak Ghate, Weibiao Zhou
Summary: This study suggests that direct seeding may negatively affect the nutritional quality of crops, causing a decrease in ascorbic acid, vitamin K, and total glucosinolate content.
Article
Chemistry, Applied
Tonghao Du, Yazhou Xu, Xiaoyan Xu, Shijin Xiong, Linli Zhang, Biao Dong, Jinqing Huang, Tao Huang, Muyan Xiao, Tao Xiong, Mingyong Xie
Summary: This study successfully improved the ACE inhibitory activity of black sesame seeds by fermenting them with Lactobacillus Plantarum NCU116 and hydrolyzing them using acid protease. The RF-PSO model was used to predict the ACE inhibitory activity during the hydrolysis process. Eight peptides with ACE inhibitory activity were identified from fermented black sesame seed hydrolysates after separation and screening.
Article
Chemistry, Applied
Yao Liu, Songyi Lin, Kexin Liu, Shan Wang, Qiaozhen Liu, Na Sun
Summary: This study analyzed the structural changes of shrimp proteins during digestion, predicted the immunodominant epitopes, and validated their allergenicity. The results showed that shrimp proteins were degraded into peptides during digestion, but still carried IgE epitopes that trigger allergic reactions.
Article
Chemistry, Applied
Tiantian Fu, Hongwei Cao, Yu Zhang, Xiao Guan
Summary: This study investigates the impact of milling on the active components in rice, with a focus on the stability and bioaccessibility of phenols, VB1, and alpha-GABA during cooking and digestion. The findings show that milling exacerbates the instability of gamma-GABA during cooking and VB1 during digestion, and it affects the bioaccessibility of these active compounds.
Article
Chemistry, Applied
Zhihao Yang, Yanru Hou, Min Zhang, Puxin Hou, Chang Liu, Lu Dou, Xiaoyu Chen, Lihua Zhao, Lin Su, Ye Jin
Summary: This study investigated the molecular mechanism of feeding regimes on lamb flavor by using TMT labeling combined with MS. The results showed that pasture-fed groups had higher levels of amino acids and volatile flavor substances compared to concentrate-fed groups. Additionally, several differentially abundant proteins associated with lamb flavor were identified.
Article
Chemistry, Applied
Zixuan Xie, De Zhang, Junyu Zhu, Qianqian Luo, Jun Liu, Jingtao Zhou, Xiaoyong Wang, Yuqiong Chen, Zhi Yu, Dejiang Ni
Summary: This study investigated the acidification of aroma-enhanced black tea during storage. Analysis of non-volatile substances and organic acids using UPLC-Q-TOF/MS and HPLC revealed a decrease in soluble sugars and amino acids, while an increase in organic acids such as oxalic acid, malic acid, and quinic acid. In vitro experiments further demonstrated that the acidification is a result of the decomposition of sugars and amino acids by heating, as well as the oxidation of aromatic aldehydes. Additionally, the study showed that the taste composition of tea infusion is altered, with reduced amino acids, catechins, soluble sugars, and flavonoids. This research provides a theoretical basis for improving the quality of black tea.
Article
Chemistry, Applied
Lin Wang, Falai Ma, Zihan Li, Yan Zhang
Summary: This study developed time-temperature integrators based on amyloglucosidase@Cu3(PO4)2 nanoflowers for monitoring the freshness of chilled pork. The results showed that the integrators were highly reliable and accurate in predicting the quality of chilled pork.