The Corynebacterium diphtheriae HbpA Hemoglobin-Binding Protein Contains a Domain That Is Critical for Hemoprotein Binding, Cellular Localization, and Function

The acquisition of hemin iron from hemoglobin-haptoglobin (Hb-Hp) by Corynebacterium diphtheriae requires the iron-regulated surface proteins HtaA, ChtA, and ChtC and the recently identified Hb-Hp-binding protein, HbpA. We previously showed that a purified form of HbpA (HbpA-S), lacking the C-terminal region, was able to bind Hb-Hp. In this study, we show that the C-terminal region of HbpA significantly enhances binding to Hb-Hp. A purified form of HbpA that includes the Cterminal domain (HbpA-FL) exhibits much stronger binding to Hb-Hp than HbpA-S. Size exclusion chromatography (SEC) showed that HbpA-FL as well as HtaA-FL, ChtAFL, and ChtC-FL exist as high-molecular-weight complexes, while HbpA-S is present as a monomer, indicating that the C-terminal region is required for formation of large aggregates. Growth studies showed that expression of HbpA-FL in the DhbpA mutant restored wild-type levels of growth in low-iron medium that contained HbHp as the sole iron source, while HbpA-S failed to complement the Delta hbpA mutant. Protein localization studies in C. diphtheriae showed that HbpA-FL is present in both the supernatant and membrane fractions and that the C-terminal region is required for membrane anchoring. Purified HbpA-FL was able to enhance growth of the DhbpA mutant when added to culture medium that contained Hb-Hp as a sole iron source, suggesting that secreted HbpA is involved in the use of hemin iron from HbHp. These studies extend our understanding of this novel Hb-Hp binding protein in this important human pathogen. IMPORTANCE Hemoproteins, such as Hb, are an abundant source of iron in humans and are proposed to be required by numerous pathogens to cause disease. In this report, we expand on our previous studies in further defining the role of HbpA in hemin iron acquisition in C. diphtheriae. HbpA is unique to C. diphtheriae and appears to function unlike any previously described bacterial iron-regulated Hb- or Hb-Hp-binding protein. HbpA is both secreted and present in the membrane and exists as a large aggregate that enhances its ability to bind Hb-Hp and promote hemin iron uptake. Current studies with HbpA will increase our understanding of iron transport systems in C. diphtheriae.

Automated summary

This study demonstrates that the C-terminal region of HbpA significantly enhances the binding to Hb-Hp, with the purified form of HbpA-FL showing much stronger binding compared to HbpA-S. HbpA-FL is able to restore wild-type growth levels in low-iron medium for the DhbpA mutant, while HbpA-S fails to complement the Delta hbpA mutant. The localization of HbpA-FL in both the supernatant and membrane fractions of C. diphtheriae indicates the requirement of the C-terminal region for membrane anchoring.