期刊
FOOD AND CHEMICAL TOXICOLOGY
卷 88, 期 -, 页码 32-39出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2015.11.022
关键词
Alternariol; Cell cycle; Membrane potential; Necrosis-apoptosis; Degradation products; LC-MS/MS; Caco-2 cells
资金
- Economy and Competitiveness Spanish Ministry [AGL2013-43194-P]
Alternariol (AOH), a mycotoxin produced by Alternaria sp, appears as food contaminant in fruit, vegetables and cereal products. Its toxicity has been demonstrated, but the mechanisms involved have not been elucidated yet. In this study, the pathways triggered by AOH and degradation products generated on Caco-2 cells were evaluated. Cells were exposed to AOH sub-cytotoxic concentrations of 15, 30 and 60 mu M. Cell cycle disruption, the induction of apoptosis/necrosis and changes in mitochondrial membrane potential (Delta psi m) after 24 and 48 h was asses by flow cytometry. Also, AOH and its degradation products were evaluated after 24 and 48 h by high-performance liquid chromatography with tandem mass spectrometric (LC-MS/MS) to detect and quantify its levels. Cell cycle was significantly decreased at G1 phase and increased at S and G2/M phase at the time of exposure. AOH induced necrosis, apoptosis/necrosis and loss of Delta psi m in a dose and time-dependent manner. The concentrations of AOH quantified in the culture media exposed to AOH decreased as the exposure time was increased. In conclusion, AOH caused cytotoxic effects supported by blocking cell cycle, decreasing cell proliferation and increasing apoptosis/necrosis cells. (C) 2015 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据