期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 69, 期 35, 页码 10371-10378出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.1c04030
关键词
antibiotic detection; biosensors; aptamers; DNAzyme; food analysis
资金
- National Natural Science Foundation of China [22076043]
- Science and Technology Foundation for Excellent Creative Research Group of Hubei Provincial Department of Education [T201810]
The proposed method combines Exo III-catalyzed release of a Zn2+-dependent ligation DNAzyme with DNAzyme-driven strand displacement reaction for kanamycin detection. This method offers wide linear range, low detection limit, and excellent selectivity, repeatability, reliability, and convenience, making it promising for food quality monitoring applications.
Herein, we combine the exonuclease III (Exo III)-catalyzed release of a Zn2+-dependent ligation DNAzyme with the DNAzyme-driven strand displacement reaction (SDR) to develop a novel homogeneous colorimetric bioassay method for kanamycin (Kana) antibiotic detection. Upon the biorecognition reaction between Kana and a designed hairpin DNA, the DNAzyme-containing strand can be catalytically released by Exo III. Then, this DNAzyme will catalyze the ligation of two oligonucleotides to cause a SDR and the aggregation of gold nanoparticles (Au NPs) labeled by two linker DNA strands. Due to the aggregation of Au NPs for colorimetric signal transduction and the Exo III and SDR-assisted dual signal amplification, this method shows a wide linear range of 5 orders of magnitude and a very low detection limit down to 8.1 fg mL(-1). Together with its excellent selectivity, repeatability, reliability, and convenient manipulation, the proposed method shows a great potential for the food quality monitoring application.
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