Article
Biochemistry & Molecular Biology
Xiaochun Zeng, Yiou Pan, Fayi Tian, Jianyi Li, Hongfei Xu, Xuemei Liu, Xuewei Chen, Xiwu Gao, Tianfei Peng, Rui Bi, Qingli Shang
Summary: The study identified synergists that enhance the toxicity of certain insecticides, observed upregulation of UGT genes in resistant strains, and demonstrated the role of specific P450 and UGT genes in conferring resistance to certain insecticides. Transgenic expression in Drosophila melanogaster confirmed the involvement of certain genes in mediating insecticide resistance.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Xueke Gao, Xiangzhen Zhu, Chuanpeng Wang, Li Wang, Kaixin Zhang, Dongyang Li, Jichao Ji, Lin Niu, Junyu Luo, Jinjie Cui
Summary: This study characterized AgoCYP6CY19, a CYP gene in Aphis gossypii, and found that its expression is related to developmental stages and host specialization. Silencing AgoCYP6CY19 increased mortality and reduced fecundity in aphids. Additionally, AgoCYP6CY19 was induced by certain plant substances. These findings highlight the importance of AgoCYP6CY19 in detoxification and metabolism processes.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Entomology
Li Wang, Li Cui, Qinqin Wang, Yanpeng Chang, Weiling Huang, Changhui Rui
Summary: The sulfoxaflor-resistant A. gossypii strain developed cross-resistance to neonicotinoid, pyrethroid, and carbamate insecticides. The resistance was associated with increased levels of cytochrome P450 monooxygenase, GST, and carboxylesterase activities, as well as overexpression of P450 genes, especially CYP6CY13-2. Additionally, the resistant strain showed enhanced probing behavior and higher fecundity compared to the susceptible aphids.
JOURNAL OF PEST SCIENCE
(2022)
Article
Agriculture, Multidisciplinary
Yuntong Lv, Shuyun Wen, Yaping Ding, Xiwu Gao, Xuewei Chen, Kunpeng Yan, Fengting Yang, Yiou Pan, Qingli Shang
Summary: Field populations of Aphis gossypii have developed high resistance to neonicotinoids and the involvement of multiple cytochrome P450 genes in this resistance has been identified.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Entomology
Farman Ullah, Hina Gul, Kaleem Tariq, Muhammad Hafeez, Nicolas Desneux, Dunlun Song
Summary: This study uncovered the role of the CYP6CY14 and CYP6DC1 genes in the development of clothianidin resistance in the cotton-melon aphid. Silencing these genes increased the sensitivity of the aphids to clothianidin, indicating their involvement in detoxification mechanisms. These findings emphasize the importance of these genes in insecticide resistance development.
ENTOMOLOGIA GENERALIS
(2023)
Article
Biochemistry & Molecular Biology
Hongfei Xu, Yiou Pan, Jianyi Li, Fengting Yang, Xuewei Chen, Xiwu Gao, Shuyuan Wen, Qingli Shang
Summary: In this study, we found that AgoCSP1, AgoCSP4, and AgoCSP5 were overexpressed in an insecticide-resistant strain of Aphis gossypii and showed higher expression in broad body tissue than in the midgut. Suppression of these AgoCSPs increased the sensitivity of resistant aphids to cyantraniliprole. Transgenic Drosophila melanogaster expressing these AgoCSPs showed higher tolerance to cyantraniliprole.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Qiuling Tang, Xuchao Li, Yanping He, Kangsheng Ma
Summary: The study reveals the significant role of the CPR gene in insecticide resistance in Aphis gossypii. The expression of AgCPR is influenced by the developmental stage and concentration of insecticide, and silencing of AgCPR increases susceptibility to the insecticide, indicating its functional importance.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Huihui Zhang, Hengli Yang, Wenyang Dong, Zixuan Gu, Cuicui Wang, Anqi Chen, Xueyan Shi, Xiwu Gao
Summary: The highly resistant strain TMXR of melon aphids showed mutations in nAChR beta 1 subunit, decreased expression levels of the gene, and higher expression levels of P450s genes compared to susceptible strains. The involvement of nAChRs and CYP450s in the resistance mechanisms were highlighted, suggesting potential targets for overcoming insecticide resistance in Aphis gossypii.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Jianyi Li, Long Jin, Yuntong Lv, Yaping Ding, Kunpeng Yan, Hang Zhang, Yiou Pan, Qingli Shang
Summary: This study identifies the important roles of cytochrome P450 genes in detoxification of xenobiotics, particularly cyantraniliprole and gossypol, in Spodoptera litura. Several candidate genes were found to confer tolerance to these substances, while knockdown of certain genes increased mortality. Homology modeling and molecular docking analysis indicate the potential of these genes to bind with cyantraniliprole. Overall, this research provides important insights into the detoxification mechanisms and tolerance of S. litura.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Yuntong Lv, Yiou Pan, Jianyi Li, Yaping Ding, Zihan Yu, Kunpeng Yan, Qingli Shang
Summary: Clarifying the molecular mechanisms of cotton aphid resistance to various insecticides is crucial for safe insecticide application. This study reveals that ABCB5, ABCF2, and MRP12 are negatively regulated by the transcription factor CF2-II, leading to increased resistance to certain insecticides. These findings contribute to a better understanding of the adaptation mechanism of ABC transporters in the context of multi-insecticide resistance.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Entomology
Ren Li, Shenhang Cheng, Zhibin Chen, Tianfeng Guo, Pingzhuo Liang, Congai Zhen, Jinghui Wang, Lei Zhang, Pei Liang, Xiwu Gao
Summary: This study found that broflanilide has high insecticidal activity against Aphis gossypii and established a susceptible baseline for future resistance monitoring. These results are significantly useful for the rational chemical control of cotton aphids.
Article
Biochemistry & Molecular Biology
Zanrong Wen, Kaixin Li, Wei Xu, Zhuang Zhang, Ni Liang, Moxian Chen, Lei Guo
Summary: The sweet potato whitefly is a significant insect pest that transmits numerous plant viruses and causes damage to cotton and Solanaceae vegetables. This study found that miRNA miR-276-3p can regulate the resistance of B. tabaci to the insecticide cyantraniliprole by targeting the gene CYP6CX3. Additionally, exposure to cyantraniliprole led to the downregulation of genes associated with miRNA biogenesis. These findings enhance our understanding of the role of miRNAs in P450 regulation and cyantraniliprole resistance in B. tabaci.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Hongfei Xu, Kunpeng Yan, Yaping Ding, Yuntong Lv, Jianyi Li, Fengting Yang, Xuewei Chen, Xiwu Gao, Yiou Pan, Qingli Shang
Summary: In-depth research on chemosensory proteins (CSPs) revealed that CSPs play a crucial role in cotton aphids' resistance to pesticides. Functional verification of CSP1 and CSP4 in vivo and in vitro demonstrated that silencing CSPs through RNA interference significantly increased the sensitivity of resistant strains to corresponding pesticides. Transgenic Drosophila expressing CSPs exhibited varying tolerance mechanisms for different pesticides, indicating a potential role of overexpressed CSPs beyond pesticide resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agronomy
John W. Mahas, Todd D. Steury, Anders S. Huseth, Alana L. Jacobson
Summary: An investigation was conducted to characterize the susceptibility of 43 cotton aphid populations to imidacloprid over a 2-year period. The study found variable levels of resistance in the populations, with higher resistance associated with greater cotton production intensity. Rotating modes of action and limiting insecticide use are recommended to delay the evolution of insecticide resistance in cotton aphid populations.
PEST MANAGEMENT SCIENCE
(2023)
Article
Entomology
Rafaela Morando, Ivana Fernandes da Silva, Alisson da Silva Santana, Guilherme Sicca Lopes Sampaio, Andre Luiz Lourencao, Edson Luiz Lopes Baldin
Summary: The study identified cotton genotypes with varying degrees of resistance against A. gossypii aphids, with some genotypes showing strong abilities to inhibit aphid colonization and growth. These findings provide important insights for the development of insect-resistant cotton cultivars.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2021)