期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 22, 期 16, 页码 -出版社
MDPI
DOI: 10.3390/ijms22168606
关键词
spinal cord injury; rat; transcriptomic; bioinformatic analysis
资金
- POR-FESR 2019-21, project Mat2Rep, Emilia Romagna Region
- Cluster Tecnologici Nazionali, project IRMI, MIUR
- program Alte Competenze by Emilia Romagna Region
- Fondazione Montecatone, Imola (Italy)
In this study, the modification of 80 genes involved in synaptic plasticity after traumatic spinal cord injury (SCI) was investigated at different times using bioinformatic analysis. The results showed different gene expression regulation in spinal cord segments rostral and caudal to the lesion, as well as long-lasting changes in key proteins in various areas of the brain and spinal cord. This suggests that molecular mapping is a useful tool to study brain and spinal cord reorganization after SCI.
Neuroplasticity is often evoked to explain adaptation and compensation after acute lesions of the Central Nervous System (CNS). In this study, we investigated the modification of 80 genes involved in synaptic plasticity at different times (24 h, 8 and 45 days) from the traumatic spinal cord injury (SCI), adopting a bioinformatic analysis. mRNA expression levels were analyzed in the motor cortex, basal ganglia, cerebellum and in the spinal segments rostral and caudal to the lesion. The main results are: (i) a different gene expression regulation is observed in the Spinal Cord (SC) segments rostral and caudal to the lesion; (ii) long lasting changes in the SC includes the extracellular matrix (ECM) enzymes Timp1, transcription regulators (Egr, Nr4a1), second messenger associated proteins (Gna1, Ywhaq); (iii) long-lasting changes in the Motor Cortex includes transcription regulators (Cebpd), neurotransmitters/neuromodulators and receptors (Cnr1, Gria1, Nos1), growth factors and related receptors (Igf1, Ntf3, Ntrk2), second messenger associated proteins (Mapk1); long lasting changes in Basal Ganglia and Cerebellum include ECM protein (Reln), growth factors (Ngf, Bdnf), transcription regulators (Egr, Cebpd), neurotransmitter receptors (Grin2c). These data suggest the molecular mapping as a useful tool to investigate the brain and SC reorganization after SCI.
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