Article
Biochemistry & Molecular Biology
Eun-Koung An, Wei Zhang, Minseok Kwak, Peter Chang-Whan Lee, Jun-O Jin
Summary: This study found that Astragalus membranaceus polysaccharide (AMP) can activate dendritic and T cells in human blood, suggesting potential applications in regulating human immunity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Guangming Chen, Nan Jiang, Junping Zheng, Haiming Hu, Huabing Yang, Aizhen Lin, Baifei Hu, Hongtao Liu
Summary: In this study, two homogeneous polysaccharides (APS-A1 and APS-B1) were isolated from Astragalus membranaceus and their chemical structures were characterized. APS-A1 had a 1,4-alpha-D-Glcp backbone with a 1,4,6-alpha-D-Glcp branch every ten residues, while APS-B1 was a heteropolysaccharide composed of glucose, galactose, and arabinose. Bioactivity assays showed that both APS-A1 and APS-B1 exhibited potential anti-inflammatory activity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Applied
Yang Cai, Zhenyuan Si, Ying Jiang, Meng Ye, Feng Wang, Xiaobing Yang, Juping Yu, Xiangdong Gao, Wei Liu
Summary: This study investigated the structure-activity relationship of Astragalus membranaceus polysaccharides (APS) using two carbohydrate-active enzymes. The degradation products were divided into different groups based on their molecular weights. The results showed that APS-A1 and APS-G3 had better immunomodulatory activity, while APS-G1 and APS-G2 had weaker activity. Molecular interaction experiments revealed that APS-A1 and APS-G3 could bind to toll-like receptor-4 (TLR-4), while APS-G1 and APS-G2 could not. The branched chains of galactose or arabinogalactooligosaccharide played a crucial role in the immunomodulatory activity of APS.
CARBOHYDRATE POLYMERS
(2023)
Article
Pharmacology & Pharmacy
Qianbo Song, Sau Wan Cheng, Dan Li, Huiyuan Cheng, Yuen Sze Lai, Quanbin Han, Hoi Yan Wu, Pang Chui Shaw, Zhong Zuo
Summary: This study investigated the role of gut microbiota in the hypoglycemic effect of Astragalus membranaceus polysaccharides (AMP) in db/db mice. Treatment with AMP significantly alleviated diabetic symptoms and restored gut microbiota community, leading to increased production of short chain fatty acids (SCFA) in feces. The relative abundance of two intestinal bacteria, Akkermansia and Faecalibaculum, were found to be positively correlated with the hypoglycemic effect of AMP and fecal SCFA production. Furthermore, AMP treatment stimulated the secretion of glucagon-like peptide-1 (GLP-1) and improved intestinal integrity. Mechanistic study revealed that the increased SCFA after AMP treatment could enhance the expression of G protein-coupled receptors 41/43 and tight junction proteins, resulting in the alleviation of diabetic symptoms in db/db mice.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Miaoyin Dong, Jinjuan Li, Delong Yang, Mengfei Li, Jianhe Wei
Summary: Astragalus membranaceus, a well-known traditional herbal medicine, has been used for more than 2000 years and its bioactive compounds have shown various pharmacological effects, such as liver protection, immunomodulation, and anti-cancer activities. This review comprehensively discusses the biosynthesis pathway of flavonoids and triterpenoid saponins in Astragalus membranaceus, as well as the structural features of polysaccharides. The review also summarizes the pharmacological effects of these compounds, providing a foundation for the development of clinical candidate agents. Additionally, potential strategies for heterologous biosynthesis are proposed to improve the industrial production and supply of natural products from Astragalus membranaceus.
Article
Biochemistry & Molecular Biology
Yinfang Pu, Shengjun Wu
Summary: The aim of this study was to investigate the effects of Astragalus membranaceus polysaccharide (AMP) on the growth performance, body composition and non-specific immune index of white shrimps (Litopenaeus vannamei). The results demonstrated that AMP with appropriate dose could promote the growth of shrimps and improve their disease resistance.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Fisheries
Mayada R. Farag, Youssef A. Attia, Fulvia Bovera, Antonino Nizza, Mahmoud Alagawany, Lafi S. Al Solami
Summary: The present study investigated the effects of thallium exposure on behavior, biochemistry, and molecular responses in Oreochromis niloticus fish. It was found that supplementation with Astragalus membranaceus polysaccharides attenuated the detrimental effects of thallium exposure, suggesting its neuroprotective activity.
Article
Veterinary Sciences
Mayada R. Farag, Mahmoud Alagawany, Samah R. Khalil, Eman W. El-Hady, Walaa M. Elhady, Tamer Ahmed Ismail, Carlotta Marini, Alessandro Di Cerbo, Hany M. R. Abdel-Latif
Summary: This study evaluated the immunotoxic effects of thallium (Tl) on Nile tilapia fingerlings and the recovery role of dietary Astragalus membranaceus polysaccharides (ASs). The results showed that Tl exposure had negative effects on hematobiochemical parameters and immune indices of the fish, as well as increased susceptibility to bacterial infection. However, feeding ASs could partially alleviate these effects.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Yingying Qiao, Changzhong Liu, Yongpeng Guo, Wei Zhang, Weibing Guo, Kyselov Oleksandr, Zhixiang Wang
Summary: This study investigated the effects of dietary supplementation of polysaccharides derived from Astragalus membranaceus and Glycyrrhiza uralensis on growth performance, intestinal health, and gut microbiota composition in broilers. The results showed that both polysaccharides improved growth performance, enhanced immune function, and modulated gut microbiota in broilers.
Article
Biochemistry & Molecular Biology
Bangfu Liu, Zhijian Tan
Summary: This study utilized deep eutectic solvents-based aqueous two-phase systems for the extraction and separation of Astragalus membranaceus polysaccharides. The method proved to be efficient, environmentally friendly, and cost-effective.
Article
Biochemistry & Molecular Biology
Xiaoxia Chen, Chun Chen, Xiong Fu
Summary: The study demonstrated that Astragalus membranaceus polysaccharides have hypoglycemic effects in type 2 diabetes mellitus mice by improving glycolipid metabolism disorders and inhibiting intestinal pathogens, protecting the intestinal barrier.
Article
Fisheries
Hany M. R. Abdel-Latif, Hamada A. Ahmed, Mustafa Shukry, Md Reaz Chaklader, Rasha M. Saleh, Mohamed A. Khallaf
Summary: The present study evaluated the impacts of powdered Astragalus membranaceus extract on the growth, physiological responses, and serum immunity of Pangasianodon hypophthalmus juveniles. The results showed that Astragalus membranaceus extract can improve the growth performance and immune function of fish.
Article
Oncology
Shih Ming Tsao, Tz Chin Wu, JiZhen Chen, Feichi Chang, Thomos Tsao
Summary: Astragalus polysaccharide injection (PG2) can normalize the NLR in lung cancer patients receiving immunotherapy and has a potential impact on overall survival.
INTEGRATIVE CANCER THERAPIES
(2021)
Article
Acoustics
Yue Meng, Xiaoyu Sui, Xu Pan, Xinyi Zhang, Huimin Sui, Tao Xu, Honglian Zhang, Tingting Liu, Jicheng Liu, Pengling Ge
Summary: In this study, a green and high-density natural deep eutectic solvent was used for the extraction and purification of Astragalus polysaccharides under ultrasound-assisted conditions. By selecting the best solvent and optimizing the extraction process, the recovery of polysaccharides was significantly increased, simplifying the purification process and reducing impurities.
ULTRASONICS SONOCHEMISTRY
(2023)
Article
Oncology
Zixiong Zhou, Xijie Yu, Yuqing Chen, Xiaodan Tan, Wenwen Liu, Wenxi Hua, Lihong Chen, Wenmin Zhang
Summary: This study evaluated the expression of B7-H3 protein in HCC patients and its correlation with prognosis, as well as the therapeutic potential of targeting B7-H3 in HCC. It was found that overexpression of B7-H3 in tumor cells was associated with poor prognosis in HCC patients. Inhibiting B7-H3 increased immune activity and tumor cell killing mediated by T cells. Therefore, B7-H3 serves as a key negative regulator in tumor immunity and could be a promising target for immunotherapies in HCC treatment.
CLINICAL & TRANSLATIONAL ONCOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Hae-Bin Park, Juyoung Hwang, Seong-Min Lim, Wei Zhang, Jun-O Jin
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2020)
Review
Biochemistry & Molecular Biology
Jun-O Jin, Gyurin Kim, Juyoung Hwang, Kyung Ho Han, Minseok Kwak, Peter C. W. Lee
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER
(2020)
Article
Biochemistry & Molecular Biology
Juyoung Hwang, Jun-O Jin
Article
Oncology
Wei Zhang, Seong-Min Lim, Juyoung Hwang, Srinivasan Ramalingam, Myunghee Kim, Jun-O Jin
Summary: MPLA enhances anti-cancer immunity mediated by anti-PD-L1 antibodies by activating pDCs and increasing IFN-α production.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Seong-Min Lim, Juyoung Hwang, Hae-Bin Park, Chan Ho Park, Jun-O Jin
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Wei Zhang, Hae-Bin Park, Dhananjay Yadav, Juyoung Hwang, Eun-Koung An, Hee-Yun Eom, So-Jung Kim, Minseok Kwak, Peter Chang-Whan Lee, Jun-O Jin
Summary: The study revealed that Ecklonia cava fucoidan has the strongest impact on activating human dendritic cells, upregulating costimulatory molecules, major histocompatibility complex, and proinflammatory cytokines production. It also showed the most significant induction of T cell proliferation and IFN-gamma production compared to other fucoidans, suggesting it could be a suitable candidate for enhancing immune activation in humans.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Medicinal
Hae-Bin Park, Juyoung Hwang, Wei Zhang, Seulgi Go, Jihoe Kim, Inho Choi, SangGuan You, Jun-O Jin
Article
Engineering, Environmental
Jeong-Hun Kim, Yu-Ri Oh, Juyoung Hwang, Jaeryeon Kang, Hyeri Kim, Young-Ah Jang, Seung-Soo Lee, Sung Yeon Hwang, Jeyoung Park, Gyeong Tae Eom
Summary: Waste-cooking oil (WCO), defined as vegetable oil used to fry food at high temperatures, generates 41-67 million tons annually globally. Without proper treatment, it causes serious environmental issues, but through biotechnology, WCO can be recycled to produce high value-added compounds, extending its applications to the plastics industry.
Article
Chemistry, Multidisciplinary
Sangpil Kim, Batakrishna Jana, Eun Min Go, Ji Eun Lee, Seongeon Jin, Eun-Koung An, Juyoung Hwang, Youjung Sim, Sehee Son, Dohyun Kim, Chaekyu Kim, Jun-O Jin, Sang Kyu Kwak, Ja-Hyoung Ryu
Summary: The study developed an intramitochondrial polymerization-induced self-assembly system for regulating the fate of cancer cells, inducing cell necroptosis by increasing ROS levels. This in situ polymerization system shows great potential for cancer treatment, including drug-resistant cancers.
Article
Biochemistry & Molecular Biology
Wei Zhang, Eun-Koung An, Hae-Bin Park, Juyoung Hwang, Yadav Dhananjay, So-Jung Kim, Hee-Yun Eom, Tatsuya Oda, Minseok Kwak, Peter Chang-Whan Lee, Jun-O Jin
Summary: The study found that Ecklonia cava fucoidan (ECF) had the strongest effect on activating NK cells in mice, increasing the number of NK cells in the spleen and promoting NK cell activation by affecting dendritic cells. ECF treatment also effectively prevented infiltration of cancer cells in the lungs of mice, demonstrating its potential to enhance NK cell-mediated anti-cancer immunity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Wei Zhang, Juyoung Hwang, Dhananjay Yadav, Eun-Koung An, Minseok Kwak, Peter Chang-Whan Lee, Jun-O Jin
Summary: Intranasal administration of Ecklonia cava-extracted fucoidan (ECF) was found to induce anti-cancer immunity in the lung by promoting the activation of dendritic cells, natural killer cells, and T cells. Furthermore, ECF enhanced the anti-cancer activities against melanoma and carcinoma tumor growth in the lungs, showing potential as a mucosal adjuvant for enhancing immunotherapeutic effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Wei Zhang, Eun-Koung An, Juyoung Hwang, Jun-O Jin
Summary: The study found that LPS can directly stimulate pDCs via the TLR4/MD2 pathway and promote the activation of pDCs and cDCs. cDCs may contribute to the enhancement of IFN-alpha production in pDCs.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Oncology
Juyoung Hwang, Wei Zhang, Hae-Bin Park, Dhananjay Yadav, Yong Hyun Jeon, Jun-O Jin
Summary: A hybrid nanoparticle incorporating a thermal responsive property was synthesized in this study, showing potential anticancer and antimetastatic effects against cancer and its recurrence and metastasis through a combination of photothermal therapy and immunotherapy.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2021)
Article
Engineering, Biomedical
Haejoo Kim, Wei Zhang, Juyoung Hwang, Eun-Koung An, Yeol Kyo Choi, Eunyoung Moon, Mark Loznik, Yang Hoon Huh, Andreas Herrmann, Minseok Kwak, Jun-O Jin
Summary: The study engineered a CpG oligodeoxyribonucleotide with multiple hydrophobic moieties, resulting in a self-assembled CpG nanostructure for enhancing anti-HIV immunity. The treatment activated plasmacytoid dendritic cells and natural killer cells to produce interferons, effectively suppressing HIV-1 spread. Additionally, the nanoimmunostimulant reversed HIV-1 latency, making it a potential candidate for clinical studies on viral infection.
Article
Biochemistry & Molecular Biology
Wei-Han Hui, Yen-Lin Chen, Shu-Wei Chang
Summary: Crosslinking affects the mechanical behavior and tissue metabolism of collagen-based tissues. Aging and diabetes alter the crosslinking type and density, thus changing tissue properties. High-connectivity enzymatic crosslinking and advanced-glycation end-products result in uniform deformation under daily activity, while low-connectivity enzymatic crosslinking does not. High-connectivity model displays more sliding, while AGEs induce instability in structures near the binding sites.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yao-Bin Ma, Xiao-Qiu Zhou, Wei-Dan Jiang, Pei Wu, Yang Liu, Shu-Wei Li, Ling Tang, Lu Zhang, Hai-Feng Mi, Lin Feng
Summary: Tea polyphenols (TPs) are an inexpensive feed additive that can protect the gill function of fish against Flavobacterium columnare (F. columnare) through various mechanisms, including suppression of oxidative stress, apoptosis, and inflammation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yan-Jia Guo, Jing-Ru Pang, Yu Zhang, Zhong-Rui Li, Xiao-Lin Zi, Hong-Min Liu, Ning Wang, Li-Juan Zhao, Ya Gao, Bo Wang, Piet Herdewijn, Cheng-Yun Jin, Ying Liu, Yi-Chao Zheng
Summary: This study identifies the conjugation of NEDD8 to LSD1 and its impact on GC cells, revealing the significance of LSD1 neddylation in GC.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Geum-Jae Jeong, Fazlurrahman Khan, Nazia Tabassum, Young-Mog Kim
Summary: Vibrio species are motile gram-negative bacteria commonly found in aquatic environments. They can adapt to environmental fluctuations and develop protective mechanisms against host immune systems. Different types of sigma and anti-sigma factors play important roles in regulating gene expression and virulence in Vibrio species.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Zahra Mohammadbagheri, Behrooz Movahedi, Shaghayegh Saeedi, Abbas Rahmati
Summary: In this study, a reusable biobased composite hydrogel was successfully synthesized and used for the removal of Tl(I) from aqueous solutions. The hydrogel exhibited high adsorption capacity and thermal stability, and the adsorption process was found to be thermodynamically spontaneous. The results demonstrated that the hydrogel can be reused multiple times without significant loss of its adsorption capacity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Iqra Batool, Muhammad Imran, Ayesha Anwar, Farhan Ahmed Khan, Afrah E. Mohammed, Ashwag Shami, Hafiz Iqbal
Summary: Enzyme immobilization on ZnO/SnO2 and ZnO/SnO2/SA nanocomposites was studied, and the catalytic and biochemical characteristics of the immobilized enzyme were investigated. The results showed that ZnO/SnO2 and ZnO/SnO2/SA nanostructures are suitable carriers for enzyme immobilization.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Elias Mosaffa, Dhruvi Patel, Nasim Amiri Ramsheh, Rishikumar Indravadan Patel, Atanu Banerjee, Hossein Ghafuri
Summary: In this study, a unique 3D bead structure of adsorbent, composed of bacterial cellulose microfilaments reinforced chitosan adorned with melamine 2D plates, was synthesized for anionic dye removal. The adsorbent showed high specific surface area and abundant functional groups, resulting in exceptional adsorption capacity. The characteristics and optimal conditions of the adsorbent were assessed and analyzed.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Carolina Gismene, Jorge Enrique Hernandez Gonzalez, Marilia de Freitas Calmon, Andrey Fabricio Ziem Nascimento, Angela Rocio Nino Santisteban, Felipe Antunes Calil, Alana Della Torre da Silva, Paula Rahal, Rejane Maira Goes, Raghuvir Krishnaswamy Arni, Ricardo Barros Mariutti
Summary: Mammaliicoccus sciuri, a bacterium of clinical and veterinary relevance, expresses the virulence factor Exfoliative Toxin C (ExhC), which binds specifically to desmoglein-1 (Dsg1) and plays a role in tissue integrity. This study investigates the necrotic activity of ExhC and identifies a variant lacking necrotic potential but retaining enzymatic activity through mutagenesis experiments and crystal structure determination.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Bibhas K. Bhunia, Ashutosh Bandyopadhyay, Souradeep Dey, Biman B. Mandal
Summary: Intervertebral disc degeneration is a major cause of chronic low back pain and disability. Researchers have developed a bioactive silk-based hybrid hydrogel system functionalized with decellularized human Wharton's jelly extracellular matrix as bioactive cues. The hydrogels mimic the rheological and mechanical properties of the native nucleus pulposus tissue and support cell viability, proliferation, and tissue maturation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Qun Zhang, Yonggan Yan, Zhao Li, Jing Du, Kai Zhang, Liguo Zhang, Ting Wang, Alberto Bianco, Shaohua Ge, Baojin Ma
Summary: A new crosslinking strategy has been devised to construct injectable sodium alginate hydrogels, which can be regulated for injectability and have great potential in biomedical applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Seong Eun Hong, Ji-Soo Lee, Hyeon Gyu Lee
Summary: The potential of multi-layer nanoemulsions for improving the cellular uptake, antioxidant activity, and in vitro bioaccessibility of alpha-tocopherol was examined. The results showed that the multi-layer nanoemulsions significantly enhanced the cellular uptake and antioxidant activity of alpha-tocopherol compared to free coumarin 6. Furthermore, the use of chitosan and dextran sulfate as wall materials in the multi-layer nanoemulsions led to even higher cellular uptake, in vitro bioaccessibility, and antioxidant activity of alpha-tocopherol.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jingjing Yu, Yanhui Zhang, Ruoning Zhang, Yanxiang Gao, Like Mao
Summary: High internal phase emulsions (HIPEs) based on beeswax (BW) oleogels and octenyl succinic acid starch (OSA starch) were prepared by a simple one-step method. The results showed that oleogelation improved the stability and encapsulation capability of the HIPEs, which is significant for the development of novel healthy food.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Bi-Feng Yao, Xiu-Ju Luo, Jun Peng
Summary: The balanced and continuous fission and fusion of mitochondria, regulated by OPA1, are important for maintaining mitochondrial dynamics in cardiovascular disorders. Understanding the role of OPA1 in mitochondrial fusion can provide insights into the pathogenesis of cardiovascular diseases.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Gokul Paramasivam, Anandhavelu Sanmugam, Vishnu Vardhan Palem, Murugan Sevanan, Ananda Babu Sairam, Nachiappan Nachiappan, Buhyun Youn, Jung Sub Lee, Muthuchamy Nallal, Kang Hyun Park
Summary: Nanomaterials are being used to deliver therapeutic agents in living systems. Different nanotechnological applications such as immunosensors, immune assays, and drug delivery are being implemented using nanotechnology. Genomics plays a crucial role in selecting appropriate drugs for personalized cancer therapy. It helps in analyzing gene and protein expression, disease classification, and designing disease models. Delivering drugs to specific areas using nanoparticles is a challenging issue.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jing Li, Wei Wang, Han Wu, Feng Peng, Hui Gao, Ying Guan
Summary: In this study, CMH-based CPF films were prepared and their thermal and barrier properties were successfully regulated. The tensile strength and water vapor barrier property of CPF films were significantly improved, making them promising for food packaging applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)