期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 180, 期 -, 页码 272-285出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2021.03.056
关键词
Plasmodium falciparum; Hsp110; PfHsp70-z; Allostery; Linker; Chaperone
资金
- National Research Foundation Doctoral Scholarship
- African-German Network of Excellence in Science junior researcher grant
- Georg Foster research fellowship by the Alexander von Humboldt Foundation, Germany
- Department of Science and Technology/National Research Foundation (NRF) of South Africa [75464]
- NRF [92598]
The study reveals the distinct functional characteristics of two chaperones in Plasmodium falciparum, with PfHsp70-z's unique linker segment impacting its global conformation and thermal stability, regulating functions such as client protein binding and ATPase activities.
Plasmodium falciparum expresses two essential cytosol localised chaperones; PfHsp70-1 and PfHsp70-z. PfHsp70-z (Hsp110 homologue) is thought to facilitate nucleotide exchange function of PfHsp70-1. PfHsp70-1 is a refoldase, while PfHsp70-z is restricted to holdase chaperone function. The structural features delineating functional specialisation of these chaperones remain unknown. Notably, PfHsp70-z possesses a unique linker segment which could account for its distinct functions. Using recombinant forms of PfHsp70-1, PfHsp70-z and E. coli Hsp70 (DnaK) as well as their linker switch mutant forms, we explored the effects of the linker mutations by conducting several assays such as circular dichroism, intrinsic and extrinsic fluorescence coupled to biochemical and in cellular analyses. Our findings demonstrate that the linker of PfHsp70-z modulates global conformation of the chaperone, regulating several functions such as client protein binding, chaperone-and ATPase activities. In addition, as opposed to the flexible linker of PfHsp70-1, the PfHsp70-z linker is rigid, thus regulating its notable thermal stability, making it an effective stress buffer. Our findings suggest a crucial role for the linker in streamlining the functions of these two chaperones. The findings further explain how these distinct chaperones cooperate to ensure survival of P. falciparum particularly under the stressful human host environment. (c) 2021 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http:// creativecommons.org/licenses/by-nc-nd/4.0/).
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