Antibacterial activity and mechanism of the graphene oxide (rGO)- modified TiO2 catalyst against Enterobacter hormaechei

The antibacterial activity and mechanism of reduced graphene oxide (rGO)-modified TiO2 were assessed against Enterobacter hormaechei in this study. The rGO-modified TiO2 exhibited an enhanced antibacterial activity against E. hormaechei under UV-Visible light irradiation compared with individual TiO2 or rGO alone. Its effectiveness owed to the synergistic effect of the improved adsorption and the lower recombination of photo-induced electron-hole pairs. The hybrid photocatalyst improved charge separation and chemical binding activity that contributed to accelerated antibacterial process. The flow cytometry results showed that the hybrid photocatalyst reduced the survival ratio of E. hormaechei to about 10% with 90 min under visible light illumination. The XPS spectra and SEM micrographs indicated a direct physical contact and disruptive interaction between the bacteria and the rGO-TiO2 catalyst, mostly on rGO, resulting in oxidizing a vital cellular structure. EPR measurement revealed that hydroxyl radicals were the dominant active species that can cause the peroxidation of lipids in the outer cell wall and the damage of cell organelles and components inside the bacteria, which is the key mechanism for this antibacterial process. Overall, this study indicated that rGO-modified TiO2 nanocomposite has great potential as an economical, environmentally friendly and easily available antibacterial material for antibacterial.

Automated summary

The study demonstrated that rGO-modified TiO2 nanocomposite exhibited enhanced antibacterial activity against Enterobacter hormaechei under UV-Visible light due to the improved adsorption and the reduced recombination of photo-induced electron-hole pairs. The hybrid photocatalyst improved charge separation and chemical binding activity, contributing to the accelerated antibacterial process when compared with individual TiO2 or rGO alone.