期刊
INORGANIC CHEMISTRY
卷 60, 期 19, 页码 14515-14519出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.inorgchem.1c01863
关键词
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资金
- National Key R&D Program of China [2017YFA0505400, 2020YFA0710700]
- National Science Foundation of China [21877103, 52021002, 22177109]
- Fundamental Research Funds for the Central Universities [WK3450000007]
- Users with Excellence Program of Hefei Science Center, Chinese Academy of Science (CAS) [2020HSC-UE016]
A protein-based nanozyme has been constructed by encapsulating hemin into the nanocavity of recombinant human heavy chain ferritin, which possesses high peroxidase catalytic activity and high tolerance to harsh environmental conditions, serving as a colorimetric probe for tumor cell detection.
Nanozyme is a class of artificial materials that possess enzyme-like activities and can overcome limitations of natural enzymes. However, controllability of the active sites, uniformity of the particles, and dispersion in the physiological media are still challenging for nanomaterial-based nanozymes. In this work, a protein-based nanozyme has been constructed by the encapsulation of hemin into the nanocavity of a recombinant human heavy chain ferritin (Ftn), generating a monodispersed peroxidase-mimetic nanozyme (hemin@Ftn). Hemin@Ftn possesses high peroxidase catalytic activity and high tolerance to the harsh environmental conditions, such as high temperature and chemical denaturant. Remarkably, hemin@Ftn can act as a colorimetric probe for the detection of tumor cells because it can selectively catalyze reactions in tumor cells. This protein-based nanozyme bridges the gap between natural enzymes and nanomaterial-based nanozymes by the incorporation of a catalytically active prosthetic group into a highly stable Ftn.
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