期刊
FISH & SHELLFISH IMMUNOLOGY
卷 48, 期 -, 页码 185-189出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2015.11.025
关键词
Fenneropenaeus chinensis; Haemocyanin; Double antibody sandwich ELISA; White spot syndrome virus; Vibrio harveyi
资金
- NBRPC [2012CB114405]
- Key Technology R&D Program of China [2012BAD17B01]
- Special Fund for Agro-scientific Research in the Public Interest [201103034]
- Taishan Scholar Program of Shandong Province
Haemocyanin (Hc) is frequently reported to vary significantly by physiological status and environmental stress in Crustaceans. In this paper, the shrimp Fenneropenaeus chinensis was infected with different concentrations of white spot syndrome virus (WSSV) and Vibrio harveyi. Then, the variation of Hc and total protein content of the haemolymph (TPCH) were investigated using the established double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA) and Coomassie brilliant blue method, respectively. The results showed that the Hc content peaked at 12 h post-infection (PI) in the 10(-2), 10(-4) and 10(-6) viral supernatant (VS) groups, and the maximum was 93.03 +/- 2.55 mg ml(-1), 77.57 +/- 6.02 mg ml(-1) and 70.25 +/- 3.96 mg ml(-1), respectively. TPCH reached the maximum of 108.18 +/- 1.36 mg ml(-1) and 103.49 +/- 1.33 mg ml(-1) at 12 h PI in the 10(-2) and 10(-4) VS groups, respectively. The maximum was 96.94 +/- 1.06 mg ml(-1) at 24 h PI in the 10(-6) VS group. In the V. harveyi infection groups, the Hc content reached a maximum of 87.97 +/- 4.39 mg ml(-1) at 36 h PI in the 106 CFU ml(-1) group, 73.74 +/- 4.38 mg ml(-1) and 72.47 +/- 2.09 mg ml(-1) at 12 h PI in the 10(7) and 10(8) CFU ml(-1) groups, respectively. TPCH reached a maximum of 111.16 +/- 0.86 mg ml(-1) at 36 h PI in the 10(6) CFU ml(-1) group, 100.41 +/- 0.51 mg ml(-1) and 101.94 +/- 0.47 mg ml(-1) at 12 h PI in the 10(7) and 10(8) CFU ml(-1) groups, respectively. These data showed that both Hc content and TPCH varied as the same extent after infection. The up-regulation of the Hc content at 6-36 h PI might be a reference threshold for shrimp infection. (C) 2015 Elsevier Ltd. All rights reserved.
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