Article
Fisheries
Yan Zhao, Qiang Luo, Wansu Wang, Shang Geng, Yuena Sun, Tianjun Xu
Summary: This study identified METTL16 in miiuy croaker and found that its expression was increased after poly(I:C) stimulation, suggesting its involvement in antiviral immunity. Functional experiments showed that mmiMETTL16 could inhibit immune response caused by viral stimulation. Overall, the study on mmiMETTL16 will contribute to understanding the role of METTL16 in the teleost immune system and the potential mechanisms of the m6A regulation network.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Article
Fisheries
Xing Lv, Qiang Luo, Shiying Xin, Weiwei Zheng, Tianjun Xu, Yuena Sun
Summary: In recent years, there has been an increasing focus on the study of circular RNAs (circRNAs) in noncoding RNAs. While circRNAs are abundant in mammalian studies, research in lower vertebrates has been lacking. In this study, a circRNA called circPlce1 was found to be related to the innate immune response in Miichthys miiuy (miiuy croaker). Experimental results showed that circPlce1 could promote the production of antiviral genes and inflammatory response. It was also observed that circPlce1 can promote cell proliferation and improve cell viability. The study provides a foundation for understanding the biological function of circRNAs in the innate immune response in teleost fish.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Shang Geng, Tianjun Xu, Yuena Sun
Summary: In this study, we identified 27 ChemR genes in miiuy croaker and analyzed their characteristics, including gene structure, evolutionary mechanism, and expression levels upon bacterial infection. The results showed that these genes underwent tandem and segmental duplication events during evolution, and teleost fishes' ChemRs may have a higher evolutionary dynamic compared to vertebrates. Additionally, 21 positively selected codons were detected in vertebrate ChemRs under Model 8, and certain genes were significantly up-regulated upon Vibrio harveyi infection, providing valuable information for further functional analysis.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Fisheries
Qing Chu, Jingjing Han, Lingping Sun, Junxia Cui, Tianjun Xu
Summary: MDA5, a crucial member of RIG-I-like receptors, plays a significant role in antiviral immune response. MicroRNAs have been discovered to regulate the RLR signaling pathway, with miR-203 directly negatively regulating MDA5 in miiuy croaker. This study sheds light on miRNA-mediated regulation of immune responses and cytokine suppression in teleost fish, offering new insights for mammalian immune response studies.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Fisheries
Shiying Xin, Xing Lv, Linchao Wang, Wei Wei Zheng, Tianjun Xu, Yuena Sun
Summary: With the in-depth study of circRNA, its important role in mammals, such as cell proliferation, apoptosis, invasion, development, and disease state, has been demonstrated. However, the regulatory mechanism of circRNA in lower vertebrates remains unclear. In this study, a new circRNA named circRara was identified and its positive regulatory effect on the antiviral and antibacterial response, cell proliferation, and activity in miiuy croaker was confirmed.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Baolan Cao, Yan Zhao, Qiang Luo, Ya Chen, Tianjun Xu, Yuena Sun
Summary: In this study, Vclb was found to inhibit the NF-KB signaling pathway, and its inhibitory effect was enhanced by LPS induction. Furthermore, Vclb inhibits NF-KB activation by targeting MyD88, thereby suppressing the production of inflammatory cytokines. Mechanistically, Vclb inhibits the NF-KB signaling pathway by targeting MyD88 ubiquitin-proteasome pathway.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Liyuan Yang, Weiwei Zheng, Shiying Xin, Xing Lv, Yuena Sun, Tianjun Xu
Summary: This study finds that miR-122 can promote the activation of NF-kappa B pathway by regulating the expression of I kappa B alpha, thus playing an important role in the immune response of teleost fish.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Qiang Luo, Xuefeng Zhou, Xing Lv, Weiwei Zheng, Shang Geng, Tianjun Xu, Yuena Sun
Summary: Alternative splicing is an important mechanism for controlling gene expression in eukaryotes. This study identified three different splicing isoforms in the ferritin gene fthl27 of Miichthys miiuy and found that they showed an upregulation trend after stimulation. These isoforms may have different regulatory effects on inflammatory factors and antiviral immune factors, playing a significant role in the innate immune response of fish.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Liyuan Yang, Weiwei Zheng, Xing Lv, Shiying Xin, Yuena Sun, Tianjun Xu
Summary: In this study, miR-144 was investigated as a regulator of I kappa B alpha, promoting the activation of the NF-kappa B signaling pathway. The findings suggest that miR-144 can promote p65 activation by inhibiting I kappa B alpha, thus affecting the NF-kappa B signaling pathway.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Fisheries
Jiajia Pan, Weiwei Zheng, Yuena Sun, Tianjun Xu
Summary: By studying the miiuy croaker, a lncRNA named LTCONS5539 was found to play a critical regulatory role in its antiviral and antibacterial response through modulating TRAF6-mediated immune responses.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Virology
Junxia Cui, Weiwei Zheng, Tianjun Xu, Yuena Sun
Summary: Long noncoding RNAs (lncRNAs) play an important role in the immune response of fish to pathogen infections. This study found that MIR122HG can negatively regulate the antiviral immune response in miiuy croaker and prevent excessive immune response. Furthermore, MIR122HG promotes viral replication.
Article
Biochemistry & Molecular Biology
Takahisa Kouwaki, Tasuku Nishimura, Guanming Wang, Reiko Nakagawa, Hiroyuki Oshiumi
Summary: Post-translational modification of LGP2 fine-tunes antiviral innate immune responses by promoting late-phase expression of type I interferons, while attenuating other antiviral proteins.
Article
Fisheries
Wenjing Dong, Wenya Gao, Xiaolong Yan, Yuena Sun, Tianjun Xu
Summary: The innate immune system is the first line of defense against pathogens, while microRNA miR-132 plays a crucial role in regulating immune responses, specifically by targeting IL-1 beta to prevent excessive inflammation.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Cell Biology
Junyi Song, Chuanyang Liu, Baoshan Li, Liangcheng Liu, Ling Zeng, Zonghuang Ye, Ting Mao, Wenjian Wu, Biru Hu
Summary: Reflectin proteins are natural copolymers that manipulate the structural coloration of cells. This study found differences in cellular localization among reflectin proteins, suggesting a possible evolutionary order. Additionally, reflectin proteins interact with the cytoskeleton, indicating its important role in their organization and transportation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Environmental Sciences
Wang-Yu Zhu, Yu-Mei Wang, Shi-Kun Suo, Shuo-Lei Zheng, Bin Wang
Summary: In this study, swim bladder hydrolysate with the highest ACEi activity was prepared from miiuy croaker using an in vitro gastrointestinal digestion method. Seven novel ACEi peptides were isolated from the hydrolysate, with SHGEY and SPYGF showing remarkable ACEi activity. Molecular docking experiments revealed that the ACEi activity of SHGEY and SPYGF was mainly attributed to their effective interaction with the ACEi active sites through hydrophobic interaction, electrostatic force, and hydrogen bonding. Moreover, SHGEY and SPYGF promoted NO production and decreased ET-1 secretion in HUVECs, and protected HUVECs from H2O2 damage.
FRONTIERS IN MARINE SCIENCE
(2022)
