期刊
FISH & SHELLFISH IMMUNOLOGY
卷 50, 期 -, 页码 109-116出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2015.12.003
关键词
Apoptosis signal-regulating kinase 1; Litopenaeus vannamei; White spot syndrome virus; Apoptosis
资金
- National Natural Science Foundation of China [31472301, U1131002]
- Guangdong Natural Science Funds for Distinguished Young Scholar [2015A030306030]
- National Basic Research Program of China (973 program) [2012CB114401]
- National Key Technology R&D program of China [2011BAD13B10]
- National Science Fund for Young Scholars of China [31202017]
Apoptosis signal-regulating kinase 1 (ASK1), a mitogen-activated protein kinase kinase kinase, is crucial in various cellular responses. In the present study, we identified and characterized an ASK1 homolog from Litopenaeus vannamei (LvASK1). The full-length cDNA of LvASK1 was 5400 bp long, with an open reading frame encoding a putative 1420 amino acid protein. LvASK1 was highly expressed in muscle, hemocyte, eyestalk and heart. Real-time RT-PCR analysis showed that the expression of the LvASK1 was upregulated during the white spot syndrome virus (WSSV) challenge. The knocked-down expression of LvASK1 by RNA interference significantly reduced the apoptotic ratio of the hemocytes collected from WSSV-infected L. vannamei. Furthermore, the down-regulation of LvASK1 also decreased the cumulative mortality of WSSV-infected L. vannamei. These results suggested that down-regulation of LvASK1 decreased the apoptotic rate of hemocytes in WSSV-infected shrimp, and that it could contribute to the reduction of cumulative mortality in WSSV-infected L. vannamei. (C) 2016 Elsevier Ltd. All rights reserved.
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