Silicon-nanoparticle Mediated Changes in Seed Germination and Vigor Index of Marigold (Calendula Officinalis L.) Compared to Silicate Under PEG-induced Drought Stress

The current study explores the potential impact of seed priming with silicon nanoparticles (SiNPs, 0, 100, 200, 500 mg/L) and silicate (Si, 0, 1, 1.5, 2 mg/L) under polyethylene glycol (PEG)-induced drought stress (0, -0.5, -1, and -1.5 MPa, respectively, control, mild, moderate and severe drought stress) on germination features and vigor index of marigold (Calendula officinalis L.). The study was performed in a factorial experiment based on completely randomized design (CRD) with four replications. Based on scanning electron microscopy (SEM) analysis, application of SiNPs at high level in seeds subjected to the severe drought stress clearly revealed the greater deposition of SiNPs on root cell walls compared to the mild or moderate drought stress. The cumulative germination in response to various concentrations of Si and SiNPs showed different germination patterns under drought stress intensities. Two-way interaction of SiNPs and drought stress significantly (P < 0.01) affected the mean germination time only, however, interaction of Si and drought stress significantly influenced germination rate (P < 0.05) and germination index (P < 0.01). The vigor index (VI) based on seedlings length (SL) and dry weight (DW) at 14 days after planting were significantly higher in Si- and SiNPs-treated seeds at 2 and 500 mg/L compared to the other treatments, respectively. In both Si and SiNPs treatments, VI-SL and VI-DW showed positive and significant correlation (r(0.01) = 0.737 and r(0.01) = 0.859) and (r(0.01) = 0.639 and r(0.01) = 0.821) with germination percentage under drought stress, respectively. These findings will open the opportunity of using Si and SiNPs as priming agents to enhance seed germination and improve seedling growth under water deficit stress.

Automated summary

The study explored the potential impact of seed priming with silicon nanoparticles and silicate under PEG-induced drought stress on germination features and vigor index of marigold. The application of SiNPs at high level under severe drought stress showed greater deposition on root cell walls. Si and SiNPs treatments significantly influenced germination patterns and vigor index, with high levels leading to better outcomes under water deficit stress.