Article
Chemistry, Analytical
Weijie Wu, Xinyi Liu, Mengfei Shen, Lisong Shen, Xing Ke, Daxiang Cui, Wanwan Li
Summary: A novel multicolor quantum dot nanobead-based FLISA platform has been established for highly sensitive multiplexed detection of lung cancer tumor markers. The use of multicolor QDNBs as fluorescent labels enables high detection sensitivity and construction of multiplexed fluorescent biosensors, showing potential for clinical applications.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Hongxin Ren, Guowen Li, Xin Yue, Xiujin Chen, Cunzheng Zhang, Zhouping Wang, Chifang Peng
Summary: Nanomaterial based indicator displacement assays using fluorescent metal nanoclusters as receptors have been developed. In this study, GSH/CD-AuNCs were used as receptors and kaempferol/Al3+ complex as an indicator to fabricate a new nanomaterial based IDA. The fluorescence of GSH/CD-AuNCs was quenched by the KAE/Al3+ complex, but could be restored in the presence of chlorothalonil due to its higher affinity with beta-CD. Based on this principle, a novel fluorescent turn-on IDA-LFA for chlorothalonil was successfully constructed. This method has the advantages of rapid detection and high sensitivity.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Danial Gholamin, Pari Karami, Yasamin Pahlavan, Mohammad Johari-Ahar
Summary: A sandwich-type photoelectrochemical immunosensor based on gold-coated tin selenide quantum dots (Au-SnSe QDs) was developed to detect carbohydrate antigen 19-9 (CA19-9). The sensor showed significant photoelectrochemical response for the presence of CA19-9 antigen in serum samples, with a LOD of 0.0011 U mL(-1) and a dynamic range of 0.005-100 U mL(-1). The recovery of CA19-9 determination from serum samples was 101-113%.
Article
Engineering, Multidisciplinary
Yuting Shang, Shuzhen Cai, Qinghua Ye, Qingping Wu, Yanna Shao, Xiaoying Qu, Xinran Xiang, Baoqing Zhou, Yu Ding, Moutong Chen, Liang Xue, Honghui Zhu, Jumei Zhang
Summary: In this study, a novel QBs-labelled LFIAS combined with SD-LAMP was developed for rapid and sensitive detection of Salmonella Typhimurium. The QBs served as fluorescence reporters, providing good detection efficiency. The SD probe in LAMP improved the specificity of the method. The detection range of the strip was 102-108 CFU mL-1, and the visual limit of detection was 103 CFU mL-1.
Article
Chemistry, Analytical
Sung Eun Seo, Eunsu Ryu, Jinyeong Kim, Chan Jae Shin, Oh Seok Kwon
Summary: The rapid and sensitive detection of SARS-CoV-2 has become a priority for public health. In this study, a new method was proposed to improve the performance of the lateral flow assay sensor by concentrating fluorescent organic molecules. This approach showed high sensitivity, fast detection, and outperformed commercial test kits in clinical tests.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Xijuan Chao, Dezhi Yao, Yongmei Qi, Cong Yuan, Dejun Huang
Summary: A selective light-up fluorescent sensor DCM-ML was successfully developed for quantification of HSA, exhibiting good stability and strong fluorescence enhancement. The sensor showed a linear relationship with HSA concentration and a detection limit of 0.25 mg/mL, suggesting great potential in analytical chemistry and clinical medicine.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Applied
Yu-Nan Lu, Yanke Shan, Huachuan Huang, Lin Zhu, Baojie Li, Shouyu Wang, Fei Liu
Summary: This work proposes a sensitive and quantitative on-site detecting solution for mycotoxins, which can be quickly completed on site through immunochromatography test strips, with high accuracy and good selectivity.
Article
Food Science & Technology
Han Wang, Yu Zheng, Hong-Lin Ren, Yi-Ran Xiao, Cong Wang, Jiang Chang, Yu-Xi Guo, Pan Hu, Yan-Song Li, Zeng-Shan Liu, Shi-Ying Lu
Summary: A lateral flow immunochromatographic strip targeting on virulence gene ail was developed using recombinase polymerase amplification and quantum dot nanobeads for rapid detection of pathogenic Yersinia enterocolitica in meat samples with high sensitivity and accuracy.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Chemistry, Analytical
Jinjie Li, Yanbing Lv, Ning Li, Ruili Wu, Jiangnan Li, Jian You, Huaibin Shen, Xia Chen, Lin Song Li
Summary: A dual protection strategy utilizing PVP winding and SiO2 coating was developed to create QBs@SiO2-COOH nanobeads for rapid LFIA with improved sensitivity and stability. This novel nanosensor allows rapid, stable, and sensitive detection of biomarkers for in vitro diagnostics, showing excellent reproducibility and stability over time.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Pinghua Ling, Xinyu Sun, Xianping Gao, Linyu Wang, Pei Yang, Feng Gao
Summary: In this paper, a novel label-free and extensibility electrochemical sensing platform based on porphyrin-ionic liquid (Por-IL) aggregate for the detection of antibody is described. The Por-IL exhibited high singlet oxygen (O-1(2)) quantum yields and electrocatalytic activity toward hydrogen peroxide (H2O2). In the presence of antibody, the electrochemical signal was generated by the catalysis of water and O-1(2) by the antibody. This immunosensor platform has the potential for medical diagnosis.
Article
Chemistry, Analytical
Tamara Guerrero-Esteban, Cristina Gutierrez-Sanchez, Tania Garcia-Mendiola, Monica Revenga-Parra, Felix Pariente, Encarnacion Lorenzo
Summary: A disposable electrochemiluminescent immunosensor using nitrogen-rich carbon nanodots was developed for efficient detection of the breast cancer biomarker HER2, showing a linear response to HER2 concentration and a detection limit of 20.4 pg mL-1. The biosensor demonstrated good stability and applicability in analyzing human serum samples, indicating great potential for early breast cancer diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Siyu Chen, Shu-Na Yun, Yujiao Liu, Ruijin Yu, Qin Tu, Jinyi Wang, Mao-Sen Yuan
Summary: The abuse of pesticides has resulted in the presence of residues in soil and drinking water, posing risks to the food chain and human health. There is an urgent need for monitoring and developing detection methods for pesticide residues. This study introduces a one-pot prepared CdS fluorescent quantum dots (QDs) and explores their application in detecting organic pesticides. The QDs show sensitive and selective detection of three different pesticides, and a smartphone is utilized for qualitative and quantitative analysis of one of the pesticides.
Article
Biophysics
Bing Liu, Shiya Zheng, Hanyu Tang, Qian Liu, Haitao Li, Bingbing Gao, Xiangwei Zhao, Fei Sun
Summary: A highly sensitive method for the analysis of free testosterone (FT) was developed using magnetic nanobeads (MNBs) and gap-enhanced surface enhanced Raman scattering (SERS) nanotags. The proposed method achieves high detection sensitivity and a wider linear dynamic detection range, making it suitable for rapid disease diagnosis and analysis of other substances.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Chemistry, Applied
Wen-jia Yin, Jing-xian Zhang, Hong Wang, Yu Wang, Xi Zeng, Zhen-lin Xu, Jin-yi Yang, Zhi-li Xiao, Bruce D. Hammock, Peng Wen
Summary: A sensitive and reliable electrochemical immunosensor based on cross-linked polyvinyl alcohol/citric acid nanofiber membrane (PVA/CA NFM) and horseradish peroxidase labeled anti-parathion nanobody was constructed for monitoring the residual of parathion pesticide in food. The immunosensor exhibited high specificity and sensitivity to parathion and showed good stability.
Article
Chemistry, Analytical
Cheng Peng, Jun-Fa Liang, Lin-Feng Jiang, Huang-Yi Deng, Ke Liang, Bin-Bin Zhang, Jia-Jian Lin, Yun-Ting Yi, Pei-Yi Chen, Yue-Ming Chen, Wei-Yi Cai, Gui-Yun Chen, Qiu-Xiong Ye, Zhenzhong Chen, Xuncai Chen
Summary: A sensitive and quantitative on-site detecting solution, monoclonal antibody-based carboxylated fluorescent microsphere immunochromatographic test strip assay (FM-ICTS), is proposed for the detection of florfenicol (FF) in eggs. The method demonstrates sensitivity in detecting low concentrations of FF, quantitatively measures its concentration, and exhibits high repeatability and measurement accuracy. Additionally, the test strip demonstrates high stability and can be stored for an extended period without loss of detection ability.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Chemistry, Applied
M. Graca Dias, Elsa Vasco, Francisco Ravasco, Lufsa Oliveira
Summary: This study estimated the vitamin D intake of "adults" and "elderly" populations in Portugal using the TDS methodology. The results showed that the majority of people had inadequate vitamin D intake, well below the Dietary Reference Values.
Article
Chemistry, Applied
Yanan Wang, Jiachen Shi, Yong-Jiang Xu, Chin-Ping Tan, Yuanfa Liu
Summary: This study investigates the variations in lipid digestion profiles among individuals of different ages using in vitro digestion models. The findings suggest that adults have a more comprehensive lipid digestion compared to infants, and infants tend to release shorter chain length and more saturated free fatty acids during digestion. Additionally, the particle sizes in the stomach of the elderly were consistently larger. This study enhances our understanding of how lipids with different degrees of unsaturation undergo digestion in diverse age groups.
Article
Chemistry, Applied
Hyunjong Yu, Huisu Kim, Pahn-Shick Chang
Summary: Chitosan-pectin emulsion-filled hydrogel (EFH) was developed to enhance the bioaccessibility of lipophilic bioactive compounds through intestinal delivery. The EFH, prepared without crosslinking agents, demonstrated improved mechanical strength and compactness with higher pectin concentration. It retained the emulsion at pH 2.0 and released it at pH 7.4, resulting in enhanced release of free fatty acids and improved bioaccessibility of curcumin.
Article
Chemistry, Applied
Tongze Zhang, Siqi Hong, Jia-Rong Zhang, Pin-He Liu, Siyi Li, Zixian Wen, Jianwei Xiao, Guirong Zhang, Olivier Habimana, Nagendra P. Shah, Zhongquan Sui, Harold Corke
Summary: Lactic acid fermentation significantly affects the morphology and physicochemical properties of proso millet starch, including the formation of surface indentations and small pores, decrease in gelatinization temperatures, and changes in hardness and adhesiveness.
Article
Chemistry, Applied
Liqin Kong, Feng Hong, Peng Luan, Yiping Chen, Yaoze Feng, Ming Zhu
Summary: This study presents a novel impedance biosensor using composite nanomaterials and T1R1 as a signal probe, which can competitively and ultra-sensitively detect umami intensity. The biosensor exhibits exceptional analytical performance and is suitable for food flavor evaluation.
Article
Chemistry, Applied
Kunying Lv, Yixin Yang, Qilong Li, Ran Chen, Liang Deng, Yiwei Zhang, Ning Jiang
Summary: Horse's milk, with its high nutritional value and low allergenic proteins, could be a substitute for cow's milk in infant consumption. A proteomic method was used to identify and compare milk fat globule membrane (MFGM) and whey proteins from different horse breeds. The study found differences in protein composition and functionality, which could support the development of formulas more suitable for human infants.
Article
Chemistry, Applied
Enrique Jacobo Diaz-Montana, Helene Brignot, Ramon Aparicio-Ruiz, Thierry Thomas- Danguin, Maria Teresa Morales
Summary: Sensory perception of virgin olive oil is influenced by phenols and volatiles, which are affected by the composition of the oil and biological factors. This study investigated the effect of saliva and phenols on the release of volatiles, and found that the presence of phenols decreased the release of saturated volatiles.
Article
Chemistry, Applied
Wei Zhou, Rui Zhang, Zhen Cai, Fangfang Wu, Yong Hu, Chao Huang, Kun Hu, Yun Chen
Summary: Environmentally friendly and outstanding pH-responsive cationic starch nanoparticles (CSNP) were prepared from pH-sensitive starch. CSNP exhibited nanosize and regular sphere, highly free-flowing molecular chains, and demonstrated excellent pH responsiveness through multiple emulsion/demulsification transitions.
Article
Chemistry, Applied
Andrea Koo, Vinayak Ghate, Weibiao Zhou
Summary: This study suggests that direct seeding may negatively affect the nutritional quality of crops, causing a decrease in ascorbic acid, vitamin K, and total glucosinolate content.
Article
Chemistry, Applied
Tonghao Du, Yazhou Xu, Xiaoyan Xu, Shijin Xiong, Linli Zhang, Biao Dong, Jinqing Huang, Tao Huang, Muyan Xiao, Tao Xiong, Mingyong Xie
Summary: This study successfully improved the ACE inhibitory activity of black sesame seeds by fermenting them with Lactobacillus Plantarum NCU116 and hydrolyzing them using acid protease. The RF-PSO model was used to predict the ACE inhibitory activity during the hydrolysis process. Eight peptides with ACE inhibitory activity were identified from fermented black sesame seed hydrolysates after separation and screening.
Article
Chemistry, Applied
Yao Liu, Songyi Lin, Kexin Liu, Shan Wang, Qiaozhen Liu, Na Sun
Summary: This study analyzed the structural changes of shrimp proteins during digestion, predicted the immunodominant epitopes, and validated their allergenicity. The results showed that shrimp proteins were degraded into peptides during digestion, but still carried IgE epitopes that trigger allergic reactions.
Article
Chemistry, Applied
Tiantian Fu, Hongwei Cao, Yu Zhang, Xiao Guan
Summary: This study investigates the impact of milling on the active components in rice, with a focus on the stability and bioaccessibility of phenols, VB1, and alpha-GABA during cooking and digestion. The findings show that milling exacerbates the instability of gamma-GABA during cooking and VB1 during digestion, and it affects the bioaccessibility of these active compounds.
Article
Chemistry, Applied
Zhihao Yang, Yanru Hou, Min Zhang, Puxin Hou, Chang Liu, Lu Dou, Xiaoyu Chen, Lihua Zhao, Lin Su, Ye Jin
Summary: This study investigated the molecular mechanism of feeding regimes on lamb flavor by using TMT labeling combined with MS. The results showed that pasture-fed groups had higher levels of amino acids and volatile flavor substances compared to concentrate-fed groups. Additionally, several differentially abundant proteins associated with lamb flavor were identified.
Article
Chemistry, Applied
Zixuan Xie, De Zhang, Junyu Zhu, Qianqian Luo, Jun Liu, Jingtao Zhou, Xiaoyong Wang, Yuqiong Chen, Zhi Yu, Dejiang Ni
Summary: This study investigated the acidification of aroma-enhanced black tea during storage. Analysis of non-volatile substances and organic acids using UPLC-Q-TOF/MS and HPLC revealed a decrease in soluble sugars and amino acids, while an increase in organic acids such as oxalic acid, malic acid, and quinic acid. In vitro experiments further demonstrated that the acidification is a result of the decomposition of sugars and amino acids by heating, as well as the oxidation of aromatic aldehydes. Additionally, the study showed that the taste composition of tea infusion is altered, with reduced amino acids, catechins, soluble sugars, and flavonoids. This research provides a theoretical basis for improving the quality of black tea.
Article
Chemistry, Applied
Lin Wang, Falai Ma, Zihan Li, Yan Zhang
Summary: This study developed time-temperature integrators based on amyloglucosidase@Cu3(PO4)2 nanoflowers for monitoring the freshness of chilled pork. The results showed that the integrators were highly reliable and accurate in predicting the quality of chilled pork.