期刊
FITOTERAPIA
卷 152, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.fitote.2021.104909
关键词
SARS-CoV-2 3CL(pro); Ginkgo biloba leaves; Bioflavones; Ginkgolic acids; Inhibitory mechanism
资金
- National Key Research and Development Program of China [2020YFC0845400, 2017YFC1700200, 2017YFC1702000]
- Shanghai Science and Technology Innovation Action Plans - Shanghai Science and Technology Committee [20S21901500, 20S21900900]
- Threeyear Action Plan of Shanghai TCM Development [ZY (2018-2020)-CCCX-5001]
- Shuguang Program [18SG40]
- Shanghai Education Development Foundation
- Shanghai Municipal Education Commission
- Program of Shanghai Academic/Technology Research Leader [18XD1403600]
- Program for Key Research and Development of Xinjiang Province [2017B03013]
This study aimed to discover naturally occurring SARS-CoV-2 3CL(pro) inhibitors from herbal constituents, with Ginkgo biloba leaves extract (GBLE) identified as the most potent inhibitor. The major constituents in GBLE exhibited strong SARS-CoV-2 3CL(pro) inhibition activities, offering promising compounds for developing novel anti-COVID-19 medications.
3-Chymotrypsin-like protease (3CL(pro)) is a virally encoded main proteinase that is pivotal for the viral replication across a broad spectrum of coronaviruses. This study aims to discover the naturally occurring SARS-CoV-2 3CL(pro) inhibitors from herbal constituents, as well as to investigate the inhibitory mechanism of the newly identified efficacious SARS-CoV-2 3CL(pro) inhibitors. Following screening of the inhibitory potentials of eighty herbal products against SARS-CoV-2 3CL(pro), Ginkgo biloba leaves extract (GBLE) was found with the most potent SARSCoV-2 3CL(pro) inhibition activity (IC50 = 6.68 mu g/mL). Inhibition assays demonstrated that the ginkgolic acids (GAs) and the bioflavones isolated from GBLE displayed relatively strong SARS-CoV-2 3CL(pro) inhibition activities (IC50 < 10 mu M). Among all tested constituents, GA C15:0, GA C17:1 and sciadopitysin displayed potent 3CL(pro) inhibition activities, with IC50 values of less than 2 mu M. Further inhibition kinetic studies and docking simulations clearly demonstrated that two GAs and sciadopitysin strongly inhibit SARS-CoV-2 3CL(pro) via a reversible and mixed inhibition manner. Collectively, this study found that both GBLE and the major constituents in this herbal product exhibit strong SARS-CoV-2 3CL(pro) inhibition activities, which offer several promising leading compounds for developing novel anti-COVID-19 medications via targeting on 3CL(pro).
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