Review
Obstetrics & Gynecology
Irene Dimitriadis, Nikica Zaninovic, Alejandro Chavez Badiola, Charles L. Bormann
Summary: Traditional morphological assessment of embryos can lead to variability in IVF success rates. To improve success rates, multiple embryos are often transferred, resulting in high-risk multiple pregnancies. Artificial intelligence is increasingly being used in the embryology laboratory to enhance IVF outcomes.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Editorial Material
Obstetrics & Gynecology
Carlos Simon
Summary: The goal of this series was to gather thought leaders to envision the future of laboratories. This consensus piece aims to develop themes and concepts significant for laboratories in the coming years.
FERTILITY AND STERILITY
(2021)
Editorial Material
Obstetrics & Gynecology
Victoria S. Jiang, Charles L. Bormann
Summary: The integration of AI and deep learning algorithms in medical care, particularly in the field of assisted reproductive technologies and IVF, has been a focus of development over the last decade. AI algorithms provide reliable, objective, and timely assessments for various processes in the IVF laboratory, improving clinical outcomes and laboratory efficiency. This review discusses the expanding applications of AI algorithms in IVF embryology, covering multiple aspects of the IVF process.
FERTILITY AND STERILITY
(2023)
Article
Genetics & Heredity
Nicole O. McPherson, Andrew D. Vincent, Leanne Pacella-Ince, Kelton Tremellen
Summary: The study found that for women of advanced maternal age with non-male factor infertility, the use of standard IVF insemination increased the chance of live birth compared to ICSI insemination. Other outcomes such as fertilisation rate, embryo utilisation, blastocyst transfer, and miscarriage rate were not affected by the choice of insemination method.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)
Editorial Material
Obstetrics & Gynecology
Carlos Simon, Alison Campbell, David K. Gardner, Marcos Meseguer, Kathleen A. Miller, Markus Montag, Gianpiero D. Palermo, Stephanie Cheung, Derek Keating, Philip Xie, Zev Rosenwaks, Laura Rienzi, Federica Innocenti, Danilo Cimadomo, Filippo Maria Ubaldi, Denny Sakkas, Michael J. Tucker, Liesl Nel-Themaat
Summary: The in vitro fertilization and andrology laboratories are at the forefront of assisted reproductive technologies, constantly evolving in techniques, technology, and testing to achieve the best embryo for transfer. The Views and Interviews series aims to gather thought leaders in the field to envision the future of these laboratories in the next 10 years.
FERTILITY AND STERILITY
(2021)
Article
Obstetrics & Gynecology
Kaitlyn Wald, Eduardo Hariton, Jerrine R. Morris, Ethan A. Chi, Eleni G. Jaswa, Marcelle I. Cedars, Charles E. McCulloch, Mitchell Rosen
Summary: The study showed that repeating the same ovarian stimulation protocol led to a slight but significant improvement in laboratory outcomes, suggesting careful consideration should be given before switching protocols for the purpose of improving laboratory outcomes.
FERTILITY AND STERILITY
(2021)
Article
Obstetrics & Gynecology
Darren K. Griffin
Summary: The controversy surrounding preimplantation genetic testing for aneuploidies (PGT-A) revolves around the interpretation of existing evidence, approach to euploid/aneuploid mosaicism, stance of regulatory authorities, motivation of opinion holders, and the need for finding common ground. Providing non-directive counseling to patients, summarizing the entire evidence base, and allowing them to make their own decisions seems to be the way forward.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Microbiology
Karissa Culbreath, Heather Piwonka, John Korver, Mir Noorbakhsh
Summary: This study is the first large, multicenter study in North America to report on the benefits derived from laboratory automation measured in full-time equivalents (FTE), FTE reallocation, productivity, cost per specimen, and cost avoidance. The results show that regardless of the size of the facility, improved efficiencies can be realized after implementation of full laboratory automation.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Chemistry, Physical
Lucas Eddy, Duy Xuan Luong, Jacob L. Beckham, Kevin M. Wyss, Tyler J. Cooksey, Phelecia Scotland, Chi Hun Choi, Weiyin Chen, Paul A. Advincula, Zhiyong Zhang, Vladimir Mancevski, Carter Kittrell, Yimo Han, James M. Tour
Summary: The flash Joule heating method converts carbon feedstocks into graphene using an electrical pulse. This study demonstrates the synthesis of turbostratic flash graphene from coal-based metallurgical coke using an automated FJH system. The method is energy and cost efficient, as shown by life cycle and technoeconomic assessments. Automated kilogram-scale graphene production is achieved, making it a promising technique for upcycling low value resources.
Article
Health Care Sciences & Services
Marija Dordevic, Maja Zivotic, Sanja Radojevic Skodric, Jelena Nesovic Ostojic, Jasmina Markovic Lipkovski, Jelena Filipovic, Sanja Cirovic, Sanjin Kovacevic, Dusko Dunderovic
Summary: The COVID-19 pandemic has led to significant changes in the healthcare system, with automation of the IHC procedure reducing time and costs, contributing significantly to the sustainability of the healthcare system during the pandemic by overcoming insufficient human resources.
Article
Obstetrics & Gynecology
Lorena Bori, Marcos Meseguer
Summary: IVF techniques are increasingly being automated in the laboratory, reducing the manual tasks of embryologists. This shift towards automation will lead to the emergence of new functions related to data capture and communication with other professionals and patients.
REPRODUCTIVE BIOMEDICINE ONLINE
(2021)
Article
Immunology
Weili Zhang, Siying Wu, Jin Deng, Quanfeng Liao, Ya Liu, Li Xiong, Ling Shu, Yu Yuan, Yuling Xiao, Ying Ma, Mei Kang, Dongdong Li, Yi Xie
Summary: The research investigated the changes in TAT before and after the adoption of TLA in the laboratory, revealing a significant reduction in TAT for CSF samples and improvements in TAT for other types of samples. Additionally, there was a significant decrease in the incidence of broth growth for pre-TLA compared to post-TLA.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Reproductive Biology
Brandon A. Wyse, Noga Fuchs Weizman, Janice Montbriand, Rima Kharonsky, Ran Antes, Rina Abramov, Svetlana Madjunkova, Clifford L. Librach
Summary: Different IVF-ICSI workflows have varying effects on patients depending on their primary infertility diagnosis, highlighting the importance of tailoring the workflow to individual patients. This study emphasizes the impact of IVF laboratory procedures on embryo ploidy and the need to track these outcomes in addition to traditional IVF metrics.
JOURNAL OF OVARIAN RESEARCH
(2022)
Article
Agronomy
Alexey Dorokhov, Alexander Aksenov, Alexey Sibirev, Nikolay Sazonov, Maxim Mosyakov, Maria Godyaeva
Summary: This study emphasizes the improvement in quality of sorting root tubers and onions by size through an automatic control system, eliminating manual sorting and impurities. The results show that with a forward speed of 1.2 m/s, the sorting accuracy exceeds 91%, with only 2.2% damage rate.
Article
Obstetrics & Gynecology
David K. Gardner
Summary: By introducing more technologies in the ART laboratory, outcomes such as live birth rate, time to pregnancy, and automation can be improved. New technologies have the potential to assist more patients in becoming parents of healthy children in a shorter period of time.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Anatomy & Morphology
Jennie N. Jeyapalan, Victoria. James, David S. Gardner, Jennifer H. Lothion-Roy, Nigel P. Mongan, Catrin Sian Rutland
Summary: Research is an essential part of veterinary medicine degrees, and remote learning during the COVID-19 pandemic has affected the performance and experience of students. This study analyzed the outcomes and feedback of students who completed independent research projects, showing that the pandemic cohorts achieved higher grades with increased access to resources while facing challenges in workload management and conceptual exploration.
ANATOMIA HISTOLOGIA EMBRYOLOGIA
(2023)
Letter
Obstetrics & Gynecology
Carlos Simon, Maria Ruiz-Alonso, Carlos Gomez, Diana Valbuena
FERTILITY AND STERILITY
(2023)
Article
Obstetrics & Gynecology
Kenji Ezoe, Tetsuya Miki, Hikari Akaike, Kiyoe Shimazaki, Tsubasa Takahashi, Yuko Tanimura, Ayumi Amagai, Ayano Sawado, Mai Mogi, Shigeru Kaneko, Satoshi Ueno, Giovanni Coticchio, Danilo Cimadomo, Andrea Borini, Laura Rienzi, Keiichi Kato
Summary: Maternal ageing significantly affects pronuclear size and intra-and extra-nuclear dynamics during fertilization, dysregulates cell polarity during compaction, and reduces blastocoel expansion.
HUMAN REPRODUCTION
(2023)
Article
Genetics & Heredity
Alberto Vaiarelli, Danilo Cimadomo, Carlotta Scarafia, Federica Innocenti, Maria Giulia Amendola, Gemma Fabozzi, Livio Casarini, Alessandro Conforti, Carlo Alviggi, Gianluca Gennarelli, Chiara Benedetto, Maurizio Guido, Andrea Borini, Laura Rienzi, Filippo Maria Ubaldi
Summary: This study aimed to explore the impact of different gonadotrophin selection for ovarian stimulation on oocyte competence. The results showed that the choice of recombinant FSH or human menopausal gonadotrophin for ovarian stimulation was associated with the euploid blastocyst rate. However, there was no association between the type of gonadotrophin used and live birth rate or blastocyst quality.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2023)
Letter
Obstetrics & Gynecology
Filippo Maria Ubaldi, Alberto Vaiarelli, Danilo Cimadomo, Maria Cerrillo, Laura Rienzi, Juan A. Garcia-Velasco
HUMAN REPRODUCTION
(2023)
Review
Obstetrics & Gynecology
Giovanni Coticchio, Danilo Cimadomo, Greta Chiara Cermisoni, Laura Rienzi, Enrico Papaleo, Filippo Maria Ubaldi, Andrea Borini, Lucia De Santis
Summary: Human embryos are often affected by maternally inherited aneuploidies, leading to developmental failure at early stages. Recent evidence shows that aberrant patterns at the cellular or molecular level can impact multiple stages of embryo development. Fertilization is a delicate phase where centrosomes are assembled and nuclei undergo crucial changes. Errors in this process can result in cellular or genomic integrity loss and fatal consequences for embryonic development.
HUMAN REPRODUCTION
(2023)
Article
Obstetrics & Gynecology
S. Caroselli, M. Figliuzzi, L. Picchetta, F. Cogo, P. Zambon, I Pergher, L. Girardi, C. Patassini, M. Poli, D. Bakalova, D. Cimadomo, N. Findikli, O. Coban, M. Serdarogullari, F. Favero, S. Bortolato, A. Anastasi, F. Capodanno, A. Gallinelli, F. Brancati, L. Rienzi, F. M. Ubaldi, J. Jimenez-Almazan, D. Blesa-Jarque, J. Miravet-Valenciano, C. Rubio, C. Simon, A. Capalbo
Summary: This study developed an integrated genetic testing platform that accurately detects chromosomal abnormalities, including ploidy level and microdeletions, expanding the clinical utility of preimplantation genetic testing.
HUMAN REPRODUCTION
(2023)
Review
Obstetrics & Gynecology
Danilo Cimadomo, Laura Rienzi, Alessandro Conforti, Eric Forman, Stefano Canosa, Federica Innocenti, Maurizio Poli, Jenna Hynes, Laura Gemmell, Alberto Vaiarelli, Carlo Alviggi, Filippo Maria Ubaldi, Antonio Capalbo
Summary: The normal chromosomal constitution determined by PGT-A is the strongest predictor for embryo implantation, but its positive predictive value is limited. The causes of reproductive failure in euploid blastocysts, known as "the black box of implantation," are not well understood.
HUMAN REPRODUCTION UPDATE
(2023)
Article
Genetics & Heredity
Alberto Vaiarelli, Carlotta Zaca, Valentina Spadoni, Danilo Cimadomo, Alessandro Conforti, Carlo Alviggi, Roberto Palermo, Carlo Bulletti, Lucia De Santis, Valerio Pisaturo, Vincenzo Vigiliano, Giulia Scaravelli, Filippo Maria Ubaldi, Andrea Borini
Summary: This study aims to define reliable key performance indicators (KPIs) for monitoring clinical IVF performance, and proposes a set of KPIs, performance indicators (PIs) and recommendation indicators (RIs) that encompass various essential aspects of a modern IVF clinic, including quality control and constant monitoring of clinical and embryological features. These indicators can be used to assess the quality of each center and improve the efficacy and efficiency of IVF.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2023)
Article
Multidisciplinary Sciences
Stephanie Cheung, Rony Elias, Philip Xie, Zev Rosenwaks, Gianpiero D. D. Palermo
Summary: The desire for specific sex of offspring has been a long-standing issue, especially with the development of assisted reproduction. This study proposed a novel technique for sperm sex selection that appears to be consistently safe and effective, with positive clinical outcomes.
Article
Cell Biology
Jose Ramon Hernandez Mora, Claudia Buhigas, Stephen Clark, Raquel Del Gallego Bonilla, Dagne Daskeviciute, Ana Monteagudo-Sanchez, Maria Eugenia Poo-Llanillo, Jose Vicente Medrano, Carlos Simon, Marcos Meseguer, Gavin Kelsey, David Monk
Summary: During pre-implantation stages, stored material in mammalian development promotes epigenetic profile erasure and genome activation. Through single-cell methylome and transcriptome sequencing, we analyzed the expression and methylation in oocytes and human embryos, identifying key events in epigenetic reprogramming and genome activation. Failure to achieve these milestones negatively impacts the developmental potential of embryos and may affect the success of assisted reproductive cycles.
Article
Obstetrics & Gynecology
Jessica A. Marinaro, Aaron Brant, Caroline Kang, Nahid Punjani, Philip Xie, Nikica Zaninovic, Gianpiero D. Palermo, Zev Rosenwaks, Peter N. Schlegel
Summary: Providing fresh ejaculated semen samples allows approximately 10% of men with azoospermia to avoid surgery and achieve satisfactory intracytoplasmic sperm injection (ICSI) outcomes. Additionally, providing multiple ejaculated samples over a short period of time does not have a negative impact on sperm concentration and may enhance sperm motility in men with cryptozoospermia.
FERTILITY AND STERILITY
(2023)
Article
Obstetrics & Gynecology
Philip Xie, Olena M. Kocur, Stephanie Cheung, Lily Ng, David F. Albertini, Zev Rosenwaks, Gianpiero D. Palermo
Summary: The sperm centrosome, an important organelle in fertilization and embryonic development, plays a crucial role in mitotic spindle formation, chromosome segregation, and syngamy. Abnormalities of the sperm centrosome can lead to abnormal embryonic development, pregnancy loss, and chromosomal instability. Understanding the molecular composition, regulation, and function of the sperm centrosome is vital for elucidating the mechanisms of successful fertilization and embryonic development, as well as addressing infertility and developmental disorders associated with centrosomal defects.
FERTILITY AND STERILITY
(2023)
Editorial Material
Obstetrics & Gynecology
Munevver Serdarogullari, George Liperis, Kashish Sharma, Omar F. Ammar, Julia Uraji, Danilo Cimadomo, Alessandra Alteri, Mina Popovic, Juan J. Fraire-Zamora
HUMAN REPRODUCTION
(2023)
Review
Physiology
Inmaculada Moreno, Antonio Capalbo, Aymara Mas, Tamara Garrido-Gomez, Beatriz Roson, Maurizio Poli, Eva Dimitriadis, Xavier Santamaria, Felipe Vilella, Carlos Simon
Summary: Pregnancy is established during the periconceptional period from blastocyst attachment to placenta formation, significantly impacting the health of both the child and the mother. Advances in understanding the periconceptional space, including the preimplantation human embryo and maternal endometrium, offer opportunities for preventative interventions. This review discusses the role of maternal decidua, the maternal-embryonic interface, the endometrial microbiome, and the myometrium in pregnancy health during the periconceptional period.
PHYSIOLOGICAL REVIEWS
(2023)
