期刊
FEBS LETTERS
卷 590, 期 14, 页码 2106-2118出版社
WILEY
DOI: 10.1002/1873-3468.12250
关键词
Bacteroides ovatus; galactomannan modification; GH36 alpha-galactosidase; polysaccharide utilization locus
资金
- Swedish Foundation [RBP 14-0046]
- Swedish Innovation Agency [2013-03024]
- Swedish Research Agency FORMAS [213-2014-1254]
- Vinnova [2013-03024] Funding Source: Vinnova
The Bacova_02091 gene in the beta-mannan utilization locus of Bacteroides ovatus encodes a family GH36 alpha-galactosidase (BoGal36A), transcriptionally upregulated during growth on galactomannan. Characterization of recombinant BoGal36A reveals unique properties compared to other GH36 alpha-galactosidases, which preferentially hydrolyse terminal alpha-galactose in raffinose family oligosaccharides. BoGal36A prefers hydrolysing internal galactose substitutions from intact and depolymerized galactomannan. BoGal36A efficiently releases (> 90%) galactose from guar and locust bean galactomannans, resulting in precipitation of the polysaccharides. As compared to other GH36 structures, the BoGal36A 3D model displays a loop deletion, resulting in a wider active site cleft which likely can accommodate a galactose-substituted polymannose backbone.
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