Recent advances in intravital microscopy for preclinical research

Genetically encodable materials for non-invasive biological imaging

(2021) Arash Farhadi et al.   NATURE MATERIALS

High-throughput, multi-parametric, and correlative fluorescence lifetime imaging

(2020) Chetan Poudel et al.   Methods and Applications in Fluorescence

Coregistered Spectral Optical Coherence Tomography and Two‐Photon Microscopy for Multimodal Near‐Instantaneous Deep‐Tissue Imaging

(2020) Asylkhan Rakhymzhan et al.   CYTOMETRY PART A

Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications

(2020) Rupsa Datta et al.   JOURNAL OF BIOMEDICAL OPTICS

Adaptive optics two-photon microscopy enables near-diffraction-limited and functional retinal imaging in vivo

(2020) Zhongya Qin et al.   Light-Science & Applications

Correlated Multimodal Imaging in Life Sciences: Expanding the Biomedical Horizon

(2020) Andreas Walter et al.   Frontiers in Physics

Three-photon neuronal imaging in deep mouse brain

(2020) Tianyu Wang et al.   Optica

Inhibition of Oxidative Phosphorylation Reverses Bone Marrow Hypoxia Visualized in Imageable Syngeneic B-ALL Mouse Model

(2020) Mateusz Rytelewski et al.   Frontiers in Oncology

A versatile system to record cell-cell interactions

(2020) Rui Tang et al.   eLife

Adaptive optics two-photon endomicroscopy enables deep-brain imaging at synaptic resolution over large volumes

(2020) Zhongya Qin et al.   Science Advances

Functional imaging of visual cortical layers and subplate in awake mice with optimized three-photon microscopy

(2019) Murat Yildirim et al.   Nature Communications

Merger of dynamic two-photon and phosphorescence lifetime microscopy reveals dependence of lymphocyte motility on oxygen in solid and hematological tumors

(2019) Mateusz Rytelewski et al.   Journal for ImmunoTherapy of Cancer

Volumetric Ca2+ Imaging in the Mouse Brain Using Hybrid Multiplexed Sculpted Light Microscopy

(2019) Siegfried Weisenburger et al.   CELL

Applications, promises, and pitfalls of deep learning for fluorescence image reconstruction

(2019) Chinmay Belthangady et al.   NATURE METHODS

Live cell imaging of signaling and metabolic activities

(2019) Maria R. Depaoli et al.   PHARMACOLOGY & THERAPEUTICS

Artificial intelligence-assisted light control and computational imaging through scattering media

(2019) Shengfu Cheng et al.   Journal of Innovative Optical Health Sciences

Intravital microscopy in mammalian multicellular organisms

(2019) Seham Ebrahim et al.   CURRENT OPINION IN CELL BIOLOGY

Fast single-cell biochemistry: theory, open source microscopy and applications

(2019) Andrew L Trinh et al.   Methods and Applications in Fluorescence

Systematic Enzyme Mapping of Cellular Metabolism by Phasor-Analyzed Label-Free NAD(P)H Fluorescence Lifetime Imaging

(2019) Leben et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Deep Learning Reveals Cancer Metastasis and Therapeutic Antibody Targeting in the Entire Body

(2019) Chenchen Pan et al.   CELL

In vivo flow cytometry combined with intravital microscopy to monitor kinetics of transplanted bone marrow mononuclear cells in peripheral blood and bone marrow

(2019) Fen Wang et al.   MOLECULAR BIOLOGY REPORTS

Multimodal image registration and connectivity analysis for integration of connectomic data from microscopy to MRI

(2019) Maged Goubran et al.   Nature Communications

In Silico Labeling: Predicting Fluorescent Labels in Unlabeled Images

(2018) Eric M. Christiansen et al.   CELL

A Suite of Transgenic Driver and Reporter Mouse Lines with Enhanced Brain-Cell-Type Targeting and Functionality

(2018) Tanya L. Daigle et al.   CELL

Imaging of oxygen and hypoxia in cell and tissue samples

(2018) Dmitri B. Papkovsky et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Proteins, air and water: reporter genes for ultrasound and magnetic resonance imaging

(2018) George J Lu et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Deep learning massively accelerates super-resolution localization microscopy

(2018) Wei Ouyang et al.   NATURE BIOTECHNOLOGY

Three-photon fluorescence microscopy with an axially elongated Bessel focus

(2018) Cristina Rodríguez et al.   OPTICS LETTERS

Direct cell–cell contact between mature osteoblasts and osteoclasts dynamically controls their functions in vivo

(2018) Masayuki Furuya et al.   Nature Communications

Rapid volumetric imaging with Bessel-Beam three-photon microscopy

(2018) Bingying Chen et al.   Biomedical Optics Express

Intravital microscopy in the study of the tumor microenvironment: from bench to human application

(2018) Emmanuel M. Gabriel et al.   Oncotarget

Intravital Imaging to Monitor Therapeutic Response in Moving Hypoxic Regions Resistant to PI3K Pathway Targeting in Pancreatic Cancer

(2018) James R.W. Conway et al.   Cell Reports

Dual-color deep-tissue three-photon microscopy with a multiband infrared laser

(2018) Khmaies Guesmi et al.   Light-Science & Applications

Mapping metabolic changes by noninvasive, multiparametric, high-resolution imaging using endogenous contrast

(2018) Zhiyi Liu et al.   Science Advances

Emerging Technologies to Image Tissue Metabolism

(2018) Vasilis Ntziachristos et al.   Cell Metabolism

Monitoring T cell–dendritic cell interactions in vivo by intercellular enzymatic labelling

(2018) Giulia Pasqual et al.   NATURE

Label-free prediction of three-dimensional fluorescence images from transmitted-light microscopy

(2018) Chawin Ounkomol et al.   NATURE METHODS

Three-photon imaging of mouse brain structure and function through the intact skull

(2018) Tianyu Wang et al.   NATURE METHODS

Content-aware image restoration: pushing the limits of fluorescence microscopy

(2018) Martin Weigert et al.   NATURE METHODS

U-Net: deep learning for cell counting, detection, and morphometry

(2018) Thorsten Falk et al.   NATURE METHODS

Panoptic imaging of transparent mice reveals whole-body neuronal projections and skull–meninges connections

(2018) Ruiyao Cai et al.   NATURE NEUROSCIENCE

Intravital microscopy in historic and contemporary immunology

(2017) Judith Secklehner et al.   IMMUNOLOGY AND CELL BIOLOGY

Adaptive optical fluorescence microscopy

(2017) Na Ji   NATURE METHODS

The Growing and Glowing Toolbox of Fluorescent and Photoactive Proteins

(2017) Erik A. Rodriguez et al.   TRENDS IN BIOCHEMICAL SCIENCES

Third harmonic generation microscopy of cells and tissue organization

(2016) Bettina Weigelin et al.   JOURNAL OF CELL SCIENCE

Real-time intravital imaging of pH variation associated with osteoclast activity

(2016) Hiroki Maeda et al.   Nature Chemical Biology

Hypoxic stress: obstacles and opportunities for innovative immunotherapy of cancer

(2016) S Chouaib et al.   ONCOGENE

Live Imaging of Immune Responses in Experimental Models of Multiple Sclerosis

(2016) Barbara Rossi et al.   Frontiers in Immunology

Molecular Probes for Fluorescence Lifetime Imaging

(2015) Pinaki Sarder et al.   BIOCONJUGATE CHEMISTRY

Thalamus provides layer 4 of primary visual cortex with orientation- and direction-tuned inputs

(2015) Wenzhi Sun et al.   NATURE NEUROSCIENCE

Direct measurement of local oxygen concentration in the bone marrow of live animals

(2014) Joel A. Spencer et al.   NATURE

In vivo three-photon microscopy of subcortical structures within an intact mouse brain

(2013) Nicholas G. Horton et al.   Nature Photonics

Optical properties of biological tissues: a review

(2013) Steven L Jacques   PHYSICS IN MEDICINE AND BIOLOGY

Single-cell and subcellular pharmacokinetic imaging allows insight into drug action in vivo

(2013) Greg M. Thurber et al.   Nature Communications

Intravital Imaging

(2011) Mikael J. Pittet et al.   CELL

Cancer Invasion and the Microenvironment: Plasticity and Reciprocity

(2011) Peter Friedl et al.   CELL

Simultaneous two-photon imaging of oxygen and blood flow in deep cerebral vessels

(2011) Jérôme Lecoq et al.   NATURE MEDICINE

Cytokine reporter mice in immunological research: perspectives and lessons learned

(2010) Andrew L. Croxford et al.   IMMUNOLOGY

Infrared multiphoton microscopy: subcellular-resolved deep tissue imaging

(2009) Volker Andresen et al.   CURRENT OPINION IN BIOTECHNOLOGY