Bifunctional recombinant cellulase-xylanase (rBhcell-xyl) from the polyextremophilic bacterium Bacillus halodurans TSLV1 and its utility in valorization of renewable agro-residues

The thermostable bifunctional CMCase and xylanase encoding gene (rBhcell-xyl) from Bacillus halodurans TSLV1 has been expressed in Escherichia coli. The recombinant E. coli produced rBhcell-xyl (CMCase 2272 and 910 U L-1 xylanase). The rBhcell-xyl is a similar to 62-kDa monomeric protein with temperature and pH optima of 60 A degrees C and 6.0 with T-1/2 of 7.0 and 3.5 h at 80 A degrees C for CMCase and xylanase, respectively. The apparent K (m) values (CMC and Birchwood xylan) are 3.8 and 3.2 mg mL(-1). The catalytic efficiency (k (cat)/K (m) ) values of xylanase and CMCase are 657 and 171 mL mg(-1) min(-1), respectively. End-product analysis confirmed that rBhcell-xyl is a unique endo-acting enzyme with exoglucanase activity. The rBhcell-xyl is a GH5 family enzyme possessing single catalytic module and carbohydrate binding module. The action of rBhcell-xyl on corn cobs and wheat bran liberated reducing sugars, which can be fermented to bioethanol and fine biochemicals.