期刊
CURRENT OPINION IN BIOTECHNOLOGY
卷 69, 期 -, 页码 290-298出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.copbio.2021.02.010
关键词
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资金
- Center of Biomedical Research Excellence (COBRE) in Pharmaceutical Research and Innovation (CPRI) [N.I.H.P20 GM130456]
- University of Kentucky College of Pharmacy
- National Center for Advancing Translational Sciences [UL1TR000117, UL1TR001998]
- [R37 AI52218]
- [R01 GM115261]
Transferases are highlighted as effective catalysts for enzyme-mediated bioorthogonal functional group installation in various biological applications. The focus of this review is on the key technologies and applications of methyltransferases (MTs) in bioorthogonal labeling and conjugation reactions involving DNA, RNA, proteins, and natural products.
Transferases have emerged as among the best catalysts for enzyme-mediated bioorthogonal functional group installation to advance innovative in vitro, cell-based and in vivo chemical biology applications. This review introduces the key considerations for selecting enzyme catalysts and chemoselective reactions most amenable to bioorthogonal platform development and highlights relevant key technology development and applications for one ubiquitous transferase subclass - methyltransferases (MTs). Within this context, recent advances in MT-enabled bioorthogonal labeling/conjugation relevant to DNA, RNA, protein, and natural products (i.e. complex small molecule metabolites) are highlighted.
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