Article
Ecology
Uriel Arreguin Rebolledo, Roberto Rico-Martinez, Rocio Fernandez, Federico Paez-Osuna
Summary: This study evaluated the toxicity of chloroquine (CQ) alone and in combination with copper (Cu) to Proales similis and found that these combined chemicals may have potential hazards on aquatic systems.
Article
Biotechnology & Applied Microbiology
Duck-Hyun Kim, Eunjin Byeon, Min-Sub Kim, Young Hwan Lee, Jun Chul Park, Atsushi Hagiwara, Jae-Seong Lee
Summary: In this study, the whole-genome sequence of the marine rotifer Brachionus manjavacas was assembled, and 310 GPCR genes were identified and classified. The results provide clues for studying GPCR-based signaling pathways in B. manjavacas and expand our understanding of the response of rotifers to various environmental signals.
MARINE BIOTECHNOLOGY
(2022)
Article
Environmental Sciences
Uriel Arreguin-Rebolledo, Federico Paez-Osuna, Miguel Betancourt-Lozano, Roberto Rico-Martinez
Summary: This study evaluated the multi-and transgenerational effects of the commonly found pesticides glyphosate (GLY) and chlorpyrifos (CPF) in the estuarine rotifer Proales similis. The results showed that the mixture of GLY and CPF had a strong toxic effect and significantly decreased the growth rates of the rotifers. This suggests that the presence of a mixture of GLY and CPF in the environment can pose a risk to marine and estuarine invertebrates.
ENVIRONMENTAL POLLUTION
(2023)
Article
Environmental Sciences
Uriel Arreguin Rebolledo, Federico Paez-Osuna, Rocio Fernandez
Summary: This study investigates the toxicity of nine trace metals to a rotifer under different salinity conditions, finding higher toxicity at low salinity and an intensification in single metal toxicity with increasing application factor and decreasing salinity. Lead was identified as the most toxic metal, while arsenic treatment showed enhanced growth for Proales similis. Treatments T1 and T2 were found to be the most toxic and often induced a synergistic effect.
ENVIRONMENTAL POLLUTION
(2021)
Article
Environmental Sciences
Uriel Arreguin-Rebolledo, Renan Castelhano Gebara, Gladys Valencia-Castaneda, Roberto Rico-Martinez, Martin G. Frias-Espericueta, Elson Longo, Federico Paez-Osuna
Summary: Data is lacking on the effects of binary metal mixtures on marine zooplankton, especially rotifers. This study examined the toxicity of 21 equitoxic binary mixtures of As, Cd, Cu, Fe, Hg, Pb, and Zn on the euryhaline rotifer Proales similis. The results showed that the majority of these mixtures exhibited synergistic effects, highlighting the need for revising protection guidelines for marine biota.
MARINE POLLUTION BULLETIN
(2024)
Article
Biochemistry & Molecular Biology
Shuai Luo, Peng Zhang, Wei Miao, Jie Xiong
Summary: This study provides the first comprehensive genome-wide identification of GPCRs in ciliates, identifying 492 GPCRs in 24 ciliates. GPCRs in ciliates can be assigned to four families, with most belonging to family A. Gene duplication events play a role in the expansion of the GPCR superfamily in ciliates. This study improves our understanding of the evolution and function of GPCRs in ciliates.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Haoran Jiang, Daniella Galtes, Jialu Wang, Howard A. Rockman
Summary: This review explores the signaling pathways, dynamic structures, and physiological relevance of the three most important GPCR signaling effectors in the cardiovascular system: heterotrimeric G proteins, GPCR kinases (GRKs), and 8-arrestins. It summarizes their prominent roles in GPCR pharmacology before transitioning into less well-explored areas. The application of new technologies has contributed to an increasing understanding of GPCR structure and downstream effectors.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Estefania Moreno, Nil Casajuana-Martin, Michael Coyle, Baruc Campos Campos, Ewa Galaj, Claudia Llinas del Torrent, Arta Seyedian, William Rea, Ning-Sheng Cai, Alessandro Bonifazi, Benjamin Floran, Zheng-Xiong Xi, Xavier Guitart, Vicent Casado, Amy H. Newman, Christopher Bishop, Leonardo Pardo, Sergi Ferre
Summary: This study provides evidence that heteromerization of G protein-coupled receptors (GPCRs), specifically dopamine D1 and D3 receptors, can influence the pharmacological properties of selective ligands. In vivo experiments support the involvement of D1R-D3R heteromers in the development of L-DOPA-induced dyskinesia in Parkinson's disease, suggesting the potential of targeting GPCR heteromers for drug development.
PHARMACOLOGICAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Juergen Einsiedel, Maximilian F. Schmidt, Harald Huebner, Peter Gmeiner
Summary: A broadly applicable synthesis method was developed for peptides incorporating mixed disulfides between cysteine and homocysteine and cysteamine. The method was successfully applied to pharmacologically relevant GPCR ligands and showed covalent binding to neurotensin receptor 1 in a radioligand depletion study.
BIOORGANIC & MEDICINAL CHEMISTRY
(2022)
Review
Pharmacology & Pharmacy
Bui San Thai, Ling Yeong Chia, Anh T. N. Nguyen, Chengxue Qin, Rebecca H. Ritchie, Dana S. Hutchinson, Andrew Kompa, Paul J. White, Lauren T. May
Summary: Heart failure remains a significant cause of morbidity and mortality worldwide. Current treatment options have limitations, leading to many patients progressing to advanced stages. Exploration of novel therapeutics targeting G protein-coupled receptors (GPCRs) has shown promise, but efficacy and unwanted effects remain as challenges.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Review
Pharmacology & Pharmacy
Mydirah Littlepage-Saunders, Michael J. Hochstein, Doris S. Chang, Kari A. Johnson
Summary: Dopamine transmission in the striatum is regulated by various G protein-coupled receptors (GPCRs) that bind neuromodulators, including dopamine itself. These GPCRs can modulate dopamine release by acting on different components of the dopaminergic circuitry and can have distinct effects on behavior and psychoactive drug actions. This review discusses the mechanisms by which GPCRs regulate dopaminergic transmission and their relevance to the effects of psychoactive drugs on physiology and behavior.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Damian Jacenik, Pawel Hikisz, Ellen J. Beswick, Jakub Fichna
Summary: Among the various adhesion G protein-coupled receptors, ADGRF5 stands out with its unique domains in the N-terminal tail that play a critical role in cell-cell and cell-matrix interactions, as well as cell adhesion. Although the biology of ADGRF5 is still not fully understood, accumulating evidence suggests its fundamental importance in both health and disease. Recent studies have highlighted its potential diagnostic value in osteoporosis and cancers, and ongoing research indicates its relevance to other diseases as well. This article provides a comprehensive overview of the current understanding of ADGRF5 in human disease physiology and pathophysiology, emphasizing its potential as a novel therapeutic target in various areas.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2023)
Review
Biochemistry & Molecular Biology
Raudah Lazim, Donghyuk Suh, Jai Woo Lee, Thi Ngoc Lan Vu, Sanghee Yoon, Sun Choi
Summary: The presence of GPCR dimers has sparked research into their importance in disease pathogenesis and drug design, uncovering new signaling pathways and potential therapeutic targets. The increasing influence of computational methods in research is providing new avenues for understanding the functions and interactions of GPCRs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Pharmacology & Pharmacy
Alastair C. Keen, Manuela Jorg, Michelle L. L. Halls
Summary: The ubiquitin-proteasome system is a major pathway for protein degradation in cells, and methods have been developed to exploit this system for targeted protein degradation. Targeted protein degraders have been useful tools in discovery research and are being developed as therapeutics. However, most targeted protein degrader technologies have been developed for cytosolic proteins, while examples for G protein-coupled receptor (GPCR) degradation are limited. This review discusses the strategies used for applying targeted protein degradation to GPCRs and explores alternative approaches used for degrading other integral membrane proteins.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Review
Pharmacology & Pharmacy
Rina Pokhrel, Alexandra L. Morgan, Harley R. Robinson, Martin J. Stone, Simon R. Foster
Summary: G protein-coupled receptor (GPCR) activation triggers complex intracellular signalling networks, which have important implications for receptor biology and drug discovery. Phosphoproteomics has emerged as a powerful tool for investigating these networks and accelerating the discovery of new therapeutic targets.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Ecology
Deok-Seo Yoon, Hyuntae Choi, Alaa El -Din H. Sayed, Kyung-Hoon Shin, Joung Han Yim, Sanghee Kim, Min-Chul Lee, Jae-Seong Lee
Summary: Changes in temperature and starvation affect metabolism and fatty acid composition in zooplankton. However, little is known about fatty acid modulation in Antarctic zooplankton. This study investigated the changes in life history parameters and fatty acid profiles in the Antarctic copepod Tigriopus kingsejongensis under starvation at four different temperatures. The results showed that higher temperatures accelerated the development of T. kingsejongensis and increased overall offspring production. Starvation and higher temperatures led to a decrease in polyunsaturated fatty acids (PUFAs) and an increase in saturated fatty acids in T. kingsejongensis. However, specific PUFAs, such as docosahexaenoic acid and docosapentaenoic acid, increased under starvation and higher temperatures. This study provides valuable insights into fatty acid regulation in Antarctic copepods under environmental stressors.
REGIONAL STUDIES IN MARINE SCIENCE
(2023)
Article
Engineering, Environmental
Young Hwan Lee, Min -Sub Kim, Yoseop Lee, Duck-Hyun Kim, Jae-Seong Lee
Summary: Ocean acidification (OA) and nanoplastics (NPs) have a synergistic negative effect on copepod reproduction across generations, potentially posing a significant threat to the sustainability of copepod populations. The intergenerational reproductive impairments observed are associated with differential methylation patterns of specific genes, indicating the role of epigenetic modifications. These findings contribute valuable insight into the intergenerational toxicity and underlying molecular mechanisms of responses to NPs under OA conditions.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Engineering, Environmental
Zhuoan Bai, Nan Wang, Min-Sub Kim, Young Hwan Lee, Jae-Seong Lee, Da-Zhi Wang, Minghua Wang
Summary: This study investigated the effects of warmer temperature (26 degrees C) and mercury pollution (1 mu g/L) on the marine copepod Tigriopus japonicus across three generations (F0-F2). Phenotypic traits, mercury accumulation, and proteomic analysis were performed. The results showed that both warmer temperature and mercury exposure significantly impacted the phenotypic traits. Combined exposure increased mercury bioaccumulation and its toxicity in each generation. Proteomic analysis revealed that warmer temperature upregulated the determination of adult lifespan pathway but compromised fitness. Mercury pollution caused energy depletion and reduced defense performance, leading to decreased reproduction. Importantly, warmer temperature aggravated mercury toxicity by disrupting energy metabolism and impairing detoxification systems. This study provides a mechanistic understanding of T. japonicus response to coastal warming and mercury pollution.
ENVIRONMENTAL SCIENCE & TECHNOLOGY LETTERS
(2023)
Article
Zoology
M. Pyznar, P. Maszczyk, B. Kiersztyn, K. Dabrowski, M. L. Zebrowski, J. -s. Lee, E. Babkiewicz
Summary: Very few studies have examined the influence of fish on the degradation rate of microplastic particles. This study aimed to test the short-term effects of fish presence on water chemical parameters and the abundance and genetic diversity of bacteria colonizing microplastics. The presence of fish increased the concentration of certain chemicals in the water and the number of bacteria, which in turn led to an increase in the abundance and diversity of bacteria on the surface of microplastics.
EUROPEAN ZOOLOGICAL JOURNAL
(2023)
Article
Fisheries
Jun Bo, Ronghui Zheng, Yulu Jiang, Jincan Chen, Chao Fang, Christyn Bailey, Yusheng Zhang, Jae-Seong Lee, Helmut Segner
Summary: Global warming and changes in environmental conditions may impact the health of marine species. This study examined the liver transcriptome and proteome of the hybrid grouper to understand the physiological pathways affected by climate change-related stressors. The results revealed time-dependent and stressor-specific responses, with elevated temperature having a dominant effect.
Article
Marine & Freshwater Biology
Yoseop Lee, Eunjin Byeon, Duck-Hyun Kim, Piotr Maszczyk, Minghua Wang, Rudolf Shiu Sun Wu, Hee-Do Jeung, Un-Ki Hwang, Jae-Seong Lee
Summary: Global deoxygenation is an increasing environmental problem that affects aquatic systems and has substantial effects on aquatic invertebrates. Studies have shown that exposure to hypoxia leads to decreased locomotion, respiration, feeding, growth, and reproduction rates in these animals. However, further research is needed to fully understand the molecular mechanisms and alternative defense mechanisms involved in responding to hypoxia.
AQUATIC TOXICOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Paweena Sanpradit, Eunjin Byeon, Jae-Seong Lee, Saranya Peerakietkhajorn
Summary: Climate change affects aquatic ecosystems by raising water temperature, which in turn has adverse effects on organisms. The mass production of zinc oxide during industrial activities has led to environmental contamination, making the toxicological effects of zinc oxide more concerning under climate change scenarios. Understanding the toxicity of zinc oxide under the main factors driven by climate change is crucial.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2023)
Article
Environmental Sciences
Ibrahim A. Mohamed, Hamdy A. M. Soliman, Mervat Hana, Jae-Seong Lee, Alaa El -Din H. Sayed
Summary: Acute toxicity experiments were conducted to determine the LC50 of UPGR 46% SL for Oreochromis niloticus, and the 96-h LC50 value was found to be 29.16 mg L-1. Hemato-biochemical effects were also studied, and it was discovered that UPGR exposure led to a significant decrease in various blood parameters, while sub-acute exposure resulted in an increase in certain parameters. The antagonistic toxic effects of UPGR and PE-MPs might be due to the sorption of UPGR onto PE-MPs.
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2023)
Article
Engineering, Environmental
Young Hwan Lee, Min-Sub Kim, Yoseop Lee, Chuxin Wang, Seong Chan Yun, Jae-Seong Lee
Summary: This study investigated the effects of interactions between microfibers and freshwater acidification (MFA) on individual Daphnia magna-microbiota interactions. The results showed that MFA interactions can have negative consequences on reproduction, growth, and sex ratio of D. magna, and can induce changes in immunity-and reproduction-related biological processes. The study also revealed that these adverse effects are closely related to changes in microbial communities, suggesting a causal link between D. magna fitness and the microbial community.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Environmental Sciences
Jin-Sol Lee, Yunmoon Oh, Hae Eun Park, Jae-Seong Lee, Hyung Sik Kim
Summary: In this study, the combined toxic effects of simultaneous exposure to microplastics and triclosan on Daphnia magna were investigated. It was found that simultaneous exposure resulted in increased mortality and altered antioxidant enzymatic activities compared to single exposure to triclosan. The accumulation of triclosan was also observed due to the inhibition of the multixenobiotic resistance activity by microplastics. These findings suggest the synergistic toxic effects of simultaneous exposure to microplastics and triclosan.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Engineering, Environmental
In-Cheol Yeo, Kyu-Young Shim, Kyuhyeong Kim, Young-Shin Go, Jieun Kim, Dong-Hun Lee, Jae-Seong Lee, Kyung-Hoon Shin, Chang-Bum Jeong
Summary: Despite limited understanding and analysis of the biological fate of nanoplastics, a novel stable carbon isotopic approach has been proposed to detect and quantify nano- and microplastics in complex organic matrices. By quantifying the contribution of plastic particles in the stable carbon isotopes composition in different tissues, tissue-specific bioaccumulation of nano- and microplastics in a fish species has been investigated. The findings shed light on the unique mode of action of nanoplastics' bioaccumulation based on physicochemical properties and have significance in improving our understanding of the tissue-specific bioaccumulation of nanoplastics in aquatic organisms.
ENVIRONMENTAL SCIENCE & TECHNOLOGY LETTERS
(2023)
Article
Ecology
Piotr Maszczyk, Wojciech Wilczynski, Z. Maciej Gliwicz, Konrad Leniowski, Marcin Lukasz Zebrowski, Jae-Seong Lee, Ewa Babkiewicz
Summary: The temperature-size rule (TSR) states that ectotherms reared at higher temperatures experience accelerated growth during the juvenile stage and ultimately reach smaller sizes and younger ages at maturity. This phenomenon occurs when the effect of temperature on mortality outweighs its effect on food intake and metabolism. In a study with two fish species, it was found that the capture rate, a proxy for mortality, increased significantly more than expected based on the assumption that Q(10) = 2, indicating a stronger effect of temperature on mortality. This could be attributed to the increased mobility and attack rate of the fish at elevated temperatures compared to its planktonic prey.
FRONTIERS IN ECOLOGY AND EVOLUTION
(2023)
Article
Engineering, Environmental
Min-Sub Kim, Young Hwan Lee, Yoseop Lee, Eunjin Byeon, Duck-Hyun Kim, Minghua Wang, Atsushi Hagiwara, Manuel Aranda, Rudolf Shiu Sun Wu, Heum Gi Park, Jae-Seong Lee
Summary: The susceptibility of filter-feeding rotifers to high CO2 conditions and nanoplastic exposure depends on their adaptation history. Rotifers with different adaptation histories showed differences in ingestion and accumulation of nanoplastics, which may be related to microRNA-mediated epigenetic regulation.
JOURNAL OF HAZARDOUS MATERIALS
(2024)
Article
Fisheries
Mi-Song Hong, Deok-Seo Yoon, Ji-Su Kim, Heum Gi Park, Jae-Seong Lee, Min-Chul Lee
Summary: We conducted a comprehensive analysis of the effects of increased salinity on Brachionus plicatilis, finding delays in maturation and potential disruptions in the reproductive process. High salinity also impedes growth and alters swimming behavior. Additionally, we discovered distinctive sequences of FABPs in three Brachionus species, contributing to our understanding of their evolutionary positioning.
Article
Biochemistry & Molecular Biology
Diana Medina-Felix, Francisco Vargas-Albores, Estefania Garibay-Valdez, Luis Rafael Martinez-Cordova, Marcel Martinez-Porchas
Summary: In this research, the effects of Nocardia infection on fish gastrointestinal microbiota were analyzed. It was found that the infection led to decreased survival rate, severe damage to the stomach microbiota, and a significant increase in Proteobacteria. A negative correlation network between Proteobacteria and other important phyla was observed. Therefore, Nocardia sp. is an emerging pathogen capable of inducing dysbiosis and causing significant mortalities.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Lele Wu, Wen Sun, Jiale Zhou, Yaolin Li, Jun Li, Zongcheng Song, Changbin Song, Shihong Xu, Xinlu Yue, Xian Li
Summary: The study finds that red light induces dichromatic skin pigmentation in turbot juveniles, with some individuals displaying black coloration and others displaying lighter skin. The upregulated gene expressions related to melanin synthesis and the involvement of the nervous system in spectral environment-driven color regulation are both crucial factors.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
(2024)