期刊
EXPERT REVIEW OF MOLECULAR DIAGNOSTICS
卷 16, 期 6, 页码 631-640出版社
TAYLOR & FRANCIS AS
DOI: 10.1586/14737159.2016.1167599
关键词
Gastroenteritis; diarrhea; enteropathogens; bacteria; viruses; protozoa; DNA-based tools; culture; antigen test; microscopic observation
类别
资金
- European Development Regional Fund 'A way to achieve Europe' ERDF
- Spanish Network for the Research in Infectious Diseases [REIPI RD12/0015]
- Departament d'Universitats, Recerca i Societat de la Informacio of the Generalitat de Catalunya [2014SGR0653]
- [FIS 14/0755]
Acute infections of the gastrointestinal tract are among the most common infectious diseases. The etiological agents of gastroenteritis may be bacteria, viruses or protozoa. Identification of the etiological agents of acute diarrhea is important for the treatment and management of diarrheal diseases. Conventional stool culture for bacteria shows a low sensitivity and requires more than 24 hours. In addition, other approaches to detect viruses and protozoa mainly involve antigen detection, but this is not available for all enteropathogens, and microscopic observation requires training and is of low sensitivity. In this review, the authors describe currently available molecular methods to detect different enteropathogens and analyze the main advantages and disadvantages of these methods for laboratory diagnosis of gastroenteritis.
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