Article
Multidisciplinary Sciences
Takahiro Sawada, Masayuki Katayama, Shogo Takatani, Yoshiyuki Ohiro
Summary: Early detection of drug resistance is important for combating drug-resistant bacteria and improving patient outcomes. Conventional phenotypic testing for microbial resistance is time-consuming, especially for fastidious bacteria. This study demonstrates a highly sensitive quantitative flow cytometry method for rapid antimicrobial susceptibility testing, providing a novel approach for detecting drug resistance in a few hours.
SCIENTIFIC REPORTS
(2021)
Article
Public, Environmental & Occupational Health
Shrijana Shrestha, Meeru Gurung, Puja Amatya, Sanjeev Bijukchhe, Anindya Sekhar Bose, Michael J. Carter, Madhav C. Gautam, Sunaina Gurung, Jason Hinds, Rama Kandasamy, Sarah Kelly, Bibek Khadka, Pratistha Maskey, Yama F. Mujadidi, Peter J. O'Reilly, Bhishma Pokhrel, Rahul Pradhan, Ganesh P. Shah, Sonu Shrestha, Brian Wahl, Katherine L. O'Brien, Maria Deloria Knoll, David R. Murdoch, Dominic F. Kelly, Stephen Thorson, Merryn Voysey, Andrew J. Pollard
Summary: This study investigated the impact of introducing PCV10 vaccine on pneumococcal carriage and disease in children in Nepal. The results showed a reduction in vaccine serotype carriage in both healthy children and children under 2 years with pneumonia. However, there was an increase in carriage of serotypes 19A and 3, emphasizing the importance of continued surveillance.
LANCET GLOBAL HEALTH
(2022)
Article
Microbiology
Helene Gingras, Flora Peillard-Fiorente, Chantal Godin, Kevin Patron, Philippe Leprohon, Marc Ouellette
Summary: Two strains of Streptococcus pneumoniae with or without erm(B) were selected for solithromycin resistance in vitro. Mutants with decreased solithromycin susceptibility were obtained and characterized by next-generation sequencing. Various mutations in ribosomal proteins, 23S rRNA, phosphate transporter subunits, helicase CshB, and erm(B)L leader peptide were identified.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Infectious Diseases
N. Littorin, E. Runow, J. Ahl, F. Resman, K. Riesbeck
Summary: The introduction of PCV significantly reduced the prevalence of S. pneumoniae and H. influenzae in children with respiratory tract infection, potentially due to a positive association between PCV serotypes and H. influenzae.
CLINICAL MICROBIOLOGY AND INFECTION
(2021)
Article
Immunology
Kelly M. Martinovich, Elke J. Seppanen, Amy S. Bleakley, Sharon L. Clark, Ross M. Andrews, Peter C. Richmond, Michael J. Binks, Ruth B. Thornton, Lea-Ann S. Kirkham
Summary: This study investigated the antibody levels of Streptococcus pneumoniae and Haemophilus influenzae in Australian First Nation mothers and infants. It was found that infants had low levels of H. influenzae antibodies, possibly due to low maternal antibody levels or inadequate placental transfer.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Health Care Sciences & Services
Xiaozhen Lai, Brian Wahl, Wenzhou Yu, Tingting Xu, Haijun Zhang, Cristina Garcia, Ying Qin, Yan Guo, Zundong Yin, Maria Deloria Knoll, Hai Fang
Summary: The study estimated childhood mortality and morbidity attributable to pneumococcus and Hib in China from 2010 to 2017. It found that while there was a decrease in deaths and cases, the burden still varied significantly by region and province.
LANCET REGIONAL HEALTH-WESTERN PACIFIC
(2022)
Article
Health Care Sciences & Services
Sari T. T. Alhoufie, Areej A. A. Alhhazmi, Waleed H. H. Mahallawi, Khalid O. O. Alfarouk, Nadir A. A. Ibrahim
Summary: Community-acquired atypical pneumonia caused by Chlamydia pneumoniae, Legionella pneumophila, and Mycoplasma pneumoniae is highly prevalent among healthy individuals, with a higher occurrence among males. The distribution pattern of these bacteria in healthy people differs from that in elderly patients in hospital settings.
Article
Immunology
Beth Temple, Monica Larissa Nation, Vo Thi Trang Dai, Jemima Beissbarth, Kathryn Bright, Eileen Margaret Dunne, Jason Hinds, Pham Thi Hoan, Jana Lai, Cattram Duong Nguyen, Belinda Daniela Ortika, Thanh Phan, Ho Nguyen Loc Thuy, Nguyen Trong Toan, Doan Y. Uyen, Catherine Satzke, Heidi Smith-Vaughan, Tran Ngoc Huu, Kim Mulholland
Summary: The study found that both PCV10 and PCV13 have similar effects in reducing pneumococcal carriage, with both vaccines reducing the carriage of vaccine serotypes. In addition, the distribution of serotypes among unvaccinated participants was also described.
Article
Medicine, General & Internal
Alexis Rybak, Corinne Levy, Francois Angoulvant, Anne Auvrignon, Piotr Gembara, Kostas Danis, Sophie Vaux, Daniel Levy-Bruhl, Sylvie van der Werf, Stephane Bechet, Stephane Bonacorsi, Zein Assad, Andrea Lazzati, Morgane Michel, Florentia Kaguelidou, Albert Faye, Robert Cohen, Emmanuelle Varon, Naim Ouldali
Summary: This study examined the impact of nonpharmaceutical interventions implemented during the COVID-19 pandemic on the incidence of invasive pneumococcal disease (IPD) in France. The results showed that the decrease in IPD incidence was associated with decreased viral infection cases, rather than changes in pneumococcal carriage rate.
Article
Infectious Diseases
Susan Meiring, Cheryl Cohen, Linda de Gouveia, Mignon du Plessis, Vanessa Quan, Jackie Kleynhans, Colin Menezes, Gary Reubenson, Halima Dawood, Maphoshane Nchabeleng, Mohamed Said, Nomonde Mvelase, Prasha Mahabeer, Rispah Chomba, Ruth Lekalakala, Trusha Nana, Vindana Chibabhai, Marianne Black, Anne von Gottberg
Summary: Providing country-specific estimates of case fatality and sequelae from bacterial meningitis (BM) is important to evaluate and monitor progress toward the World Health Organization's roadmap to defeating meningitis by 2030. The study conducted enhanced surveillance at 26 hospitals in South Africa from 2016-2020, revealing that BM in South Africa has a high case fatality, and adverse sequelae frequently occur among survivors. Those with comorbidities, including HIV, are at the highest risk.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Infectious Diseases
Young June Choe, Mi Seon Han, Youn Young Choi, Young Joo Sohn, Ye Kyung Kim, Kyung Min Kim, Ji Young Park, Hyun Mi Kang, In Ae Yoon, Jae Hong Choi, Chi Eun Oh, Eun Young Cho, Hyunju Lee, Eun Hwa Choi
Summary: The study in Korea between 2014 and 2019 found that the carriage rates of Streptococcus pneumoniae and non-typeable Haemophilus influenzae in children varied, with a significant increase in carriage of serotype 23A of S. pneumoniae. Children who had received pneumococcal conjugate vaccines had a lower risk of vaccine-type carriage. Continuous surveillance is necessary to evaluate the long-term effects of PCVs on the carriage dynamics of these bacteria.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Immunology
Kaunda Yamba, Evans Mpabalwani, Ruth Nakazwe, Evans Mulendele, Goitom Weldegebriel, Jason M. Mwenda, Reggis Katsande, Linda de Gouveia, Elizabeth Chizema-Kawesha, Raphael Chanda, Belem Matapo, James C. L. Mwansa, Chileshe Lukwesa-Musyani
Summary: In Zambia, the main pathogens causing bacterial meningitis in children under 5 years are Streptococcus pneumoniae, Haemophilus influenzae, and Neisseria meningitidis, with some strains showing resistance to penicillin. The introduction of PCV10 vaccine has led to a decrease in pneumococcal meningitis and the proportion of PVC10 serotypes after vaccination, but there is a concern about serotype replacement and penicillin resistance that require continued surveillance for policy-making.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Review
Microbiology
Celine Betti, Pietro Camozzi, Viola Gennaro, Mario G. Bianchetti, Martin Scoglio, Giacomo D. Simonetti, Gregorio P. Milani, Sebastiano A. G. Lava, Alessandra Ferrarini
Summary: This study documented cases of leukocytoclastic small-vessel vasculitis in children following symptomatic diseases caused by Mycoplasma pneumoniae infection. Patients with systemic vasculitis and skin-limited vasculitis did not significantly differ in terms of gender and age, and most patients recovered within 12 weeks.
Article
Medicine, General & Internal
Maria Scaturro, Luna Girolamini, Maria Rosaria Pascale, Marta Mazzotta, Federica Marino, Giulia Errico, Monica Monaco, Antonietta Girolamo, Maria Cristina Rota, Maria Luisa Ricci, Sandra Cristino
Summary: This article describes a rare case of pulmonary Klebsiella pneumoniae-Legionella pneumophila coinfection in a man with chronic renal disease who had undergone a double kidney transplantation. Cases of Legionnaires' disease with a 14-day incubation period are seldom documented. Despite a lengthy hospitalization, typing of clinical and environmental L. pneumophila strains revealed that the patient's home water distribution system was the source of infection, emphasizing the concealed risk of Legionella contamination in households, particularly for immunocompromised individuals.
FRONTIERS IN MEDICINE
(2022)
Article
Medicine, General & Internal
Jocelyn Chan, Heather F. Gidding, Christopher C. Blyth, Parveen Fathima, Sanjay Jayasinghe, Peter B. McIntyre, Hannah C. Moore, Kim Mulholland, Cattram D. Nguyen, Ross Andrews, Fiona M. Russell
Summary: This study found substantial indirect protection at lower levels of PCV coverage, challenging the traditional assumption that high PCV coverage is necessary. Understanding the association between PCV coverage and indirect protection is crucial for future vaccination plans.
Article
Microbiology
Gang Wang, Jean-Michel Brunel, Jean-Michel Bolla, Francoise Van Bambeke
Summary: The study found that the polyaminoisoprenyl potentiator NV716 significantly reduced the minimum inhibitory concentration of multiple antibiotics against Pseudomonas aeruginosa and improved their intracellular activity. Compared to other two potentiators, NV716 can resensitize P. aeruginosa to antibiotics both extracellularly and intracellularly, indicating a complex mode of action.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
Herve Poilvache, Albert Ruiz-Sorribas, Olivier Cornu, Francoise Van Bambeke
Summary: The study found that a tri-enzymatic cocktail could effectively disperse biofilms, and the combination with antibiotics resulted in a synergistic reduction of bacteria count in Staphylococcus aureus, Staphylococcus epidermidis, and Escherichia coli biofilms.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
Carine Yehouenou, Bert Bogaerts, Kevin Vanneste, Nancy H. C. Roosens, Sigrid C. J. De Keersmaecker, Kathleen Marchal, Dissou Affolabi, Reza Soleimani, Hector Rodriguez-Villalobos, Francoise Van Bambeke, Olivia Dalleur, Anne Simon
Summary: This study reports the first detection of a NDM-1-producing Acinetobacter baumannii strain in Benin, which exhibits high levels of drug resistance. The strain carries numerous antibiotic resistance genes, with the NDM-1 gene located in a Tn125 transposon on a plasmid, indicating a high potential risk of horizontal gene transfer.
ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
(2021)
Article
Microbiology
Albert Ruiz-Sorribas, Herve Poilvache, Francoise Van Bambeke
Summary: This study investigated the activity of different drugs against interkingdom biofilms using a three-species biofilm model. The results showed limited effectiveness of the drugs when used alone, but increased effects were observed when the drugs were used in combination, particularly against three-species biofilms. Additionally, different antibiotics showed better efficacy against different bacterial species. These findings were further confirmed by confocal microscopy images.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Chemistry, Medicinal
Gang Wang, Jean-Michel Brunel, Hector Rodriguez-Villalobos, Jean-Michel Bolla, Francoise Van Bambeke
Summary: NV716 can enhance the activity of antibiotics against difficult-to-treat Gram-negative bacteria by interacting with the bacterial outer membrane, resulting in increased accumulation of antibiotics inside bacteria. It can also increase the activity of antibiotics against intracellular infection and biofilms, without toxicity to eukaryotic cells at sub-MIC concentrations.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Infectious Diseases
Angele Modupe Dohou, Valentina Oana Buda, Loconon Achille Yemoa, Severin Anagonou, Francoise Van Bambeke, Thierry Van Hees, Francis Moise Dossou, Olivia Dalleur
Summary: The excessive use and misuse of antibiotics have a significant impact on the global numbers of antibiotic-resistant pathogens and commensal bacteria. In Benin, there are various problems with antibiotic use during caesarean sections, highlighting the need for a national policy to improve antibiotic usage.
Article
Infectious Diseases
Angele Modupe Dohou, Valentina Oana Buda, Severin Anagonou, Francoise Van Bambeke, Thierry Van Hees, Francis Moise Dossou, Olivia Dalleur
Summary: A low adherence to recommendations on antibiotic prophylaxis has been reported worldwide. In Benin, despite the provision of free kits containing necessary supplies and antibiotics for prophylaxis during cesarean sections, the level of antibiotic prophylaxis achievement remains low. A study in three hospitals found that healthcare professionals had limited knowledge and various determinants, such as suboptimal patient health status, low confidence in antibiotics, disagreement with the policy, inappropriate infrastructures and limited financial resources, poor management of the policy at the central level, and patient refusal to buy antibiotics, explain the poor practices. Policymakers must consider these determinants to improve antibiotic prophylaxis and reduce antimicrobial resistance.
Article
Infectious Diseases
Perrin Ngougni Pokem, Xavier Wittebole, Christine Collienne, Hector Rodriguez-Villalobos, Paul M. Tulkens, Laure Elens, Francoise Van Bambeke, Pierre-Francois Laterre
Summary: This study investigated the pharmacokinetics of temocillin in plasma and ascitic fluid, and performed dosing simulations. The current dosing regimen achieved the target concentration in plasma, but not in ascitic fluid. Adjusting the dosing regimen might allow for target concentration attainment in ascitic fluid, but further evaluation is needed for safety and efficacy.
Review
Pharmacology & Pharmacy
David De Bels, Evelyne Maillart, Francoise Van Bambeke, Sebastien Redant, Patrick M. Honore
Summary: Invasive candidiasis or candidemia is a severe global infection with high mortality, and therapeutic failure is common due to the emergence of resistant strains and new species. This review discusses the current antifungal drugs used for candidiasis and the advancements made in new formulations, derivatives, and repurposed drugs. The results from ongoing and future clinical trials will be crucial in determining the efficacy and interest of these drugs in the treatment of candidiasis.
EXPERT OPINION ON EMERGING DRUGS
(2022)
Article
Infectious Diseases
Maria Angeles Melendez-Carmona, Mikel Mancheno-Losa, Albert Ruiz-Sorribas, Irene Munoz-Gallego, Esther Viedma, Fernando Chaves, Francoise Van Bambeke, Jaime Lora-Tamayo
Summary: There is significant variability in the response of biofilm-embedded Staphylococcus aureus to levofloxacin and rifampicin. A lower range response, combined with other factors, could be responsible for treatment failure.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Biology
Gang Wang, Jean-Michel Brunel, Matthias Preusse, Negar Mozaheb, Sven D. Willger, Gerald Larrouy-Maumus, Pieter Baatsen, Susanne Haeussler, Jean-Michel Bolla, Francoise Van Bambeke
Summary: The polyaminoisoprenyl compound NV716 re-sensitizes Pseudomonas aeruginosa to antibiotics by permeabilizing the outer membrane and increases the activity of antibiotics on biofilms.
COMMUNICATIONS BIOLOGY
(2022)
Correction
Microbiology
Albert Ruiz-Sorribas, Herve Poilvache, Nur Hidayatul Nazirah Kamarudin, Annabel Braem, Francoise Van Bambeke
MICROBIOLOGY SPECTRUM
(2023)
Editorial Material
Infectious Diseases
Francoise Van Bambeke, Sebastian Wicha, Paul M. Tulkens, Markus Zeitlinger
Article
Microbiology
Frederic Peyrusson, Tiep Khac Nguyen, Tome Najdovski, Francoise Van Bambeke
Summary: This study investigates the effect of reactive oxygen species (ROS) on the dormancy depth of intracellular S. aureus persisters in host cells. It is found that host ROS can induce variable dormant states in persisters and the level of oxidative stress determines the capacity for resuscitation. The results highlight the importance of understanding the interplay between ROS, ATP depletion, and dormancy state in S. aureus infections.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Albert Ruiz-Sorribas, Herve Poilvache, Nur Hidayatul Nazirah Kamarudin, Annabel Braem, Francoise Van Bambeke
Summary: Biofilms are resistant to antimicrobials, but hydrolytic enzymes can reduce their biomass. Subtilisin A and lyticase are the most effective enzymes against interkingdom biofilms. Combining enzymes with antimicrobials further reduces the biomass.
MICROBIOLOGY SPECTRUM
(2022)
