期刊
CELL PROLIFERATION
卷 54, 期 9, 页码 -出版社
WILEY
DOI: 10.1111/cpr.13101
关键词
cisplatin; G-quadruplex; inflammatory microenvironment; osteosarcoma; TMPyP4
类别
资金
- Wenzhou Science and Technology Key Project [2018ZY009]
- Health Commission of Zhejiang Province [2019KY646]
- Wenzhou Basic Medical and Health Technology Project [Y20180177]
- Zhejiang Provincial Key Scientific Project [2018C03068, 2021C03041]
- National Natural Science Foundation of China [21701194, 81930108]
The study showed that TMPyP4 is more suitable for osteosarcoma treatment than cisplatin. TMPyP4 induced the formation of human telomeres and FAK G-quadruplex in vitro and in vivo, and suppressed ALT activity and FAK-related cell migration in OS cells in the inflammatory microenvironment.
Objective Osteosarcoma (OS) is characterized by high levels of the tumour-associated inflammatory microenvironment. Moreover, in approximately 60% of OS, telomere length is maintained by alternative lengthening of telomeres (ALT) pathway. Whether the ALT pathway can be exploited for OS therapeutic treatment and how the OS inflammatory microenvironment influences the anti-cancer drug effect remains unknown. Here, we examined the biological effects of TMPyP4 and cisplatin in the inflammatory microenvironment of OS cells. Materials and methods Immunofluorescence in situ hybridization (IF-FISH) and C-circle experiments were used to detect the G-quadruplex and ALT activity. The redox potential of single guanine, G-quadruplex and G-quadruplex/TMPyP4 was evaluated by the lowest unoccupied molecular orbital energy (LUMO), zeta potential and cyclic voltammetry. Cell viability, flow cytometry and apoptosis, Western blot, comet assay, adhesion, transwell and scratch experiments were performed to compare the anti-tumour proliferation and migration effects of TMPyP4 and cisplatin in the inflammatory microenvironment. Results This study indicated that compared with cisplatin, TMPyP4 could induce the formation of human telomeres and FAK G-quadruplex in vitro and in vivo, and TMPyP4-treated OS cells showed fewer extrachromosomal C-circles and fewer ALT-associated promyelocytic leukaemia bodies. Consequently, the ALT activity and FAK-related cell migration were suppressed by TMPyP4. Mechanistically, the formation of G-quadruplex resulted in both lower redox potential than G within the genome and FAK transcription inhibition, and TMPyP4 could enhance this phenomenon, especially in the inflammatory microenvironment. Conclusions Our results reveal that TMPyP4 is more suitable for OS treatment than cisplatin.
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