期刊
CELL METABOLISM
卷 33, 期 9, 页码 1869-+出版社
CELL PRESS
DOI: 10.1016/j.cmet.2021.07.018
关键词
-
资金
- Margareta af Uggla's foundation
- Knut & Alice Wallenberg's foundation
- Swedish Research Council
- ERC-SyG SPHERES [856404]
- NovoNordisk Foundation [NNF15CC0018486, NNF15SA0018346, 0064142, NNF20OC0061149]
- CIMED
- Swedish Diabetes Foundation
- Stockholm County Council
- Erling-Persson Family Foundation [140604]
- Strategic Research Program in Diabetes at Karolinska Institutet
- European Research Council (ERC) [856404] Funding Source: European Research Council (ERC)
By studying the composition and structure of WAT, we identified that human WAT is composed of three classes of mature adipocytes, only one of which is insulin responsive.
The contribution of cellular heterogeneity and architecture to white adipose tissue (WAT) function is poorly understood. Herein, we combined spatially resolved transcriptional profiling with single-cell RNA sequencing and image analyses to map human WAT composition and structure. This identified 18 cell classes with unique propensities to form spatially organized homo-and heterotypic clusters. Of these, three constituted mature adipocytes that were similar in size, but distinct in their spatial arrangements and transcriptional profiles. Based on marker genes, we termed these Adipo(LEP), Adipo(PLIN), and Adipo(SAA). We confirmed, in independent datasets, that their respective gene profiles associated differently with both adipocyte and whole-body insulin sensitivity. Corroborating our observations, insulin stimulation in vivo by hyperinsulinemic-euglycemic clamp showed that only Adipo(PLIN) displayed a transcriptional response to insulin. Altogether, by mining this multimodal resource we identify that human WAT is composed of three classes of mature adipocytes, only one of which is insulin responsive.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据