Fluorescent labeling affected the structural/conformational properties of arabinoxylans

Three fluorescent-labeling methods, including DMSO/DBTD, reductive amination and fluoresceinamine (FA), were applied to label arabinoxylan (AX). The conjugates were named AF, ATF and ACF, respectively. Compared to AX, the water solubility of AF significantly decreased while that of ATF significantly increased (p < 0.05); both showed decreased solubility with increasing degree of substitution (DS). However, AX was not successfully labeled by the FA method due to poor solubility. The structural and conformational properties of the AF and ATF conjugates under different DS were compared. Results showed that fluorochrome (FITC) was successfully grafted onto AX molecule by using the DMSO/DBTD and reductive amination methods. A slight increased Mw was noticed for both AF and ATF. AF existed in a more compact conformation, while the chain conformation of ATF was more elongated at a lower DS (1.15 %) and more compact at a higher DS (1.34 %).

Automated summary

A study applied three fluorescent-labeling methods to label arabinoxylan, with two methods successfully grafting the fluorochrome onto the molecule and comparing structural properties under different degrees of substitution, while the third method failed due to solubility issues. The conjugates showed different solubilities and conformations depending on the labeling method and degree of substitution.