Article
Genetics & Heredity
Xiangchun Pan, Qingnan Li, Danxia Chen, Wentao Gong, Nian Li, Yao Jiang, Hao Zhang, Yaosheng Chen, Xiaolong Yuan
Summary: This study investigated the dynamics of alternative splicing (AS) and differentially expressed AS (DEAS) events in the hypothalamus-pituitary-ovary (HPO) axis across pre-, in-, and post-puberty stages in pigs. The DEAS events were found to be associated with various signaling pathways and specific genes, shedding new light on the molecular mechanisms underlying the timing of puberty in mammals.
FRONTIERS IN GENETICS
(2021)
Review
Biochemistry & Molecular Biology
Jinbi Zhang, Caixia Wang, Chao Jia, Yi Zhang, Xinxin Qing, Yuge Zhang, Jingge Liu, Shiyong Xu, Zengxiang Pan
Summary: This review summarizes the characteristics, classification, biogenesis and elimination, detection and confirmation, and functions of circular RNAs (circRNAs), with a focus on the research advances in the mammalian ovary and reproductive processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
Qingnan Li, Xiangchun Pan, Nian Li, Wentao Gong, Yaosheng Chen, Xiaolong Yuan
Summary: The study profiled the changes and functions of circRNAs in the hypothalamus during the onset of puberty, revealing enrichment in signaling pathways related to neurotrophins, progesterone-mediated oocyte maturation, oocyte meiosis, insulin, ErbB, and mTOR, all of which are suggested to play a role in regulating puberty timing.
Review
Biochemical Research Methods
Jiashu Liu, Cui-Xiang Lin, Xiaoqi Zhang, Zongxuan Li, Wenkui Huang, Jin Liu, Yuanfang Guan, Hong-Dong Li
Summary: Alternative splicing (AS), a key pathway for transcriptional regulation, has been shown to be associated with complex diseases. Computational approaches for detecting disease-associated AS events have been developed. This review discusses the metrics used for characterizing AS events quantitatively. It also reviews and discusses three types of methods for detecting disease-associated splicing events: differential splicing analysis, aberrant splicing detection, and splicing-related network analysis. Additionally, methods for detecting genetic variants that potentially regulate splicing are described. Experimental comparisons are conducted to illustrate the performance of each method. The limitations of these methods are discussed, as well as potential ways to address them. This review aims to provide a systematic understanding of computational approaches for analyzing disease-associated splicing.
BRIEFINGS IN BIOINFORMATICS
(2023)
Review
Oncology
Dan Cheng, Jing Wang, Zigang Dong, Xiang Li
Summary: Noncoding RNAs, including circRNAs, play an important role in various diseases, and their potential as biomarkers for cancer prognosis and diagnosis is discussed. The features, classification, and analysis methods of circRNAs are also summarized in this review.
CANCER CELL INTERNATIONAL
(2021)
Article
Agriculture, Dairy & Animal Science
Aiju Liu, Xiaoyong Chen, Menghe Liu, Limeng Zhang, Xiaofei Ma, Shujun Tian
Summary: Litter size is an important trait in meat sheep breeding, and this study identified differentially expressed circRNAs in the ovaries of Hanper sheep with high and low fertility during different phases of the estrous cycle. MAPK signaling pathways were found to play a key role in regulating litter size in Hanper sheep. Additionally, AKT3 was identified as a core gene involved in this regulation.
Review
Endocrinology & Metabolism
Panyisha Wu, Moya Zhang, Nicholas J. G. Webster
Summary: Alternative RNA splicing is an important biological process that has been linked to various clinical syndromes, but its applicability to common diseases is still uncertain. This review focuses on the changes in alternative RNA splicing in fatty liver disease and the role of splicing regulation in disease progression.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yingjun Chen, Qinghua Liu, Ruiyan Liu, Chan Yang, Xiaodong Wang, Zaohong Ran, Shanshan Zhou, Xiang Li, Changjiu He
Summary: This study in a mouse model revealed that early folliculogenesis requires a minimum of two weeks, with different stages showing specific characteristics such as oocyte size at Type 4-5 stage and theca folliculi formation at PD10-12. The study also highlighted the importance of the PD22-23 period for studying puberty initiation, providing valuable insights into the biological events of this process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Carmelo Gurnari, Simona Pagliuca, Valeria Visconte
Summary: Alternative RNA splicing is an essential physiological function that plays a crucial role in cellular development. RNA splicing dysfunction has been implicated in the development of several cancers, including myeloid malignancies. Cancer cells exhibit marked gene expression alterations, including different usage of AS isoforms, potentially causing tissue-specific effects and disruptions of downstream pathways.
Review
Immunology
Jianli Zhou, Qiao Zhang, Yuzhen Zhao, Yuchen Song, Yanan Leng, Moxian Chen, Shaoming Zhou, Zhaoxia Wang
Summary: Inflammatory bowel disease (IBD) is a group of chronic diseases including Crohn's disease and ulcerative colitis, which have a global impact on both children and adults. The burden of IBD is increasing worldwide, and the costs associated with it are high. The pathogenesis of IBD is still unclear, but is believed to be related to environmental factors, gut microbiota, immune imbalance, and genetic susceptibility. This article reviews the research progress on alternative splicing events, splicing factors, and splicing mutations associated with IBD.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Oncology
Anthony J. Murphy, Alex H. Li, Peichao Li, Hong Sun
Summary: Alternative splicing plays a crucial role in diversifying the proteome and has significant implications in cancer development. Recent studies have highlighted the important roles of SR proteins in cancer progression. Understanding the spliceosome and its relationship to cancer has opened up new opportunities for therapeutic interventions based on alternative splicing.
FRONTIERS IN ONCOLOGY
(2022)
Article
Cell Biology
Pe'era Wasserzug Pash, Gilad Karavani, Eli Reich, Lital Zecharyahu, Zehava Kay, Dvora Bauman, Talya Mordechai-Daniel, Tal Imbar, Michael Klutstein
Summary: Pre-pubertal oocytes are dormant and do not undergo meiotic divisions naturally. Pubertal transition triggers molecular changes and epigenetic events in oocytes. Our study shows that pubertal transition leads to reorganization of oocyte chromatin and elevation of histone methylation levels. Understanding these changes is important for developing methods to mature pre-pubertal oocytes for fertility preservation in young cancer survivors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Genetics & Heredity
Qi Liu, Leiming Fang, Chengjun Wu
Summary: Alternative splicing of pre-mRNA is a key mechanism for increasing protein complexity, leading to diversity of transcriptomes and proteomes in a tissue-specific manner. Changes and errors in splicing regulation caused by splicing factors are closely related to various diseases. Understanding cellular factors that mediate alternative splicing regulation is a potential avenue for developing specific drugs targeting the dynamic RNA splicing process.
Article
Cell Biology
Zhe Liu, Wei Wang, Xinru Li, Xiujuan Zhao, Hongyu Zhao, Wuritu Yang, Yongchun Zuo, Lu Cai, Yongqiang Xing
Summary: Alternative splicing plays an important role in zebrafish embryo development, especially during the maternal-to-zygotic transition process where it is highly abundant and dynamic. Splicing factors are expressed with developmental stage specificity, with a higher expression during the maternal-to-zygotic transition. The inclusion level of alternative splicing events performs well in cluster analysis and can be used as a novel parameter.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Oncology
Jiawei Ouyang, Yijie Zhang, Fang Xiong, Shanshan Zhang, Zhaojian Gong, Qijia Yan, Yi He, Fang Wei, Wenling Zhang, Ming Zhou, Bo Xiang, Fuyan Wang, Xiaoling Li, Yong Li, Guiyuan Li, Zhaoyang Zeng, Can Guo, Wei Xiong
Summary: This article introduces the definition of alternative splicing in eukaryotes and its importance in cancer development. Alternative splicing is a transcription process that allows coding genes to be translated into multiple proteins with different functions. Alterations in splicing mechanisms may lead to changes in the splicing patterns of cancer-related genes.
AMERICAN JOURNAL OF CANCER RESEARCH
(2021)