Article
Biochemistry & Molecular Biology
Fatih Karadeniz, Jung Hwan Oh, Mi Soon Jang, Youngwan Seo, Chang-Suk Kong
Summary: Bone marrow adiposity is a complication in osteoporotic patients, and phytochemicals can alleviate it. This study evaluated the effect of libanoridin on the adipogenic differentiation of bone marrow-derived mesenchymal stromal cells. The results showed that libanoridin inhibited adipogenic differentiation through suppressing MAPK-mediated PPAR gamma signaling. Future studies will further explore the anti-adipogenic effects of libanoridin in vivo and elucidate its action mechanism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Valentina Orticelli, Andrea Papait, Elsa Vertua, Patrizia Bonassi Signoroni, Pietro Romele, Lorena Di Pietro, Marta Magatti, Luciana Teofili, Antonietta Rosa Silini, Ornella Parolini
Summary: This study investigated the potential of human term placenta-derived amniotic membrane MSCs (hAMSC) to support the ex vivo expansion of HSC and progenitor cells. The results suggest that hAMSCs are a valuable alternative to BM-MSC for enhancing the proliferation and maintaining stemness of CB-HSC, and can improve the colony forming unit ability and long-term culture initiating cells ability, addressing the need for high HSC content in CB units available for transplantation.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Review
Cell Biology
Yuan Liu, Christina Holmes
Summary: This review explores the tissue regeneration potential of EVs isolated from bone marrow and adipose tissue, the two most common clinical sources of adult MSCs, focusing on their angiogenic, osteogenic, and immunomodulatory potentials. Other important issues in the development of MSC-derived EV based therapies are also discussed.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Madhan Jeyaraman, Shiva Kumar Bingi, Sathish Muthu, Naveen Jeyaraman, Rathinavelpandian Perunchezhian Packkyarathinam, Rajni Ranjan, Shilpa Sharma, Saurabh Kumar Jha, Manish Khanna, Sree Naga Sowndary Rajendran, Ramya Lakshmi Rajendran, Prakash Gangadaran
Summary: Human bone marrow is a promising source of mesenchymal stromal cells (MSCs) for regenerative procedures involving cartilage and bone. However, the low proportion of MSCs in the bone marrow requires careful harvesting and processing techniques. There is currently a lack of consensus regarding the variables in the harvesting and processing technique of bone marrow aspiration concentrate (BMAC) and their impact on MSC yield.
BIOENGINEERING-BASEL
(2022)
Article
Biology
Manali Jain, Neeta Singh, Raunaq Fatima, Aditya Nachanekar, Mandakini Pradhan, Soniya Nityanand, Chandra Prakash Chaturvedi
Summary: This study compares the growth and cardiomyogenic potential of AF-MSCs from congenital heart defective fetuses (ICHD) with normal fetuses. It finds that ICHD AF-MSCs have growth defects, increased senescence and DNA damage, and decreased expression of proteins necessary for heart development. These findings highlight the possible contribution of defects in AF-MSCs to congenital heart defects and improper heart development.
Article
Cell & Tissue Engineering
Min-Kyoung Song, Hyun Jin Sun, Sun Wook Cho
Summary: A study evaluated the effects of a conditioned medium of amniotic fluid-derived stromal cells (AFSC-CM) on bone metabolism. The results showed that AFSC-CM increased bone mass and enhanced bone turnover in mice. Additionally, AFSC-CM increased osteoclast differentiation and promoted endothelial cell migration and tube formation. The study suggests that AFSC-CM can have a bone anabolic effect by altering the bone marrow microenvironment, including angiogenesis and precursor expansion.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2022)
Article
Cell Biology
Domenico Aprile, Nicola Alessio, Ibrahim H. Demirsoy, Tiziana Squillaro, Gianfranco Peluso, Giovanni Di Bernardo, Umberto Galderisi
Summary: The study demonstrated that multi-lineage stem cells can be isolated from a mouse model, offering a valid alternative for disease research and cell therapy, and showing potential applications for these cells.
Article
Cell & Tissue Engineering
Wei Lee, Li-Tzu Wang, Men-Luh Yen, Pei-Ju Hsu, Yu-Wei Lee, Ko-Jiunn Liu, Kuo- Lin, Yu-Wen Su, Huey-Kang Sytwu, B. Linju Yen
Summary: In vitro, placental MSCs significantly inhibit proliferation and differentiation of stimulated human peripheral B cell populations, while BM-MSCs do not. Additionally, P-MSCs increase all subsets of regulatory B cells (Bregs) producing IL-10, while BM-MSCs do not.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Enrico Ragni, Carlotta Perucca Orfei, Marco Vigano, Federico Valli, Laura de Girolamo
Summary: Bone-marrow-derived stromal cells (BMSCs) are a promising therapeutic option for osteoarthritis (OA) due to their regenerative features. Extracellular vesicles (EVs) released by BMSCs contain miRNAs that play a crucial role in their therapeutic potential. This study aims to identify reliable reference genes (RGs) for studying miRNAs in BMSC-EVs cultured under standard or OA synovial fluid (OA-SF). The results suggest that miR-23a-3p and miR-221-3p are the best candidates, and miR-24-3p is the most stable RG for comparing EVs content.
Review
Cell & Tissue Engineering
Francesca Veronesi, Melania Maglio, Deyanira Contartese, Lucia Martini, Aurelio Muttini, Milena Fini
Summary: Musculoskeletal regenerative medicine focuses on using cell therapy to heal damaged tissues such as bone, cartilage, and tendons. Different cell types, including SVF and AECs, are considered promising alternatives to traditional options like ADSCs and BMSCs, showing potential in promoting cell and tissue regeneration.
STEM CELLS INTERNATIONAL
(2021)
Article
Cell Biology
Wu Duan, Huajie Zou, Nan Zang, Dongxia Ma, Bo Yang, Lin Zhu
Summary: Bone marrow adipose tissue (MAT) can have both local and systemic effects on metabolic homeostasis. The effects of metformin, a first-line drug for type 2 diabetes, on MAT and bone marrow mesenchymal stem cell (BM-MSC) differentiation are conflicting. In vivo and in vitro experiments showed that metformin increased MAT in mice tibia and promoted both osteogenic and lipogenic gene expression in mouse bone marrow. However, metformin inhibited lipogenesis and promoted osteogenesis of MSC in vitro. The increased MAT may be attributed to the filling of adipose tissue after metformin-induced apoptosis of bone marrow stromal cells.
Article
Cell & Tissue Engineering
Behnoosh Tayebi, Mahnaz Babaahmadi, Mohammad Pakzad, Mostafa Hajinasrollah, Farhad Mostafaei, Shahrbanoo Jahangiri, Amir Kamali, Hossein Baharvand, Mohamadreza Baghaban Eslaminejad, Seyedeh-Nafiseh Hassani, Ensiyeh Hajizadeh-Saffar
Summary: Mesenchymal stromal cells (MSCs) have shown potential in treating inflammatory and non-inflammatory diseases, but their clinical applications require strict evaluations and monitoring to ensure compliance with safety standards. This study aimed to assess the potential toxicity effects of cryopreserved human bone marrow-derived clonal MSCs in animal models during different time periods.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Oncology
Rafaela Rossetti, Felipe Augusto Ros, Lucas Eduardo Botelho de Souza, Juliana de Matos Maconetto, Pericles Natan Mendes da Costa, Fernanda Ursoli Ferreira, Josiane Serrano Borges, Julianne Vargas de Carvalho, Nayara Patricia Morotti, Simone Kashima, Dimas Tadeu Covas
Summary: The bone marrow microenvironment may contain two distinct populations of mesenchymal stromal cells (MSC), one located perivascularly and the other in the endosteum. Recent research has focused on better understanding these MSC subsets in order to improve their clinical applications. Studies indicate that compact bone is a rich source of MSC and offers lower risk of contamination by hematopoietic cells. Compact bone-derived MSC also show higher osteogenic potential compared to bone marrow-derived MSC. These findings suggest the presence of functionally different MSC populations in bone marrow and compact bone.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Gloria Bua, Pasquale Marrazzo, Elisabetta Manaresi, Chiara Gamberini, Laura Bonsi, Francesco Alviano, Giorgio Gallinella
Summary: Both BM-MSCs and FM-MSCs can be infected by B19V, although they cannot support viral replication, they allow persistence of the virus in the infected cultures. Further studies are needed to understand the potential role of MSCs in B19V transmission and the conditions that can favor a potential reactivation of the virus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Alexey Yu. Lupatov, Roza Yu. Saryglar, Valentina V. Vtorushina, Rimma A. Poltavtseva, Oxana A. Bystrykh, Vladimir D. Chuprynin, Lyubov V. Krechetova, Stanislav V. Pavlovich, Konstantin N. Yarygin, Gennady T. Sukhikh
Summary: A comparative analysis was conducted on the cell surface markers and immunological properties of cell cultures from normal endometrium and endometrioid heterotopias in women with extragenital endometriosis. Despite similar phenotypes, stromal cells from endometrioid heterotopias showed enhanced pro-inflammatory and immunosuppressive activities compared to those from eutopic endometrium. The results suggest that these differences may impact the pathogenesis and progression of the disease.
Article
Pediatrics
Pier-Angelo Tovo, Anna Opramolla, Antonio Pizzol, Giulia Calosso, Valentina Dapra, Ilaria Galliano, Cristina Calvi, Michele Pinon, Fabio Cisaro, Caterina Rigazio, Pier Luigi Calvo, Massimiliano Bergallo
Summary: This study found that the transcription levels of pol genes of HERV-H, -K, and -W in WBCs from celiac patients were significantly higher than those in the control group, suggesting that these HERVs may play a role in the development of celiac disease.
EUROPEAN JOURNAL OF PEDIATRICS
(2021)
Article
Biochemistry & Molecular Biology
Stefano Canosa, Katia Mareschi, Elena Marini, Andrea Roberto Carosso, Sara Castiglia, Deborah Rustichelli, Ivana Ferrero, Gianluca Gennarelli, Benedetta Bussolati, Alberto Nocifora, Valentina Asnaghi, Massimiliano Bergallo, Ciro Isidoro, Chiara Benedetto, Alberto Revelli, Franca Fagioli
Summary: The study compared two different endometrial sampling methods and validated a novel xeno-free method for culturing human E-MSCs. Results showed that VABRA endometrial sampling could be an alternative to surgical curettage, providing efficient isolation and culture of E-MSCs in Good Manufacturing Practice compliance conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Katia Mareschi, Alessia Giovanna Santa Banche Niclot, Elena Marini, Elia Bari, Luciana Labanca, Graziella Lucania, Ivana Ferrero, Sara Perteghella, Maria Luisa Torre, Franca Fagioli
Summary: This study modified the type of culture supplement used in the production of freeze-dried mesenchymal stem cell (MSC)-secretome (lyo-secretome), and found that changing the supplement type only resulted in minimal changes in product composition without affecting potency, validating the new process according to GMP standards.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Katia Mareschi, Elena Marini, Alessia Giovanna Santa Banche Niclot, Marta Barone, Giuseppe Pinnetta, Aloe Adamini, Manuela Spadea, Luciana Labanca, Graziella Lucania, Ivana Ferrero, Franca Fagioli
Summary: This study demonstrates that the use of HPL-S is an effective alternative for MSC production under GMP conditions. The protein and growth factor content in HPL-S is comparable to that of HPL-E. Furthermore, MSCs derived from HPL-S show lower HLA-DR expression in immunophenotypic analysis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, General & Internal
Francesco Savino, Valentina Dapra, Andrea Savino, Cristina Calvi, Paola Montanari, Ilaria Galliano, Massimiliano Bergallo
Summary: This study aimed to measure IFN-gamma and IDO1 values in infants' White blood cells during respiratory tract infections. The results showed a significant reduction in IFN-gamma expression in infants with RSV bronchiolitis, compared with healthy controls, while there was no significant difference in IDO1 expression between the two groups. New investigations focusing on RSV-infected subjects at different stages of infancy could provide further insights into the relationship between IDO1 expression and age.
Article
Allergy
Pier-Angelo Tovo, Giovanna Monti, Valentina Dapra, Paola Montanari, Cristina Calvi, Carla Alliaudi, Allegra Sardo, Ilaria Galliano, Massimiliano Bergallo
Summary: This study found that the transcription levels of multiple HERV genes were significantly enhanced in patients with food allergies, while the mRNA levels of TRIM28 and SETDB1 were also significantly upregulated. This suggests that HERVs, as well as TRIM28 and SETDB1, may play important roles in the development of food allergies.
CLINICAL AND TRANSLATIONAL ALLERGY
(2022)
Article
Virology
Ilaria Galliano, Valentina Dapra, Maddalena Dini, Martina Gavatorta, Allegra Sardo, Giada Lo Curcio, Massimiliano Bergallo
Summary: This study aims to quantify Rotavirus in fecal samples of children with acute gastroenteritis using taqman real-time PCR, in accordance with the program of reduction of drug-resistance and use of antibiotics. The results showed that Rotavirus was detectable in 34.7% of children hospitalized for acute gastroenteritis, a rate similar to previous studies conducted elsewhere.
Review
Oncology
Alessandro Godono, Francesco Felicetti, Alessio Conti, Marco Clari, Margherita Dionisi-Vici, Filippo Gatti, Catalina Ciocan, Tommaso Pinto, Emanuela Arvat, Enrico Brignardello, Franca Fagioli, Enrico Pira
Summary: This study assessed the prevalence of employment among childhood cancer survivors (CCS) and found that two-thirds of them were employed worldwide. The study also identified different employment rates based on socio-demographic and clinical factors, and highlighted the importance of designing targeted interventions to promote employment in this population.
Article
Oncology
Margherita Dionisi-Vici, Alessandro Godono, Anna Castiglione, Filippo Gatti, Nicoletta Fortunati, Marco Clari, Alessio Conti, Giulia Zucchetti, Eleonora Biasin, Antonella Varetto, Enrico Pira, Franca Fagioli, Enrico Brignardello, Francesco Felicetti
Summary: This study aims to describe the occupational status of long-term childhood cancer survivors (CCS) and evaluate the association between late effects and unemployment and job satisfaction. The results show that severe late effects have a negative impact on occupation and worsen the perception of satisfaction with the economic situation of unemployed CCS. Routine follow-up care for CCS should include monitoring their socioeconomic development and providing interventions to help them obtain suitable jobs.
Article
Pharmacology & Pharmacy
Alessia Giovanna Santa Banche Niclot, Elena Marini, Ivana Ferrero, Francesco Barbero, Elena Rosso, Ivana Fenoglio, Alessandro Barge, Augusto Pessina, Valentina Cocce, Francesca Paino, Katia Mareschi, Franca Fagioli
Summary: This study demonstrated that the conditioned medium isolated from MSCs loaded with PTX exhibited strong cytotoxic effects on OS cell lines. Additionally, the presence of nanoparticles with a size similar to extracellular vesicles and minimal doses of PTX were detected in the secretome.
Article
Psychology, Multidisciplinary
Giulia Zucchetti, Giorgia Gamberini, Sabrina Ciappina, Celeste Cagnazzo, Federica Ricci, Stefano Vallero, Paola Quarello, Paola Peretta, Franca Fagioli
Summary: This study aims to describe an innovative prospective observational study protocol for evaluating and monitoring the neuropsychological outcomes of children and adolescents affected by brain tumors. It involves assessing patients at three different time points using standardized neuropsychological tests, allowing clinicians to provide appropriate rehabilitation and educational programs from the early stages.
BEHAVIORAL SCIENCES
(2023)
Article
Pediatrics
Giulia Zucchetti, Sabrina Ciappina, Cristiana Risso, Alice Malabaila, Sara Racalbuto, Elena Longo, Margherita Dionisi Vici, Marina Bertolotti, Paola Quarello, Franca Fagioli
Summary: This study investigated the feelings of children and adolescents with acute or chronic illness towards the COVID-19 pandemic in Italy, and compared their experiences with children without illness. The results showed that participants, regardless of their health condition, felt fear of the virus and potential infection for themselves and their families, but experienced less interference with daily functioning. The fragile group demonstrated greater resilience towards the pandemic situation compared to the low-risk group, and some differences were found within the fragile group based on the type of illness.
Article
Pediatrics
Marcello Della Corte, Elisa Cerchia, Marco Oderda, Paola Quarello, Franca Fagioli, Paolo Gontero, Simona Gerocarni Nappo
Summary: This case report presents a prechemotherapy transperitoneal robot-assisted partial nephrectomy (RAPN) for a unilateral, non-syndromic Wilms tumor in a child. The patient showed no tumor recurrence during a 28-month follow-up period.
Letter
Biotechnology & Applied Microbiology
Ilaria Galliano, Carla Alliaudi, Valentina Dapra, Paola Monta Nari, Cristina Calvi, Massimiliano Bergallo
MINERVA BIOTECHNOLOGY AND BIOMOLECULAR RESEARCH
(2022)