期刊
BIOSENSORS & BIOELECTRONICS
卷 195, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2021.113655
关键词
Internet of things (IoT); Loop-mediated isothermal amplification (LAMP); SARS-CoV-2; Smartphone; Molecular diagnostic
类别
资金
- Engineering Research Center of Excellence Program of Korea Ministry of Science, ICT & Future Planning (MSIP) /National Research Foundation of Korea (NRF) [2021R1A5A6002853]
- Korea Health Technology R&D Project/Korea Health Industry Development Institute (KHIDI) , Ministry of Health & Welfare of South Korea [HI20C0644]
The urgent need for rapid and accurate testing tools for SARS-CoV-2 detection can be met by IoT-based diagnostic devices, which can provide real-time monitoring with high sensitivity and accuracy.
Rapid and accurate testing tools for SARS-CoV-2 detection are urgently needed to prevent the spreading of the virus and to take timely governmental actions. Internet of things (IoT)-based diagnostic devices would be an ideal platform for point-of-care (POC) screening of COVID-19 and ubiquitous healthcare monitoring for patients. Herein, we present an advanced IoT-based POC device for real-time direct reverse-transcription-loop mediated isothermal amplification assay to detect SARS-CoV-2. The diagnostic system is miniaturized (10 cm [height] x 9 cm [width] x 5.5 cm [length]) and lightweight (320 g), which can be operated with a portable battery and a smartphone. Once a liquid sample was loaded into an integrated microfluidic chip, a series of sample lysis, nucleic amplification, and real-time monitoring of the fluorescent signals of amplicons were automatically performed. Four reaction chambers were patterned on the chip, targeting As1e, N, E genes and a negative control, so multiple genes of SARS-CoV-2 could be simultaneously analyzed. The fluorescence intensities in each chamber were measured by a CMOS camera upon excitation with a 488 nm LED light source. The recorded data were processed by a microprocessor inside the IoT-based POC device and transferred and displayed on the wirelessly connected smartphone in real-time. The positive results could be obtained using three primer sets of SARS-CoV-2 with a limit of detection of 2 x 10(1) genome copies/mu L, and the clinical sample of SARS-CoV-2 was successfully analyzed with high sensitivity and accuracy. Our platform could provide an advanced molecular diagnostic tool to test SARS-CoV-2 anytime and anywhere.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据