Article
Oncology
Yuanrui Liu, Rong Zhao, Zaichun Xie, Zhiyu Pang, Shengjie Chen, Qian Xu, Zhanfeng Zhang
Summary: In this study, circulating tumor cells (CTCs) were detected in the peripheral blood of tumor patients, and their numbers and subtypes were found to be related to different tumor diseases and stages. CTCs with mesenchymal phenotypes were closely associated with lymph node or vascular metastasis. CTC detection can aid in early diagnosis of tumor diseases and monitoring of disease progression.
Article
Chemistry, Analytical
Li Qin, Xiguang Han, Qiumei Feng, Po Wang
Summary: An innovative enzyme-free electrochemical sensor was designed using broom-like Ag@N,O-C hierarchical nanomaterials and entropy-driven DNA walker to analyze PIK3CA E545K ctDNA. The sensor demonstrated good selectivity, reproducibility, and practical application ability, making it a promising tool for disease diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Oncology
Retno Ningsi, Maha Elazezy, Luisa Stegat, Elena Laakmann, Sven Peine, Sabine Riethdorf, Volkmar Mueller, Klaus Pantel, Simon A. Joosse
Summary: CTCs have been found to be associated with metastasis and survival in breast cancer patients. Evaluating the expression of estrogen receptors (ER) in CTCs may help with personalized treatment. Some patients have both ER-positive and ER-negative CTCs at the time of metastasis, which may lead to resistance to hormonal therapy. Therefore, monitoring the status of CTC-ER in breast cancer patients can provide prognostic and predictive value.
Article
Biophysics
Syed Harris Hussain, Cesar S. Huertas, Arnan Mitchell, Anne-Laure Deman, Emmanuelle Laurenceau
Summary: Analysis of circulating tumor cells (CTCs) and their associated biomarkers can provide valuable information for lung and prostate cancer, aiding in early detection and personalized treatment selection. Biosensors offer an elegant alternative to conventional methods for real-time, multiplexed analysis of cancer biomarkers at lower concentrations.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Ke Wang, Zhijia Peng, Xiaogang Lin, Weiqi Nian, Xiaodong Zheng, Jayne Wu
Summary: This article discusses the application of electrochemical biosensors in ctDNA detection. They offer advantages such as high sensitivity, high specificity, low cost, and good portability. The article summarizes the choices and performance of different types of ctDNA sensors, and analyzes their development in the areas of Internet of Things, point-of-care testing, big data, and big health.
Article
Oncology
Ying Xu, Xue Ren, Tong Jiang, Shuang Lv, Kuanke Gao, Yunen Liu, Ying Yan
Summary: This study showed a high rate of positive CTC detection in patients with lung cancer, and the number, subtype, and hTERT-positive expression of CTCs were closely related to patients' overall response rate, progression-free survival, and overall survival with radiotherapy. EMCTCs and hTERT-positive expression of CTCs are expected to be important biological indicators for predicting radiotherapy efficacy and the prognosis in patients with lung cancer. These results may be useful in improving disease stratification for future clinical trials and may help in clinical decision-making.
Review
Chemistry, Analytical
Mahesh Padmalaya Bhat, Venkatachalam Thendral, Uluvangada Thammaiah Uthappa, Kyeong-Hwan Lee, Madhuprasad Kigga, Tariq Altalhi, Mahaveer D. Kurkuri, Krishna Kant
Summary: CTCs, circulating tumor cells, are widely used in clinical trials for tumor diagnosis, and capturing and isolating CTCs from whole blood samples has significant benefits in cancer diagnosis and treatment. Traditional methods are time-consuming and face challenges, while recent advances in microfluidic devices offer higher efficiency and accuracy in capturing and isolating CTCs.
Review
Chemistry, Analytical
Antonella Curulli
Summary: This review discusses the research advancements in food allergen detection using electrochemical biosensors. It focuses on the different types of recognition elements, the role of nanomaterials, the use of electrochemical techniques, and specific approaches for surface modification of electrodes. Furthermore, it critically analyzes the analytical performances of the most recent electrochemical sensors for allergen detection, and discusses the advantages, limitations, and potential applications of electrochemical biosensors for allergens.
Review
Biochemistry & Molecular Biology
Jozsef Petrik, Donatella Verbanac, Marija Fabijanec, Andrea Hulina-Tomaskovic, Andrea Ceri, Anita Somborac-Bacura, Roberta Petlevski, Marija Grdic Rajkovic, Lada Rumora, Bozo Kruslin, Mario Stefanovic, Neven Ljubicic, Neven Barsic, Antonija Hanzek, Luka Bockor, Ivana Celap, Alma Demirovic, Karmela Barisic
Summary: This review provides an overview of the research conducted on circulating tumor cells (CTCs) with the aim of finding suitable detection systems and assessing the possibility of clinical applications in patients with colorectal cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Chemistry, Physical
Man Wu, Yuhang Huang, Yang Zhou, Hui Zhao, Yuwei Lan, Zhibin Yu, Chunping Jia, Hui Cong, Jianlong Zhao
Summary: Circulating tumor cells (CTCs) enumeration is widely used for early diagnoses, chemotherapy efficacy evaluation, and cancer prognosis. New types of circulating cancer-related cells have been reported to be correlated to cancer diagnosis, metastasis, or prognosis, and they present challenges to microfluidic devices for CTCs detection. This review focuses on the properties and clinical significance of these novel circulating cancer-related cells, and discusses the challenges they pose to microfluidic chip enrichment.
Article
Biotechnology & Applied Microbiology
Rui Zhang, Qiannan You, Mingming Cheng, Mingfeng Ge, Qian Mei, Li Yang, Wen-Fei Dong, Zhimin Chang
Summary: CTCs, shed from solid primary tumors into the blood, increase the risk of metastasis and recurrence. The developed ultra-sensitive electrochemical cytosensor can capture, detect, and release EpCAM-positive tumor cells with good stability and detection accuracy.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Electrochemistry
Xiao-Rui Li, Yi-Ge Zhou
Summary: Circulating tumor cell (CTC) serves as a crucial biomarker for early cancer diagnosis, and electrochemical CTC sensors have shown advantages in simplicity, speed, cost-effectiveness, and miniaturization. Recent developments in electrochemical cytosensors over the past 5 years have focused on different detection principles with a particular emphasis on the challenges and importance of single CTC detection. Future prospects lie in impact methods for single CTC analysis.
ELECTROCHEMISTRY COMMUNICATIONS
(2021)
Article
Biophysics
Nong Yu, Guifeng Ma, Yuyao Chen, Shan Huang, Yalei Gong, Shuangshuang Li, Haiqin Gu, Honglan You, Peng Miao
Summary: We have developed a novel electrochemical biosensor for the detection of circulating tumor cells (CTCs) in peripheral blood. The biosensor uses manganese dioxide nanosheets (MnO2 NSs) and gold nanoparticles (AuNPs). The biosensor achieves a wide linear range and low limit of detection, and has great potential for rare cell analysis and investigation of cellular interactions.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Review
Chemistry, Analytical
Isaac Goldstein, Sobia Alyas, Waseem Asghar, Azhar Ilyas
Summary: Circulating tumor cells (CTCs), shed from tumors and circulating in the bloodstream, play a crucial role in cancer metastasis. Analyzing CTCs through liquid biopsy can significantly enhance researchers' understanding of cancer biology. However, the sparsity of CTCs poses a challenge in their detection and capture. This paper discusses and compares various biosensing techniques for isolating, detecting, and releasing/detaching CTCs, aiming to evaluate their efficacy, specificity, and cost in point-of-care (POC) settings.
Article
Plant Sciences
Zu-Jun Que, Yun Yang, Hai-Tao Liu, Wen-Ji Shang-Guan, Pan Yu, Li-Hua Zhu, He-Gen Li, Huai-Min Liu, Jian-Hui Tian
Summary: The study aimed to investigate the anti-metastatic mechanism of Jinfukang in lung cancer by intervening in circulating tumor cells (CTCs). Results showed that Jinfukang inhibited the proliferation, migration, and invasion of CTCs, as well as inducing anoikis through suppression of the integrin/Src pathway. This suggests that Jinfukang may exert its anti-metastatic effects in lung cancer through anoikis.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Fan Wu, Tingting Xu, Guangyao Zhao, Shuangshuang Meng, Mimi Wan, Bo Chi, Chun Mao, Jian Shen
Article
Chemistry, Analytical
Fang Peng, Tingting Xu, Fan Wu, Chunxue Ma, Yuhong Liu, Jianying Li, Bo Zhao, Chun Mao
Article
Chemistry, Multidisciplinary
Tingting Xu, Meilin Chu, Yinben Wu, Jiahuan Liu, Bo Chi, Hong Xu, Mimi Wan, Chun Mao
NEW JOURNAL OF CHEMISTRY
(2018)
Article
Polymer Science
Tingting Xu, Shuyue Zhan, Meihui Yi, Bo Chi, Hong Xu, Chun Mao
POLYMERS FOR ADVANCED TECHNOLOGIES
(2018)
Article
Chemistry, Multidisciplinary
Mi Mi Wan, Ting Ting Xu, Bo Chi, Meng Wang, Yangyang Huang, Qi Wang, Ting Li, Wen Qiang Yan, Huan Chen, Ping Xu, Chun Mao, Bo Zhao, Jian Shen, Hong Xu, Dong Quan Shi
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2019)
Article
Biophysics
Tingting Xu, Jiming Li, Shaoyin Zhang, Yongcan Jin, Rui Wang
BIOSENSORS & BIOELECTRONICS
(2019)
Article
Chemistry, Analytical
Tingting Xu, Hongqi Dai, Yongcan Jin
Article
Chemistry, Analytical
Huanli Gao, Tingting Xu, Jiahuan Zhou, Orlando J. Rojas, Ming He, Xingxiang Ji, Hongqi Dai
Summary: This article introduces a system for the rapid and ultra-sensitive detection of the prevalent foodborne pathogen Staphylococcus aureus. By utilizing a dual-aptamer-based sandwich immunobiosensor, the system demonstrates specificity and anti-fouling capacity, with signal amplification through highly conductive gold nanoparticles. Experimental results show that the immunobiosensor has an extended detection range and low detection limit, while maintaining stability for two weeks.
Article
Polymer Science
Tingting Xu, Huanli Gao, Orlando J. J. Rojas, Hongqi Dai
Summary: In this study, a conductive hydrogel was synthesized using carboxymethyl chitosan and sodium carboxymethyl cellulose as biopolymers, and coupled with conductive polypyrrole through hydrogen bonding. The addition of sodium lignosulfonate facilitated the efficient adsorption and reduction of silver ions, resulting in the embedding of silver nanoparticles in the hydrogel network and improving the electro-catalytic efficiency of the system. The prepared silver nanoparticle-embedded conductive hydrogel electrode showed excellent electro-catalytic activity towards hydroquinone (HQ).
Article
Chemistry, Multidisciplinary
Wenjin Wu, Yuping Yang, Lan Wang, Tingting Xu, Rui Wang
Summary: The mussel-inspired material EPD, designed and fabricated with a Schiff base structure, showed excellent detection limit and linear range as well as on-demand antibacterial activity for the simultaneous detection and elimination of S. aureus in food or drinks matrices. The immunosensor exhibited good accuracy in real milk samples, with values closely matching standard results obtained from the plate count method, and demonstrated good selectivity, reproducibility, and stability, highlighting its potential significant applications in drinks safety monitoring.
Article
Materials Science, Biomaterials
Zhiyong Liu, Tingting Xu, Meng Wang, Chun Mao, Bo Chi
JOURNAL OF MATERIALS CHEMISTRY B
(2020)
Article
Biochemistry & Molecular Biology
Priyanga Kumar, Noel Nesakumar, Jayashree Gopal, Sakthivel Sivasubramanian, Srinivasan Vedantham, John Bosco Balaguru Rayappan
Summary: This study developed an electrochemical biosensor for measuring MG levels in the plasma of type-2 diabetes mellitus patients. The biosensor showed a wide linear concentration range, high sensitivity, and a 90% correlation with ELISA data. It also exhibited a significant correlation with HbA1c and fasting plasma glucose levels.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Cristina Tortolini, Valeria Gigli, Antonio Angeloni, Federico Tasca, Nguyen T. K. Thanh, Riccarda Antiochia
Summary: The development of a novel voltammetric immunosensor for the detection of salivary MMP-8 at the point-of-care is described. The sensor showed good performance and comparable results to the conventional ELISA method when tested in real saliva samples. This biosensor is single-use, cost-effective, and requires a small quantity of test medium and a short preparation time.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Rimsha B. Jamal, Ulrich Bay Gosewinkel, Elena E. Ferapontova
Summary: Pathogen-triggered infections are a severe global threat to human health. Researchers have developed a fast and inexpensive electrocatalytic aptamer assay for the specific and ultrasensitive detection of E. coli, allowing for timely treatment and prevention. The method is fast, sensitive, and can be used in field and point-of-care applications for analysis of bacteria in the human environment.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Miao Liu, Yuwei Wang, Shanshan Tang, Wei Wang, Axin Liang, Aiqin Luo
Summary: An unlabeled ratiometric molecular imprinted electrochemiluminescence sensor was developed for the determination of trace uric acid using MXene@NaAsc, CdSe@ZnS quantum dots, and molecularly imprinted polymer composites modified glass carbon electrode. This sensor, with easy preparation, great selectivity, and excellent sensitivity, successfully detected uric acid in human serum.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Charlotte Da Silva, Camille Lamarche, Carole Pichereaux, Emmanuelle Mouton-Barbosa, Gauthier Demol, Sebastien Boisne, Etienne Dague, Odile Burlet-Schiltz, Flavien Pillet, Marie-Pierre Rols
Summary: Low-energy electron beams (LEEB) are a safe and practical sterilization solution for industrial applications. To address the limitations of LEEB, we developed a low-energy pulsed electron beam generator (LEPEB) that can effectively and efficiently eradicate bacteria in a wide range of industrial applications.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Daniel Dobrovodsky, Ales Danhel, Daniel Renciuk, Jean-Louis Mergny, Miroslav Fojta
Summary: In this study, N-methyl mesoporphyrin IX (NMM) was utilized as a voltammetric probe for the electrochemical detection of G4s. The detection of NMM was achieved by cyclic voltammetry on a hanging mercury drop electrode (HMDE) with a limit of detection (LOD) of 40 nM. The reduction signal of NMM was found to be significantly higher when G4 oligodeoxynucleotides (G4 ODNs) were present compared to single- or double-stranded ODNs or unfolded ODNs capable of forming G4s. Gradual transition from unstructured single strand to G4, induced by increasing concentrations of the G4 stabilizing K+ ions, was detected for the first time using electrochemical methods. Circular dichroism spectroscopy provided support for the obtained results. This work expands on the utilization of electrochemical probes for DNA secondary structure recognition and offers a proof of principle for the development of novel electroanalytical methods for nucleic acid structure studies.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Mengjiao Zhu, Yu Liu, Meiyue Wang, Tao Liu, Zhenyu Chu, Wanqin Jin
Summary: Early rapid diagnosis of COVID-19 is crucial for reducing the risk of severe symptoms and loss of lung function. Researchers have proposed an ultrafast and ultrasensitive DNA sensor that can accurately detect the virus in a short period of time, with outstanding selectivity.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Fatima-Zahra Ait-Itto, James A. Behan, Mathieu Martinez, Frederic Barriere
Summary: This study investigated the feasibility of using ancient marine sediments as inoculum for bioanode development in microbial fuel cells (MFC). The results showed the presence of two exoelectrogenic bacterial genera in these iron-rich sediments and confirmed that the development of the bioanode derived from the native microbiota. This study has important implications for understanding the role of these bacteria in broader paleoenvironmental phenomena.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Issa Fall, Bastien Doumeche, Sofiene Abdellaoui, Caroline Remond, Harivony Rakotoarivonina, Marjorie Ochs
Summary: This article presents a novel electrochemical tool based on lignin-coated paper electrodes for the detection and characterization of ligninolytic activity. The suitability of this method has been demonstrated using a catalaseperoxidase isolated from Thermobacillus xylanilyticus.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Chenyi Zhuo, Dehong Yu, Jiuying Cui, Zichun Song, Qianli Tang, Xianjiu Liao, Zhao Liu, Ning Xin, Lu Lou, Fenglei Gao
Summary: A label-free detection strategy for quantification of Apolipoprotein A4 (Apo-A4) has been developed based on rolling circle amplification (RCA) and proximity hybridization-induced DNAzyme-driven bipedal DNA walker. This method shows high sensitivity and selectivity for a wide range of protein targets.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Niloufar Fattahi, Jeffrey Reed, Evan Heronemus, Priyasha Fernando, Ryan Hansen, Prathap Parameswaran
Summary: In this study, polyethylene glycol hydrogels were developed as protective coatings for electroactive biofilms, improving their viability under low resource conditions and ammonia-N shocks.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Xiaopeng Hou, Qiongyi Cheng, Hui Wang
Summary: A self-cleaning paper-based microfluidic biosensor was developed using hydrophobic paper decorated with polymeric octadecyl trichlorosilane. The biosensor showed improved performance and could effectively measure the concentration of copper ions in livestock feed and manure.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Yanling Zhang, Jie Wang, Min Liu, Yun Ni, Yao Yue, Dawei He, Ruijiang Liu
Summary: An innovative electrochemical biosensor has been developed for sensitive detection of HER2 protein, an important tumor marker for breast cancer diagnosis and treatment evaluation. The biosensor utilizes specific recognition probes and nanomaterials, along with a unique sensing strategy, to achieve ultra-low detection limit and demonstrate excellent selectivity, reproducibility, and stability.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Humayun Amir, Vasanth Subramanian, Sundaram Sornambikai, Nagamony Ponpandian, Chinnusamy Viswanathan
Summary: In this study, the electrochemical detection of breast cancer biomarker HER2 was successfully achieved using the N-CQDs/GS as the detector. The BSA-modified HER2 Antibody/N-CQDs/GS immunoelectrode preserved excellent activity for the biosensor, while the GS electrode provided high stability and conductivity. The method exhibited a low detection limit and optimal specificity, stability, and reproducibility for detecting HER-2 protein in untreated blood samples from breast cancer patients.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Ilona Uzieliene, Anton Popov, Raminta Vaiciuleviciute, Gailute Kirdaite, Eiva Bernotiene, Almira Ramanaviciene
Summary: This review discusses the application of polypyrrole (Ppy), an electroconductive polymer, in cell culture experiments with electrical stimulation (ES) and its potential for tissue regeneration. Ppy can direct ES to cells and stimulate differentiation towards different cell lineages. Different types of Ppy materials and their combination with active molecules are explored.
BIOELECTROCHEMISTRY
(2024)
