Article
Multidisciplinary Sciences
Pola J. Verschoor, Fiona H. Greig, Justin J. Rochford, Giovanni Levate, Mirela Delibegovic, Dawn Thompson, Alasdair Leeson-Payne, Ruta Dekeryte, Ruth Banks, Joe W. Ramos, Graeme F. Nixon
Summary: PEA-15 plays a novel role in regulating the expansion of adipose tissue, with its deficiency leading to increased body weight and adipocyte cell size but also improving whole body insulin sensitivity, decreasing hepatic weight, and lowering serum triglycerides, showing a protective phenotype.
SCIENTIFIC REPORTS
(2021)
Article
Ophthalmology
Kwanghyun Lee, Jin-Ok Choi, Ahreum Hwang, Hyoung Won Bae, Chan Yun Kim
Summary: This study investigated the protective effects of astrocyte-derived CNTF on retinal ganglion cells (RGCs) and identified the signaling pathways involved. The results showed that astrocytes improved RGC viability and /33-tubulin expression, and the concentration of CNTF increased in the RGC-astrocyte co-culture medium. The protective effects of astrocytes were dependent on CNTF, suggesting its importance in mediating these effects. CNTF treatment alone also enhanced RGC viability and /33-tubulin expression, and increased the population of viable RGCs under oxidative stress. Different pathways, such as PI3K/AKT, JAK/STAT, and MAPK/ERK, were found to be associated with RGC viability and /33-tubulin expression. RNA sequencing revealed the genes upregulated and downregulated by CNTF treatment, providing insights into the molecular mechanisms involved in the protective effects. In conclusion, this study highlights the importance of astrocyte-derived CNTF in protecting RGCs and identifies novel genes and pathways associated with this protective effect. It may contribute to the development of treatments for RGC injury.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Article
Chemistry, Medicinal
Zhaowan Zheng, Zhenbang Xiao, Yuan-Lin He, Yanfei Tang, Lefan Li, Chunxia Zhou, Pengzhi Hong, Hui Luo, Zhong-Ji Qian
Summary: The study demonstrated that DAPTMGY from Isochrysis zhanjiangensis can scavenge ROS, increase endogenous antioxidants, and exert anti-photoaging effects through multiple pathways, providing protective effects against UVB-induced damage.
Article
Cell Biology
Emmanuelle S. Jecrois, Wang Zheng, Miriam Bornhorst, Yinghua Li, Daniel M. Treisman, Daphine Muguyo, Sharon Huynh, Shayne F. Andrew, Yuan Wang, Jingwen Jiang, Brianna R. Pierce, Hongmei Mao, Matthew K. Krause, Austin Friend, Francisco Nadal-Nicolas, Steven F. Stasheff, Wei Li, Hui Zong, Roger J. Packer, Yuan Zhu
Summary: NF1-OPGs arise from vulnerability to Mek-Erk/MAPK signaling dependency during gliogenesis, caused by disruption of the balance between stem-cell maintenance and gliogenesis in brain-derived migrating glial progenitors. Removal of specific genes or transient treatment with a low-dose MEK inhibitor can prevent NF1-OPG formation and neuronal degeneration.
DEVELOPMENTAL CELL
(2021)
Article
Multidisciplinary Sciences
Joyce Ikedife, Jianlin He, Yufeng Wei
Summary: Phosphorylation of PEA-15 mediates conformational changes and alters specific binding, regulating mitogen-activated protein kinase and apoptosis pathways.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Medicinal
Xiaochao Huang, Yuanhang Chen, Wentian Zhong, Zhikun Liu, Haijiang Zhang, Bin Zhang, Hengshan Wang
Summary: In this study, sixteen novel conjugates containing tubulin inhibitor and matrix metalloproteinase inhibitor were synthesized and evaluated for their activity. Compound 9e showed the most potent activity against various human cancer cells, including multidrug-resistant tumor cells, and exhibited lower cytotoxicity towards normal liver cells. It strongly inhibited tubulin polymerization, induced cell apoptosis and cell cycle arrest, and displayed inhibition of cell migration and moderate activity towards MMP-2, MMP-3, and MMP-9. Moreover, it induced apoptosis via the mitochondria-dependent pathway and caused ER stress. In vivo, 9e showed significant antitumor efficacy without systemic toxicity. Overall, compound 9e is a novel and promising agent for cancer therapy, acting as a dual MMPs and tubulin inhibitor.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Oncology
Yifeng Wu, Xianpeng Li, Mingliang Chen, Zhikun Liu, Xuanyu Zhang, Shusen Zheng, Xiao Xu
Summary: In this study, elevated levels of PED/PEA-15(S116) and P-p27(T187) were found in HCC tissues compared to adjacent and normal tissues, and their expression was closely associated with clinicopathological features and prognosis of patients with HCC. These proteins may serve as potential therapeutic targets for HCC.
Article
Critical Care Medicine
Liang Wu, Ning-ning Ji, Hang Wang, Jing-yu Hua, Guo-lin Sun, Pan-pan Chen, Rong Hua, Yong-mei Zhang
Summary: The study revealed that in a rat model of traumatic brain injury, IL-15 could induce neuronal apoptosis by enhancing CD8 T-cell function.
JOURNAL OF NEUROTRAUMA
(2021)
Article
Oncology
Ariege Bizanti, Priyanka Chandrashekar, Robert Steward
Summary: Biomechanical behavior of astrocytes is influenced by substrate stiffness, with increasing stiffness leading to higher intercellular stresses, strain energies, and tractions, while cell velocities decrease. This study sheds light on the importance of substrate stiffness on astrocytic function and could have implications for brain pathology and physiology research.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Cell & Tissue Engineering
Qin Mei, Lingjun Ye, Hao Lin, Chunyou Chen
Summary: The research revealed that miR-133b plays a regulatory role in astrocyte proliferation and apoptosis by targeting down-regulated expression of Kif15 protein. Overexpression of miR-133b led to decreased cellular proliferation and increased apoptosis rates, possibly through down-regulating Kif15 expression.
JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING
(2021)
Article
Chemistry, Physical
Sarbani Mishra, Mansi Sharma, Mahendra Kumar Singh, Sanghamitra Pati, Budheswar Dehury
Summary: The study explores the potential connection between LRRC15 and SARS-CoV-2 and investigates the structure-function mechanism and interaction mode. The findings suggest alternative binding modes between the RBD and LRR units of LRRC15, indicating their differential roles. However, further experimental validations are required to unravel the unknown mechanism and distinctive features of this host receptor in COVID-19.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Biochemistry & Molecular Biology
Omri Zveik, Ariel Rechtman, Nitzan Haham, Irit Adini, Tamar Canello, Iris Lavon, Livnat Brill, Adi Vaknin-Dembinsky
Summary: This study found that sera from patients with neuromyelitis optica (NMO) may cause DNA damage and apoptosis in astrocytes, suggesting a new mechanism in the pathogenesis of NMO and potential targets for therapeutic intervention.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Food Science & Technology
Damijana Mojca Juric, Klara Bulc Rozman, Metoda Lipnik-Stangelj, Dusan Suput, Miran Brvar
Summary: The influence of cannabidiol (CBD) on brain development is not well understood, but it may interfere with the endogenous cannabinoid system during critical periods, leading to apoptosis and necroptosis in neurons and astrocytes.
Article
Biochemistry & Molecular Biology
Xiaojie Xu, Yuejiao Zhang, Jing Zhang, Dongmei Wang, Hongxu Yang, Qian Liu, Peng Zhou, Yazhou Wang, Liu Yang, Meiqing Wang
Summary: This study found that the temporomandibular joint (TMJ) cartilage is sensitive to dental biomechanical stimulation, and the cells in different zones of the cartilage have different differentiation states. By using specific gene markers and cell ablation, researchers discovered the interdependence between cells in different zones of the cartilage, which is essential for maintaining the structure and function of the cartilage.
Article
Multidisciplinary Sciences
Maisel J. Caliva, Won Seok Yang, Shirley Young-Robbins, Ming Zhou, Hana Yoon, Michelle L. Matter, Mark L. Grimes, Thomas Conrads, Joe William Ramos
Summary: Endosomal trafficking of integrins modulates cell motility, with PEA-15 enriching in endosomes, promoting endosomal internalization of α5β1 integrin and cellular migration, where the promotion of migration by PEA-15 is dependent on phosphorylation at specific serine sites.
SCIENTIFIC REPORTS
(2021)
Article
Ophthalmology
Samih Alqawlaq, John G. Flanagan, Jeremy M. Sivak
EXPERIMENTAL EYE RESEARCH
(2019)
Article
Ophthalmology
Faryan Tayyari, Lee-Anne Khuu, Jeremy M. Sivak, John G. Flanagan, Shaun Singer, Michael H. Brent, Christopher Hudson
ACTA OPHTHALMOLOGICA
(2019)
Review
Food Science & Technology
Changmo Kim, Izhar Livne-Bar, Karsten Gronert, Jeremy M. Sivak
MOLECULAR NUTRITION & FOOD RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Samih Alqawlaq, Izhar Livne-Bar, Declan Williams, Joseph D'Ercole, Sara W. Leung, Darren Chan, Alessandra Tuccitto, Alessandro Datti, Jeffrey L. Wrana, Anita H. Corbett, Gerold Schmitt-Ulms, Jeremy M. Sivak
Summary: Astrocytes secrete signals that support neuronal survival by protecting against neurotoxicity, oxidative stress, and apoptotic cascades, with PI3K identified as a central player in this neuroprotective process. Proteomic analysis revealed PI3K interactors, including PDGFRA, ZC3H14, and THOC1, with ZC3H14 playing a crucial role in promoting PDGF-induced neuroprotection and presenting a potential strategy for astrocyte-secreted prosurvival signals.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
David J. Mathew, Izhar Livne-Bar, Jeremy M. Sivak
Summary: A new glaucoma model has been introduced in this study, resulting in a gradual and sustained increase in intraocular pressure, which more closely resembles clinical glaucoma. The model demonstrated relevant changes in retinal and optic nerve pathology associated with ocular hypertension, without inducing eye angle inflammation.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Multidisciplinary
C. Frank Lee, Carla E. Brown, Alexander J. Nielsen, Changmo Kim, Izhar Livne-Bar, Philip J. Parsons, Christophe Boldron, Francois Autelitano, Donald F. Weaver, Jeremy M. Sivak, Mark A. Reed
Summary: Two stereocontrolled, efficient, and modular syntheses of eicosanoid lipoxin B4 have been reported. These methods provide high yields and synthetic flexibility, enabling the synthesis of large quantities of lipoxin B4, as well as new analogs and chemical probes.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Article
Cell Biology
Nevena Vicic, Xiaoxin Guo, Darren Chan, John G. Flanagan, Ian A. Sigal, Jeremy M. Sivak
Summary: This study found that moderate biomechanical strain damages cell membranes, increases permeability, and induces the aggregation of A4 protein at injury sites. Loss of A4 protein increases permeability, while exogenous A4 protein reduces it. Furthermore, similar findings were observed in the optic nerve head of glaucoma patients. These studies provide a new model for understanding the pathogenesis of glaucoma.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Meeting Abstract
Ophthalmology
Jenny Wanyu Zhang, Darren Chan, Jeremy M. Sivak
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Meeting Abstract
Ophthalmology
David Jose Mathew, Izhar Livne-Bar, Darren Chan, Yvonne Buys, Graham E. Trope, Marisa Sit, Becca A. Flitter, John G. Flanagan, Karsten Gronert, Jeremy M. Sivak
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Meeting Abstract
Ophthalmology
Aminat Seala Adama, Karen G. Wigg, Alessandra Tuccitto, Jeremy M. Sivak
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Article
Multidisciplinary Sciences
D. Chan, G. J. Won, A. T. Read, C. R. Ethier, E. Thackaberry, S. R. Crowell, H. Booler, V Bantseev, J. M. Sivak
Summary: This study investigates the movement of particles in the eye during intravitreal drug delivery. Using an organotypic perfusion strategy and experiments in pig and human eyes, it is found that the aggregate particle size plays a crucial role in determining their movement within the eye.
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2022)
Meeting Abstract
Ophthalmology
David Mathew, Izhar Livne-Bar, Jeremy Sivak
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Meeting Abstract
Ophthalmology
David J. Mathew, Izhar Livne-bar, Jeremy M. Sivak
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2020)
Meeting Abstract
Ophthalmology
Jeremy M. Sivak, Samih Alqawlaq, Izhar Livne-Bar, Darren Chan, Gerold Schmitt-Ulms
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2019)
Meeting Abstract
Ophthalmology
Izhar Livne-Bar, John G. Flanagan, Karsten Gronert, Jeremy M. Sivak
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2019)