期刊
EXPERIMENTAL AND MOLECULAR PATHOLOGY
卷 101, 期 3, 页码 332-340出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.yexmp.2016.11.004
关键词
Vascular wall-resident stem/progenitor cells; Vascular smooth muscle cells; Proliferation; Neointimal formation
类别
资金
- National Natural Science Foundation of China [81170134]
Cell culture and carotid injury studies with SD rats were performed to investigate the roles of CD34(+) vascular wall-resident stem/progenitor cells (VRS/Pcs) and vascular smooth muscle cells (SMCs) in neointimal formation. In vitro, the media-isolated SM MHC+ SMCs occupied 93.92 +/- 8.62% of total BrdU(+) cells, whereas the CD34(+) cells, only 2.61 +/- 0.82%, indicating that the cell expansion in SMC culture was attributed to SM MHC+ SMCs. The adventitia-isolated CD34(+) VRS/Pcs responded to PDGF-BB by differentiating into SMC-like cells which expressed SM22 alpha (an early stage SMC marker), but seldom SM MHC (a late stage SMC marker). In carotid injury model, the CD34(+) VRS/Pcs differentiated SMC-like cells migrated in very few numbers into only the outer layer of the media, and this was further confirmed by a cell tracking analysis. While the neointimal cells were consistently SM MHC+ and CD34(-) SMCs during whole course of the post-injury remodeling. Thus it is speculated that the adventitial CD34(+) VRS/Pcs, at least in rat model, do not directly participate in neointimal formation, but function to maintain homeostasis of the media during injury-induced vascular wall remodeling. (C) 2016 Elsevier Inc. All rights reserved.
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