期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 60, 期 36, 页码 19759-19765出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202103599
关键词
caged compounds; cell imaging; lipid transport; phosphoinositides; photo-crosslinking
资金
- NIH [R01GM127631, Transregio 186]
- German Research Foundation (DFG)
Researchers synthesized novel phosphoinositide polyphosphate derivatives with multifunctionality and analyzed their intracellular transport using click chemistry. They found that certain derivatives can rapidly traffic to the plasma membrane, and confirmed the involvement of lipid binding proteins ATP11A and MPP6 in the transport of PI(3,4,5)P-3.
We synthesized the first multifunctionalized phosphoinositide polyphosphate derivatives featuring a photo-removable protecting group (cage), a photo-crosslinkable diazirine group, and a terminal alkyne group useful for click chemistry. We demonstrate that the lipid derivatives readily enter cells. After photo-crosslinking, cell fixation and fluorescent tagging via click chemistry, we determined the intracellular location of the lipid derivatives before and after uncaging of the lipids. We find that there is rapid trafficking of PI(3,4)P-2 and PI(3,4,5)P-3 derivatives to the plasma membrane, opening the intriguing possibility that there is active transport of these lipids involved. We employed the photo-crosslinking and click chemistry functions to analyze the proteome of PI(3,4,5)P-3-binding proteins. From the latter, we validated by RNAi that the putative lipid binding proteins ATP11A and MPP6 are involved in the transport of PI(3,4,5)P-3 to the plasma membrane.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据