Article
Chemistry, Analytical
Mengxi Chen, Ruilin Duan, Shijun Xu, Zhijuan Duan, Quan Yuan, Fan Xia, Fujian Huang
Summary: A photoactivated DNA walker signal amplification strategy was developed for photocontrollable imaging of cancer-related miRNA in single living cells. The method allows on-demand activation of the DNA walker for specified signal amplification imaging of cancer-related miRNA.
ANALYTICAL CHEMISTRY
(2021)
Article
Nanoscience & Nanotechnology
Hongki Kim, Chenlei Gu, Salman Ahmad Mustfa, Davide Alessandro Martella, Cong Wang, Yikai Wang, Ciro Chiappini
Summary: The CRISPR/Cas systems have been developed as powerful molecular biosensing tools based on their collateral cleavage activity. However, applying the collateral cleavage activity for in situ intracellular detection is challenging. In this study, a CRISPR/Cas-assisted nanoneedle sensor (nanoCRISPR) is introduced for intracellular ATP detection, which overcomes the challenges associated with intracellular collateral cleavage. The nanoCRISPR approach enables the detection of intracellular ATP in live cells within 30 minutes without impacting cell viability.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Si-Yuan Yu, Yi-Fan Ruan, Yi-Li Liu, De-Man Han, Hong Zhou, Wei-Wei Zhao, Dechen Jiang, Jing-Juan Xu, Hong-Yuan Chen
Summary: The study introduces a photocontrolled nanopipette capable of probing cellular ATP gradient at single-cell level with high sensitivity, selectivity, and reversibility, utilizing ATP-specific azobenzene-incorporated DNA aptamer strands for sensible transduction of variable nanopore size by ionic currents. The photoisomerized conformational change ensures noninvasive regeneration and repeated detection, allowing inducement and inhibition of cellular ATP to be probed.
Review
Materials Science, Multidisciplinary
Xianyang Zhang, Pengfei Chen, Siwuxie He, Bowen Jiang, Yong Wang, Yonghua Cheng, Jian Peng, Francis Verpoort, John Wang, Zongkui Kou
Summary: Biosensors featuring single molecule detection offer great opportunities in various fields, but face challenges due to the lack of activity, precision molecule selectivity, and understanding of the operating mechanism. Single-atom catalysts (SACs), particularly those that mimic the natural metalloenzyme structure, provide practical-use feasibilities for single molecule detections with high molecular selectivity and easy fabrication. This review discusses the history, advantages, and applications of SACs in molecule-scale biosensors, emphasizing their sensing modes and coordination-modulated signal amplifications.
Article
Microbiology
Ruining Li, Zhiwei Li, Chenxi Huang, Yifeng Ding, Jia Wang, Xiaohong Wang
Summary: This study reported the potential of a Staphylococcus phage named LSA2302 for the rapid detection of S. aureus. The phage's biological characteristics were identified, and genomic analysis revealed its lack of pathogenicity or drug resistance genes. A biosensing method for S. aureus detection was established using phage-functionalized magnetic beads (pMB) integrated with ATP bioluminescence assays.
Article
Biochemical Research Methods
Vincent Bayle, Jean-Bernard Fiche, Claire Burny, Matthieu Pierre Platre, Marcelo Nollmann, Alexandre Martiniere, Yvon Jaillais
Summary: This article introduces the implementation and application of super-resolution single-particle tracking photoactivated localization microscopy (sptPALM) in live roots of Arabidopsis thaliana. The advantages and limitations of sptPALM for studying membrane protein dynamics are discussed, along with technical details for handling and imaging the samples, and practical guidance for data collection and analysis. The protocol enables plant researchers to perform sptPALM using a reproducible benchmarked protocol, which involves 3-4 hours of imaging followed by 3-4 days of image processing and data analysis.
Article
Nanoscience & Nanotechnology
Jilei Chen, Lu Zhang, Shulan Li, Feng-Lei Jiang, Peng Jiang, Yi Liu
Summary: A method for water-soluble high-quality Cu-deficient CISe QDs was presented, allowing flexible control of composition by adjusting reaction temperature, reaction time, and Cu/In precursor ratio. The study revealed the mechanism of copper content on the fluorescence of CISe QDs, showing enhanced fluorescence with increased copper vacancies but possibly decreased fluorescence due to excessive vacancies. Optimized CISe QDs with maximum quantum yield were obtained for the development of a highly sensitive turn-on fluorescent probe for ATP detection in living cells.
ACS APPLIED NANO MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Gang Song, Hao Heng, Jiaqi Wang, Ronghua Liu, Yiming Huang, Huan Lu, Ke Du, Fude Feng, Shu Wang
Summary: The study involves the photoactivated trimerization of 2,3,3-trimethyl-3H-indole derivatives to produce the near infrared fluorophore Cy5. This synthetic method is air-tolerant, photosensitizer free, metal free, and condensation agent free. Living cells are able to produce Cy5 within minutes under white light irradiation at a low power intensity, with the monomer as the only exogenous agent, showing promise for applications in cell studies.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Analytical
Jingjing Qian, Yixia Yang, Feng Gong, Xiaoyun Shan, Xinghu Ji, Zhike He
Summary: A novel method combining NanoCluster Beacon (NCB) and rolling cycle amplification (RCA) was developed for ATP detection. NCB was composed of silver clusters (P2-AgNCs), a converter (P3), and a linker sequence (P1) that kept them in close proximity. The fluorescence performance of NCB correlated with the status of the AgNCs, allowing controllable color switching from green to red. Under optimized conditions, the ratio of fluorescence intensities at 630 nm and 542 nm (F630 nm/F542 nm, F630/542) showed a linear correlation with the logarithmic ATP concentration, with a detection limit of 122 pM. The proposed method also demonstrated good performance in human serums, indicating its potential for clinical diagnosis.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Luying Ou, Rui Guo, Weiying Lin
Summary: A simple off-on H2S fluorescent probe CMHS based on coumarin has been designed with high selectivity and low detection limit, enabling successful visualization of exogenous and endogenous H2S in HeLa cells with low cytotoxicity.
ANALYTICAL METHODS
(2021)
Article
Chemistry, Multidisciplinary
Chenchen Xing, Jianlin Deng, Wenlong Fu, Jichao Li, Lijie Xu, Ruihao Sun, Dan Wang, Chengwen Li, Kang Liang, Meng Gao, Biao Kong
Summary: Mesoporous silica nanoparticles (MSNs) functionalized with benzimidazole-derived fluorescent molecules (DHBM) were utilized for highly sensitive and selective detection of Cu2+ through a feasible interfacial superassembly strategy, showing a quenching effect on Cu2+ in aqueous solutions with a detection limit as low as 7.69x10(-8) M. The solid-state sensor demonstrated good recyclability, and the silica framework enhanced the photostability of DHBM. These findings suggest that the DHBM-MSN fluorescent sensor has great potential for practical and biomedical applications.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Article
Chemistry, Analytical
Xinran Dong, Yuchen Huang, Dechen Jiang
Summary: A fluorescent polymerase chain reaction (PCR) nanokit was established for detecting specific DNA sequences in single living cells. This strategy involves sorting the DNA sequence in a cellular nucleus into a nanopipette, where it reacts with PCR kit components to complete a PCR amplification reaction. The presence of the target DNA sequence is validated through an increase in fluorescence, and the nanokit offers a general and highly sensitive approach for single cell analysis.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Zihao Zhang, Jinshu Tian, Yubing Lu, Shize Yang, Dong Jiang, Weixin Huang, Yixiao Li, Jiyun Hong, Adam S. Hoffman, Simon R. Bare, Mark H. Engelhard, Abhaya K. Datye, Yong Wang
Summary: The use of single atoms of platinum group metals on CeO2 is a promising approach to reduce precious metal requirements for automobile exhaust treatment catalysts. Here, the authors discovered that by manipulating the calcination temperatures, they could control the configuration of Pt1 on the CeO2 surface, leading to differences in CO oxidation activity.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Analytical
Leilei Yao, Weijie Zhang, Caixia Yin, Yongbin Zhang, Fangjun Huo
Summary: A dual-channel fluorescent probe RhB-NA was designed and synthesized for in situ imaging of ATP in living cells. This probe can detect fluctuations in ATP levels after mitochondrial state changes, which is of great significance for studying physiological diseases related to ATP.
Article
Biochemistry & Molecular Biology
Seyta Ley-Ngardigal, Giulia Bertolin
Summary: ATP, as the most universal and essential energy molecule in cells, plays a crucial role in various biological functions. Understanding its production and hydrolysis processes is necessary for comprehending its physiological and pathological functions. This review discusses the organization of electron transport chain and ATP synthase, methods for estimating ATP quantities in cells, and genetically encoded biosensors for monitoring ATP levels in living cells.
Editorial Material
Chemistry, Multidisciplinary
Yuchen Wu, Fan Xia, Shutao Wang
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
(2023)
Editorial Material
Chemistry, Multidisciplinary
Lei Xu, Linfeng Chen, Fan Xia
Summary: A recent study found that a superhydrophobic surface can significantly improve the pervaporation separation of ethanol-water mixture, which has broad implications for the design of new separation membranes.
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
(2023)
Article
Chemistry, Multidisciplinary
Guangwen Lu, Niya Lin, Zhaojun Chen, Wenlian Jiang, Jing-Jing Hu, Fan Xia, Xiaoding Lou
Summary: Cancers and chronic diseases are global health problems, and the detection of biomolecules using nanopores/nanochannels has faced limitations. However, the design of nanopores/nanochannels based on electrical and optical dual signal response has provided new opportunities for biological detection, allowing the identification of chemical compositions and conformations of biomolecules.
CHINESE JOURNAL OF CHEMISTRY
(2023)
Article
Nanoscience & Nanotechnology
Jun Dai, Simin Wei, Jiarong Xu, Huiying Xue, Zhaojun Chen, Meng Wu, Wei Chen, Xiaoding Lou, Fan Xia, Shixuan Wang
Summary: A microneedle-based device combined with aggregation-induced emission (AIE) photosensitizers (PSs) was constructed to achieve enhanced metronomic photodynamic therapy (mPDT) for cancer. The strong anti-photobleaching property of the AIE PS allows for superior photosensitivity even after prolonged light exposure. The delivery of the AIE PS to the tumor through the microneedle device provides better treatment outcomes and convenience.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Jing-Jing Hu, Wenlian Jiang, Yujuan Qiao, Qun Ma, Qiujiao Du, Jian-Hui Jiang, Xiaoding Lou, Fan Xia
Summary: Functional probes on the outer surface of nanochannel systems can be utilized for the detection of biotargets. By modulating the wettability on the outer surface, the detection sensitivity of matrix metalloproteinase-2 (MMP-2) can be improved. This study demonstrates that wettability regulation can be utilized as a variation factor to enhance the design strategy of a probe for biotarget detection.
Article
Chemistry, Multidisciplinary
Fujian Huang, Huiying Xue, Yuzhe Fu, Yu Ouyang, Danlong Chen, Fan Xia, Itamar Willner
Summary: Nucleic acid-functionalized liposomes modified with o-nitrobenzyl phosphate-caged hairpin units and pH-responsive C-G•C+ triplex forming strands are used for the light and pH-triggered fusion of three types of loaded liposomes. The fusion processes are monitored using light-scattering size enlargement measurements, optical methods, and biocatalytic cascades. The fused liposomes serve as functional protocells for the integration of biocatalytic machineries, such as the synthesis of a Mg2+-ion-dependent DNAzyme and the transcription of Malachite Green-RNA aptamer product by an autonomous polymerization/nickase machinery.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Liyun Zhang, Linfeng Chen, Lei Xu, Huan Zhao, Ruyi Wen, Fan Xia
Summary: Inspired by gastrointestinal peristalsis, researchers have designed and constructed double-layered tubular hydrogel actuators for the directional transport of highly viscous liquids. The actuators can transport liquids with viscosities ranging from approximately 1000 mPa s to >80000 mPa s under the control of an 808 nm laser. This work expands the research scope of liquid transportation and has potential applications in viscous-liquid-based microfluidics, artificial blood vessels, and soft robots.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Zhijuan Duan, Yu Ouyang, Yuzhe Fu, Fujian Huang, Fan Xia, Itamar Willner
Summary: In this study, optical and electrochemical probes were introduced for the quantitative assessment of cytochrome c (Cyt. c) in cellular compartments at the single cell level. These probes utilize photoresponsive o-nitrobenzylphosphate ester-caged Cyt. c aptamer constituents, which are uncaged by light stimuli, allowing the spatiotemporal detection of Cyt. c through the formation of Cyt. c/aptamer complexes under apoptotic or non-apoptotic conditions. These probes were applied to distinguish the contents of Cyt. c in cellular compartments of epithelial MCF-10A breast cells and malignant MCF-7 and MDA-MB-231 breast cells.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Changgang Li, Shuolei Deng, Wenhao Feng, Yaowen Cao, Jiaxuan Bai, Xiaocong Tian, Yifan Dong, Fan Xia
Summary: Hybrid solid-state electrolytes (HSSEs) with dendrite inhibition properties are successfully developed through universal room-temperature 3-dimensional printing (RT-3DP) strategy. The porous MOF filler in the HSSEs promotes homogeneity of lithium deposition and the formation of C-OCO3Li, ROLi, LiF mesophases, resulting in enhanced dendrite inhibition and improved Li+ migration in PEO chain.
Article
Chemistry, Analytical
Huizhen Li, Yunhui Li, Cenlin Gui, Daqi Chen, Lanfang Chen, Le Luo, Guobao Huang, Yang Yuan, Rong He, Fan Xia, Jiahai Wang
Summary: In this study, it has been confirmed that bare glass nanopores can accurately measure various lengths of DNA duplexes without adding organic solvent and embedding polymer hydrogel. The measurement has a resolution of 200 base pairs and shows well-separated peak amplitudes of blockage currents. By utilizing this capability, amplicons from PCR amplification can be discriminated without fluorescent labeling. This method can also discriminate various pathogenic bacteria and viruses, including SARS-CoV-2 and its mutants, in clinical samples with high resolution. Furthermore, the bare glass nanopore can quantitatively measure PCR amplicons without the need for extra labeling, with a detection range from 0.75 aM to 7.5 pM and a detection limit of 7.5 aM.
Article
Chemistry, Multidisciplinary
Yujuan Qiao, Jing-Jing Hu, Yuxin Hu, Chong Duan, Wenlian Jiang, Qun Ma, Yuning Hong, Wei Hua Huang, Fan Xia, Xiaoding Lou
Summary: We present a platform for detecting unfolded proteins using electrical and optical signals in a nanochannel array with outer-surface probes. The platform can differentiate folded and unfolded protein states based on the exposure of a single cysteine thiol group, allowing the distinction between normal cells and Huntington's disease mutant cells. This study provides an effective approach for analyzing proteins with distinct conformations and holds promise for facilitating the diagnoses of protein conformation-related diseases.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Chemistry, Multidisciplinary
Yu Huang, Lingxiao Liu, Cihui Luo, Wei Liu, Xiaoding Lou, Lei Jiang, Fan Xia
Summary: Bio-markers, such as ions, small molecules, nucleic acids, peptides, proteins, and cells, are important in living organisms and biological processes. Accurate detection of these bio-markers is significant for studying their functions, molecular diagnosis, and understanding life processes. Solid-state nanochannel-based sensing systems have shown promise in detecting bio-markers, especially with the use of outer-surface focused nanochannels. These nanochannels allow for in situ detection of large analytes and ultra-high sensitivity detection with a clear mechanism.
CHEMICAL SOCIETY REVIEWS
(2023)
Review
Materials Science, Multidisciplinary
Qun Ma, Liang Chen, Pengcheng Gao, Fan Xia
Article
Chemistry, Analytical
Shuwen Chen, Zhikang Cao, Kang Zhou, Shaoguang Li, Hui Li, Kaichen Xu, Haibin Tang, Heng Deng, Qitao Zhou, Jing Pan, Fan Xia
Summary: A wearable sweat sensor with excellent detection performance and mechanical stability has been designed and fabricated. It can simultaneously detect uric acid, tyrosine, and ascorbic acid with high sensitivity. The sensor maintains good uric acid sensing performance even after 20,000 bends. It has potential applications in health monitoring for both gout patients and healthy individuals.
Article
Chemistry, Multidisciplinary
Xiaojin Zhang, Yichuan Wang, Yu Dai, Fan Xia
Summary: We fabricated peptide-nanoparticle conjugates (AuNP@CDs-Azo-peptide) by the self-assembly of cyclodextrin-capped gold nanoparticles (AuNP@CDs) and azobenzene-terminated peptide (Azo-peptide) through host-guest interactions. AuNP@CDs-Azo-peptide exhibits hydrolase-like activity, which can be tuned by amino acid sequences.