期刊
AMERICAN JOURNAL OF SURGICAL PATHOLOGY
卷 45, 期 9, 页码 1274-1281出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/PAS.0000000000001760
关键词
SP142; 73-10; immunohistochemistry; metaplastic breast carcinoma; triple negative; programmed death-ligand 1
资金
- National Institutes of Health/National Cancer Institute Cancer Center Support Grant [P30CA008748]
- Breast Cancer Research Foundation
- Cycle for Survival grants
- Roche Tissue Diagnostics
Immunotherapy targeting PD-L1/programmed death-1 may benefit patients with MpBC, with most cases showing PD-L1 positivity in immune cells. However, there is variability in PD-L1 expression rates depending on the assay used. The SP142 assay demonstrates distinct expression patterns compared to E1L3n and 73-10, highlighting the importance of considering assay-specific characteristics in interpreting PD-L1 expression.
Immunotherapy for the treatment of programmed death-ligand 1 (PD-L1) positive locally advanced or metastatic triple negative breast cancer may benefit patients with metaplastic breast cancer (MpBC). Previous study of PD-L1 in MpBC scored tumor cells (TCs), different from Food and Drug Administration-approved scoring methods. We sought to define PD-L1 expression in MpBCs and to evaluate concordance of 3 PD-L1 assays. Primary, treatment naive MpBC treated at our Center from 1998 to 2019 were identified. PD-L1 expression was assessed using SP142, E1L3n, and 73-10. We evaluated PD-L1 expression on tumor infiltrating immune cells (IC) and also in TCs. For each assay, we scored PD-L1 expression using >= 1% IC expression according to the IMpassion130 trial criteria and using combined positive score (CPS) >= 10 according to the KEYNOTE-355 trial cutoff. A total of 42 MpBCs were identified. Most MpBC had PD-L1 positivity in >= 1% IC with all 3 assays (95%, 95%, 86%) in contrast to a maximum 71% with a CPS >= 10. PD-L1 IC expression was comparable between the SP142 and 73-10 assays and was lowest with E1L3n. PD-L1 TC expression was lowest using SP142. The overall concordance for IC scoring was 88% while 62% had concordant CPS. For each assay, the results of the 2 scoring algorithms were not interchangeable. The SP142 assay showed distinct expression patterns between IC (granular, dot-like) and TC (membranous) while 73-10 and E1L3n showed membranous and/or cytoplasmic expression in both IC and TC. Most MpBC in our cohort were positive for PD-L1 indicating eligibility for anti-PD-L1/programmed death-1 immunotherapy.
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