Article
Biochemistry & Molecular Biology
Irene Castellano-Pellicena, Ciaran G. Morrison, Mike Bell, Clare O'Connor, Desmond J. Tobin
Summary: Melanin fate within basal keratinocytes is regulated by asymmetric organelle movement during mitosis, with centrosomes and centriolar satellites facilitating the preferred apical distribution of melanin granules. This mechanism may control melanin's strategic position within UVR-exposed KCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Dermatology
Silvia Vidali, Rene G. Feichtinger, Michael Emberger, Susanne Maria Brunner, Stefanie Gaisbauer, Thomas Blatt, William J. Smiles, Christina Kreutzer, Julia M. Weise, Barbara Kofler
Summary: The decline of mitochondrial function is directly linked to skin ageing phenotypes. The protein levels of complex V and TFAM in the epidermis were significantly reduced in older subjects, independent of UV exposure. These alterations may impair mitochondrial functions and exacerbate cutaneous ageing.
EXPERIMENTAL DERMATOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Paula Montero, Maria Jose Villarroel, Ines Roger, Anselm Morell, Javier Milara, Julio Cortijo
Summary: Solar radiation can lead to various negative effects on the skin, such as sunburn, inflammation, and DNA damage. One potential approach to photoprotection is by targeting the transcription factor Nrf2, and obacunone has been identified as a potent Nrf2 agonist. This study aims to evaluate the relevance of full-thickness skin models in photoprotection studies and explore the photoprotective effects of obacunone in human keratinocytes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Geriatrics & Gerontology
Julia T. Oliveira, Vanja Dakic, Gabriela Vitoria, Carolina da S. G. Pedrosa, Mayara Mendes, Luiz Guilherme H. S. Aragao, Thyago R. Cardim-Pires, Damien Lelievre, Daniel Rodrigues Furtado, Roberta O. Pinheiro, Debora Foguel, Lionel Breton, Charbel Bouez, Rodrigo De Vecchi, Marilia Zaluar P. Guimaraes, Stevens Rehen
Summary: This study reveals that oligomeric alpha-Synuclein (O alpha-Syn) induces inflammation and degeneration in the skin, leading to a decrease in keratinocyte proliferation and thickness of the epidermis. These findings shed light on the potential role of O alpha-Syn in the aging process of the skin.
NEUROBIOLOGY OF AGING
(2022)
Article
Dermatology
Lydia Costello, Kirsty Goncalves, Paola De Los Santos Gomez, Amy Simpson, Victoria Maltman, Pamela Ritchie, Ryan Tasseff, Robert Isfort, Teresa Dicolandrea, Xingtao Wei, Arto Maatta, Iakowos Karakesisoglou, Ewa Markiewicz, Charles C. Bascom, Stefan Przyborski
Summary: Skin ageing is a complex process influenced by internal and external factors. This study analyzed skin parameters in individuals with Fitzpatrick skin types II-III to understand the effects of age and photoexposure. Differences in epidermal thickness were attributed to changes in the dermal-epidermal junction (DEJ). Basal keratinocytes showed increased cell size and a more columnar phenotype in photoexposed sites, suggesting accelerated photoageing. The study highlights the importance of early skin care education and photoprotection.
EXPERIMENTAL DERMATOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Federica Papaccio, Andrea D'Arino, Silvia Caputo, Barbara Bellei
Summary: Skin aging is characterized by fine lines, wrinkling, loss of elasticity and volume, and rough-textured appearance. The alteration of oxidative balance plays a key role in driving senescence, with reactive oxygen species (ROS) being produced through cellular oxidative metabolism and environmental factors causing loss of redox equilibrium. In addition to the structural changes, aging skin also loses its natural functional characteristics and regenerative potential. The skin immune system undergoes functional senescence, resulting in reduced ability to counteract infections and increased frequency of autoimmune and neoplastic diseases.
Article
Pharmacology & Pharmacy
Ana Sofia Agonia, Ana Palmeira-de-Oliveira, Catarina Cardoso, Catia Augusto, Christian Pellevoisin, Christelle Videau, Ricardo Jorge Dinis-Oliveira, Rita Palmeira-de-Oliveira
Summary: The use of in vitro human skin permeation tests is valuable for evaluating the quality and equivalence of topical drug products. In this study, the use of reconstructed human epidermis (RhE) as a biological membrane for bioequivalence testing of clotrimazole creams demonstrated its discriminatory power and higher permeability compared to human skin.
Article
Environmental Sciences
Jee-hyun Hwang, Seungmi Lee, Ho Geon Lee, Dalwoong Choi, Kyung-Min Lim
Summary: The effects of acidic pH on skin need to consider both pH and concentration. Organic and inorganic acids can be irritants to the skin at certain concentrations. Alkaline agents also cause damage to the skin.
Article
Pharmacology & Pharmacy
Anna Domaszewska-Szostek, Magdalena Gewartowska, Marek Stanczyk, Beata Narowska, Maria Moscicka-Wesolowska, Waldemar Lech Olszewski
Summary: Research shows that skin preserved in anhydrous sodium chloride can serve as a source for keratinocyte transplantation for treating extensive skin wounds. Transplanted keratinocytes maintained their morphology and functionality.
Article
Dermatology
Alec Sevilla, Jeremy Cheret, Wendy Lee, Ralf Paus
Summary: This pilot study aimed to investigate the effects of melatonin on melanin production and melanocytes in intact human epidermis. The results showed that melanin content and melanocyte dendricity increased with melatonin treatment, while tyrosinase activity was inhibited. Higher doses of melatonin increased the number and proliferation of melanocytes and keratinocytes, as well as the expression of the premelanosomal marker gp100. These findings suggest that long-term melatonin therapy may stimulate melanogenesis and increase the activity of epidermal melanocytes.
EXPERIMENTAL DERMATOLOGY
(2023)
Article
Cell Biology
Oliver J. Culley, Blaise Louis, Christina Philippeos, Benedicte Oules, Matthieu Tihy, Joe M. Segal, Della Hyliands, Gail Jenkins, Ranjit K. Bhogal, Richard C. Siow, Fiona M. Watt
Summary: Although human dermis contains distinct fibroblast subpopulations, the functional heterogeneity of fibroblast lines from different donors is under-appreciated. We have identified IGF1 and WNT4 as candidate mediators of two distinct dermal functions: myofibroblast formation and epidermal maintenance.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Chemistry, Analytical
Manuel Chacon, Natalia Vazquez, Sergio Alonso-Alonso, Mairobi Persinal-Medina, Sara Llames, Marta Pevida, Ignacio Alcalde, Jesus Merayo-Lloves, Alvaro Meana
Summary: The rabbit skin irritation test has been the standard for evaluating chemical irritation potential, but alternative methods are encouraged. Reconstructed human epidermis (RhE) models mimic human epidermis and can be used as an alternative. As the use of RhE models increases, new challenges have emerged, such as the need for non-invasive and marker-free methodologies. Electrochemical impedance spectroscopy (EIS) is a promising method to meet these requirements.
Article
Multidisciplinary Sciences
Pau-Ring Wong, Nur Fatin Nabilah Mohd Sahardi, Jen-Kit Tan, Kien-Hui Chua, Wan Zurinah Wan Ngah, Suzana Makpol
Summary: This study successfully isolated keratinocytes, fibroblasts, and melanocytes from human foreskin and characterized their purity using gene markers.
Article
Cell & Tissue Engineering
Andrea Tenorio-Mina, Daniel Cortes, Joel Esquivel-Estudillo, Adolfo Lopez-Ornelas, Alejandro Cabrera-Wrooman, Rolando Lara-Rodarte, Itzel Escobedo-Avila, Fernanda Vargas-Romero, Diana Toledo-Hernandez, Enrique Estudillo, Juan Jose Acevedo-Fernandez, Jesus Santa-Olalla Tapia, Ivan Velasco
Summary: Studies have shown that human keratinocytes can express neural cell characteristic genes after culturing in neural medium, but in vitro differentiation did not yield neuronal or astrocytic markers. However, when these cells were grafted into the developing CNS of rats, both control and neural-induced cells expressed neuronal proteins, suggesting the in vivo environment provides appropriate signals for evaluating the neuronal differentiation potential of keratinocytes or other non-neural cell populations.
CELL TRANSPLANTATION
(2021)
Article
Biology
James D. B. O'Sullivan, Carina Nicu, Martin Picard, Jeremy Cheret, Barbara Bedogni, Desmond J. Tobin, Ralf Paus
Summary: Hair greying is a complex process influenced by various factors including genetics, oxidative damage, and melanocyte senescence. The decline in melanogenesis is a key event in the initiation of greying, eventually leading to irreversible loss of melanocytes in the hair follicle. The role of genetic contributions and oxidative damage in driving greying remains unclear, and further research is needed to understand how factors such as hormonal regulation and psychosocial stress affect the process.
BIOLOGICAL REVIEWS
(2021)
Article
Surgery
Renata Migliardi, Alessandra Modugno, Fabrizio Chirico, Nicola Zerbinati, Giovanni Francesco Nicoletti, Giorgio Lo Giudice, Raffaele Rauso
Summary: Fillers can be a safe and versatile method for treating volume deficits in the upper and lower eyelids, projecting the brow arcade, and reducing asymmetry in patients with post-enucleation or evisceration socket syndrome.
EUROPEAN JOURNAL OF PLASTIC SURGERY
(2022)
Review
Dentistry, Oral Surgery & Medicine
R. Rauso, G. Tartaro, G. F. Nicoletti, R. Fragola, G. Lo Giudice, M. Santagata
Summary: This scoping review aims to classify the different alar cinch suture techniques used in orthognathic surgery and propose a standardized classification to avoid duplicate publishing and set a standard for future studies.
INTERNATIONAL JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY
(2022)
Article
Dermatology
C. Pellegrini, S. Raimondi, L. Di Nardo, P. Ghiorzo, C. Menin, M. A. Manganoni, G. Palmieri, G. Guida, P. Quaglino, I. Stanganelli, D. Massi, L. Pastorino, L. Elefanti, G. Tosti, P. Queirolo, A. Leva, A. Maurichi, M. Rodolfo, M. C. Fargnoli
Summary: The study analyzed major high and intermediate melanoma risk genes in a large multicentre cohort of Italian children and adolescents. Results showed most patients carried MC1R variants, while CDKN2A pathogenic variants were found in 9% of cases. Children under 12 years old more frequently presented Spitzoid histotype, with melanoma located on the head/neck and upper limbs and higher Breslow thickness.
JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY
(2022)
Article
Dermatology
C. S. K. Fuchs, V. K. Ortner, M. Mogensen, A. M. Rossi, G. Pellacani, J. Welzel, K. Mosterd, P. Guitera, L. J. Nayahangan, V. L. Johnsson, M. Haedersdal, M. G. Tolsgaard
Summary: This study establishes a set of OCT image features and preferred terminology for basal cell carcinoma (BCC). The research also defines the target group and key components for a curriculum on OCT imaging of BCC, which will facilitate the implementation of OCT imaging of BCC in clinical and research settings.
JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sabrina Sommatis, Maria Chiara Capillo, Cristina Maccario, Elsa Liga, Giulia Grimaldi, Raffaele Rauso, Pier Luca Bencini, Stefania Guida, Nicola Zerbinati, Roberto Mocchi
Summary: This study focused on the rheological characterization and quality assurance of an anti-acne serum, and the evaluation of its biological effectiveness. The results showed that the serum had solid-like behavior and pseudoplastic properties. The serum was non-cytotoxic and non-irritant, with good antimicrobial and anti-inflammatory activity, indicating its effectiveness for acne-prone skin treatment.
Article
Medicine, General & Internal
Ciro Emiliano Boschetti, Giorgio Lo Giudice, Chiara Spuntarelli, Carmine Apice, Raffaele Rauso, Mario Santagata, Gianpaolo Tartaro, Giuseppe Colella
Summary: This study evaluated the effectiveness of Kabat physical rehabilitation in patients with severe facial nerve palsy after parotid gland surgery. The results showed that Kabat therapy could improve the recovery of patients, especially those with high-grade facial nerve palsy.
Editorial Material
Dermatology
Stefania Guida, Nicola Lippolis, Matteo Giovani, Gioia Pedroni, Giacomo Giovanni Urtis, Giovanni Pellacani, Francesca Farnetani, Marco Manfredini
DERMATOLOGY PRACTICAL & CONCEPTUAL
(2022)
Article
Dermatology
Marco Manfredini, Camilla Chello, Silvana Ciardo, Stefania Guida, Johanna Chester, Claudia Lasagni, Laura Bigi, Francesca Farnetani, Vincenzo Bettoli, Giovanni Pellacani
Summary: In this study, dynamic optical coherence tomography (D-OCT) was used to identify the morphological and vascular features of Hidradenitis suppurativa (HS) nodules and their correlation with patient endotype and risk of disease progression. D-OCT enabled the identification of vascular and morphological aspects characterizing HS nodular inflammatory lesions, and significant differences in D-OCT features were found among different disease endotypes. This has important clinical implications for assessing HS risk of progression, making therapeutic decisions, and monitoring treatment efficacy.
EXPERIMENTAL DERMATOLOGY
(2022)
Article
Dermatology
Claudia Pezzini, Silvana Ciardo, Stefania Guida, Shaniko Kaleci, Johanna Chester, Alice Casari, Marco Manfredini, Caterina Longo, Francesca Farnetani, Ariadna Ortiz Brugues, Giovanni Pellacani
Summary: This study combines high-resolution, non-invasive imaging techniques to analyze age-related skin changes. The results show correlations between clinical manifestations of aging and skin cytoarchitecture observed through imaging. As age advances, certain features such as hyperpigmentation and wrinkles become more pronounced, while the skin's collagen fibers become more disrupted. This research provides valuable insights for future studies on skin aging assessment and treatment effectiveness.
EXPERIMENTAL DERMATOLOGY
(2023)
Article
Polymer Science
Pawel Kubik, Daniela Gallo, Maria Laura Tanda, Jerzy Jankau, Raffaele Rauso, Wojciech Gruszczynski, Aleksandra Pawlowska, Pawel Chrapczynski, Maciej Malinowski, Dariusz Grzanka, Marta Smolinska, Paulina Antosik, Maria-Luiza Piesiakow, Bartlomiej Lukasik, Agnieszka Pawlowska-Kubik, Giorgio Stabile, Stefania Guida, Lukasz Kodlubanski, Tom Decates, Nicola Zerbinati
Summary: The study aimed to test the effect of Neauvia Stimulate on patients with Hashimoto's disease, which is a contraindication for hyaluronic acid fillers and calcium hydroxyapatite biostimulants. Histopathological analysis showed a significant reduction in inflammatory infiltration and T lymphocytes after the procedure, with no effect on antibody levels. Risk analysis confirmed the safety of using hyaluronic acid fillers cross-linked with polyethylene glycol in patients with Hashimoto's disease.
Article
Dermatology
Linda Tognetti, Alessandra Cartocci, Elisa Cinotti, Martina Donghia, Magdalena Zychowska, Elvira Moscarella, Emi Dika, Francesca Farnetani, Stefania Guida, John Paoli, Aimilios Lallas, Danica Tiodorovic, Ignazio Stanganelli, Caterina Longo, Mariano Suppa, Iris Zalaudek, Giuseppe Argenziano, Jean Luc Perrot, Giovanni Rubegni, Gennaro Cataldo, Pietro Rubegni
Summary: Atypical pigmented facial lesions (aPFLs) exhibit similar features clinically and dermoscopically. The 14 dermoscopic features vary among different types of aPFLs based on facial areas. aPFLs on the forehead are more likely to be malignant, while those in the orbital/cheek/nose area are probably benign. Lesions on the nose may show features of both malignancy and benignity, making the features less specific.
EXPERIMENTAL DERMATOLOGY
(2023)
Review
Dermatology
Stefania Guida, Hassan Galadari
Summary: This paper provides an up-to-date overview of the clinical applications of Radiesse (R) in facial aesthetics, highlighting its safety and efficacy. However, more research and treatment guidelines are needed.
INTERNATIONAL JOURNAL OF DERMATOLOGY
(2023)
Article
Medicine, General & Internal
Marco Manfredini, Alberto Sticchi, Nicola Lippolis, Gioia Pedroni, Matteo Giovani, Silvana Ciardo, Camilla Chello, Stefania Guida, Francesca Farnetani, Giovanni Pellacani
Summary: Previous studies have shown that acne-prone skin exhibits microscopic abnormalities such as microcomedos and infundibular hyperkeratosis. In this study, treatment with probiotics and a topical product led to a reduction in both clinical and microscopic acne features. Reflectance confocal microscopy and dynamic optical coherence tomography were effective tools for monitoring subclinical changes in acne-prone skin.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Polymer Science
Sabrina Sommatis, Maria Chiara Capillo, Cristina Maccario, Raffaele Rauso, Edoardo D'Este, Martha Herrera, Mauro Castiglioni, Roberto Mocchi, Nicola Zerbinati
Summary: Recently, the frequent use of alcohol-based hand sanitizers has necessitated a careful evaluation of their stability and effectiveness. This study focused on the characterization and rheological behavior of the hand sanitizers, confirming their good stability in gel formula compared to the liquid formula. The antimicrobial assessment also showed the efficacy of both formulations in vitro and in vivo. Overall, our study presents a valid quality control assessment for ensuring the stability and efficacy of alcohol-based hand sanitizers.