期刊
ISCIENCE
卷 24, 期 5, 页码 -出版社
CELL PRESS
DOI: 10.1016/j.isci.2021.102428
关键词
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资金
- iPS Cell Research Fund
- COVID-19 Private Fund
- Joint Usage/ResearchCenter program of Institute for Frontier Life and Medical Sciences, Kyoto University
- Astellas Foundation for Research on Metabolic Disorders
- Senri Life Science Foundation
- Mitsubishi Foundation
- JST Core Research for Evolutional Science and Technology [JPMJCR20HA]
- apan Agency for Medical Research and Development (AMED) [20fk0108263s0201, 20fk0108518s0401, 20fk0108511h0201]
Genetic differences play a key role in the susceptibility and severity of COVID-19. The use of ACE2-iPS cells can model individual differences in SARS-CoV-2 infection in vitro and help clarify the causes of individual differences in COVID-19. Male iPS/ES cells were found to be more capable of producing the virus compared with female iPS/ES cells in the study.
Genetic differences are a primary reason for differences in the susceptibility and severity of COVID-19. As induced pluripotent stem (iPS) cells maintain the genetic information of the donor, they can be used to model individual differences in SARS-CoV-2 infection in vitro. We found that human iPS cells expressing the SARS-CoV-2 receptor angiotensin-converting enzyme 2 (ACE2) (ACE2-iPS cells) can be infected w SARS-CoV-2. In infected ACE2-iPS cells, the expression of SARS-CoV-2 nucleocapsid protein, budding of viral particles, and production of progeny virus, double membrane spherules, and double-membrane vesicles were confirmed. We performed SARS-CoV-2 infection experiments on ACE2-iPS/ embryonic stem (ES) cells from eight individuals. Male iPS/ES cells were more capable of producing the virus compared with female iPS/ES cells. These findings suggest that ACE2-iPS cells can not only reproduce individual differences in SARS- CoV-2 infection in vitro but also are a useful resource to clarify the causes of individual differences in COVID-19 due to genetic differences.
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