Applications of Bacterial Degrons and Degraders - Toward Targeted Protein Degradation in Bacteria

A repertoire of proteolysis-targeting signals known as degrons is a necessary component of protein homeostasis in every living cell. In bacteria, degrons can be used in place of chemical genetics approaches to interrogate and control protein function. Here, we provide a comprehensive review of synthetic applications of degrons in targeted proteolysis in bacteria. We describe recent advances ranging from large screens employing tunable degradation systems and orthogonal degrons, to sophisticated tools and sensors for imaging. Based on the success of proteolysis-targeting chimeras as an emerging paradigm in cancer drug discovery, we discuss perspectives on using bacterial degraders for studying protein function and as novel antimicrobials.

Automated summary

Degrading signals known as degrons are essential for protein homeostasis in all living cells, and can be utilized in bacteria to manipulate protein function. Recent advancements include large screenings with tunable degradation systems and orthogonal degrons, as well as the development of sophisticated tools and sensors for imaging purposes. The success of proteolysis-targeting chimeras in cancer drug discovery has led to discussions on the potential use of bacterial degraders for studying protein function and as novel antimicrobials.