Article
Pharmacology & Pharmacy
Hojun Choi, Myung-Yoon Kim, Dae-Hwan Kim, Hanoul Yun, Byung-Koo Oh, Su-Bin Kim, In-Ho Song, Hyun-Soo Park, Sang-Eun Kim, Cheolhyoung Park, Chulhee Choi
Summary: This study assessed the biodistribution and pharmacokinetics of therapeutic exosomes labeled with Zr-89 in mice and rats. The results showed that the exosomes were mainly distributed in the liver and spleen, with substantial uptake by parenchymal cells. They were also moderately distributed in the kidney, lung, stomach, intestine, urinary bladder, brain, and heart. Exosomes were rapidly cleared from circulation, indicating their rapid uptake by cells and tissues.
Review
Cell & Tissue Engineering
Hojun Choi, Yoorim Choi, Hwa Young Yim, Amin Mirzaaghasi, Jae-Kwang Yoo, Chulhee Choi
Summary: Exosomes are nano-sized vesicles secreted by cells for intercellular communication, with therapeutic potential when engineered for targeted delivery of active pharmaceutical ingredients. Recent advances have shown the potential for targeted delivery of engineered exosomes to challenging organs, including the central nervous system.Various methods, such as imaging techniques and surface engineering, can modulate the biodistribution and pharmacokinetics of exosomes.
TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2021)
Article
Multidisciplinary Sciences
Zhuoran Ma, Feifei Wang, Weizhi Wang, Yeteng Zhong, Hongjie Dai
Summary: This study utilized artificial neural networks to transform fluorescence images in the shorter-wavelength NIR window to images resembling NIR-IIb window, achieving high signal-to-background ratio in vivo lymph node imaging with human-approved molecular probes. Translation of PD-L1 or EGFR imaging greatly enhanced tumor-to-normal tissue ratio and improved tumor margin localization, showcasing the potential of deep learning in enhancing noninvasive NIR imaging and microscopy. Deep learning equipped NIR imaging could facilitate basic biomedical research and clinical diagnostics and imaging-guided surgery.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Multidisciplinary
Hua Ma, Iris Y. Zhou, Y. Iris Chen, Nicholas J. Rotile, Ilknur Ay, Eman A. Akam, Huan Wang, Rachel S. Knipe, Lida P. Hariri, Caiyuan Zhang, Matthew Drummond, Pamela Pantazopoulos, Brianna F. Moon, Avery T. Boice, Samantha E. Zygmont, Jonah Weigand-Whittier, Mozhdeh Sojoodi, Romer A. Gonzalez-Villalobos, Michael K. Hansen, Kenneth K. Tanabe, Peter Caravan
Summary: We report on three Mn(II)-based small-molecule magnetic resonance probes containing α-effect nucleophiles to target allysine in vivo and detect tissue fibrogenesis. The design approach resulted in turn-on probes with a 4-fold increase in relaxivity upon targeting. The probes' performance in detecting tissue fibrogenesis was evaluated using a systemic aldehyde tracking approach, showing efficiency in characterizing fibrogenesis throughout the lung and liver.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Biochemistry & Molecular Biology
Shiran Su, Thomas J. Esparza, Duong Nguyen, Simone Mastrogiacomo, Joong H. Kim, David L. Brody
Summary: This study aimed to develop imaging methods and pharmacokinetic models to aid in the future development of brain MRI molecular contrast agents. An efficient near-infrared imaging method and a five-compartment PK model were established to monitor and evaluate the dynamics of VHH and VHH-conjugated nanoparticles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Amir Sadeghi, Marika Ruponen, Jooseppi Puranen, Shoupeng Cao, Roxane Ridolfo, Shirin Tavakoli, Elisa Toropainen, Tatu Lajunen, Veli-Pekka Ranta, Jan van Hest, Arto Urtti
Summary: This study investigates the pharmacokinetics of nanomaterials in the eyes of rats and rabbits. The impact of particle size and shape on ocular distribution and elimination was examined using various imaging techniques. The results show that differently sized particles have different retention times and elimination rates, suggesting that factors other than size also play a role in their kinetics. The study also demonstrates the importance of synchronized particle retention and drug release rate for efficient drug delivery.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Chemistry, Multidisciplinary
Kaichao Song, Yumei Hao, Xiaochuan Tan, Hongdong Huang, Lulu Wang, Wensheng Zheng
Summary: This study developed microneedles (MNs) by fusing borneol-modified liposomes (LIPs) with exosomes from mesenchymal stem cells (MSCs) and loading them with Ziconotide (ZIC), aiming to improve the delivery efficiency of ZIC across the blood-brain barrier. The results demonstrated that ZIC-loaded MNs had significant analgesic effects in different pain models.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Biochemistry & Molecular Biology
Sakkarapalayam M. Mahalingam, Karson S. Putt, Madduri Srinivasarao, Philip S. Low
Summary: The inadvertent severing of a ureter during colorectal surgeries is a common issue, leading to the development of new near-infrared dyes to help surgeons identify ureter location. The new PEGylated UreterGlow derivatives show promising optical and biodistribution characteristics, making them good candidates for translation into the clinic.
Article
Chemistry, Multidisciplinary
Irene Lim, Eric Yu Lin, Joseph Garcia, Shang Jia, Robert E. Sommerhalter, Subrata K. Ghosh, John A. Gladysz, Ellen M. Sletten
Summary: Developing chemical tools to detect and influence biological processes is important in chemical biology. In this study, we combined perfluorocarbons and multiplexed shortwave infrared fluorescence imaging to visualize nanoemulsions in real time in living mice. We prepared two fluorous-soluble chromenylium polymethine dyes that emit shortwave infrared light and successfully tracked the biodistribution of perfluorocarbon nanoemulsions in vivo. Our findings provided insight into the significance of size and surfactant identity on biodistribution.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Pharmacology & Pharmacy
Thomashire A. George, Chuan-Chih Hsu, Annette Meeson, David J. Lundy
Summary: This review summarizes the principles, developments, and challenges of nanocarriers for myocardial infarction therapy, with a focus on ligand-based and cell mimicry-based targeting. It provides guidance and insights for further research and clinical applications.
Review
Pharmacology & Pharmacy
Yuki Takahashi, Yoshinobu Takakura
Summary: Extracellular vesicles (EVs) are cell-derived membrane vesicles composed of a lipid bilayer that contain biological molecules. They function as intercellular communication tools and have potential as therapeutic targets for various diseases. However, an EV-based drug delivery system utilizing their therapeutic properties has not yet been reported.
PHARMACOLOGY & THERAPEUTICS
(2023)
Article
Biotechnology & Applied Microbiology
Rita M. Pinto, Saleh Yazdani, Catarina Leal Seabra, Martine De Jonge, Mukaddes Izci, Rebeca Cruz, Susana Casal, Stefaan J. Soenen, Salette Reis, Claudia Nunes, Patrick Van Dijck
Summary: Staphylococcus aureus is a high priority pathogen due to its prevalence and ability to form biofilms. Current treatments do not target the matrix that contributes to antimicrobial tolerance. This study proposes the use of lipid nanoparticles encapsulating caspofungin to disrupt the matrix, and combining them with moxifloxacin-loaded nanoparticles to reduce biofilms. In vitro and in vivo studies showed promising results, with reduced biofilm formation and no bacterial dissemination into vital organs.
NPJ BIOFILMS AND MICROBIOMES
(2023)
Article
Chemistry, Multidisciplinary
Julia Ackermann, Jan Stegemann, Tim Smola, Eline Reger, Sebastian Jung, Anne Schmitz, Svenja Herbertz, Luise Erpenbeck, Karsten Seidl, Sebastian Kruss
Summary: Optical nanosensors based on single-wall carbon nanotubes are able to capture the dynamics of biochemical processes. A one-step phase separation approach has been developed to isolate monochiral (6,4)-SWCNTs from mixed SWCNT samples, enabling high-resolution imaging in the near-infrared spectrum using standard Si-based cameras. (6,4)-SWCNTs modified with (GT)(10)-ssDNA become highly sensitive to the neurotransmitter dopamine, allowing for fast and high-resolution imaging of dopamine release from cells.
Article
Engineering, Biomedical
Zixin Sun, Xiaohui Chen, Ruyan Niu, Hengyi Chen, Ying Zhu, Chong Zhang, Liu Wang, Huaming Mou, Hong Zhang, Yang Luo
Summary: This study developed a Liposome Fusogenic Enzyme-free circuit (LIFE) approach that enables accurate identification of single exosomal inclusions without destroying the vesicle integrity. The probe-loaded cationic fusogenic liposome can capture and fuse with a target exosome, allowing for probes delivery and target biomolecule-initiated cascaded signal amplification. By controlling the ratio of the introduced LIFE probe, trace cargoes in a single exosome can be accurately determined, paving the way for a universal sensing platform to assess exosomal cargoes for early disease diagnosis and personalized treatment.
MATERIALS TODAY BIO
(2023)
Article
Engineering, Civil
Ewa M. Nowara, Tim K. Marks, Hassan Mansour, Ashok Veeraraghavan
Summary: Imaging photoplethysmography (iPPG) has the potential to enhance driver safety systems by detecting driver fatigue and early heart failure in a non-invasive manner. However, iPPG faces challenges in the driving context due to illumination and motion. This paper proposes two innovations to overcome these challenges: using narrow-band near-infrared video recordings to reduce illumination variations, and introducing a novel optimization algorithm called AutoSparsePPG that improves performance compared to existing methods.
IEEE TRANSACTIONS ON INTELLIGENT TRANSPORTATION SYSTEMS
(2022)