Development of dual‐inducible duet‐expression vectors for tunable gene expression control and CRISPR interference‐based gene repression in Pseudomonas putida KT2440

An expanded CRISPRi toolbox for tunable control of gene expression in Pseudomonas putida

(2020) Christos Batianis et al.   Microbial Biotechnology

Construction of a novel dual-inducible duet-expression system for gene (over)expression in Pseudomonas putida

(2020) Rahul Gauttam et al.   PLASMID

Genome-scale metabolic rewiring improves titers rates and yields of the non-native product indigoidine at scale

(2020) Deepanwita Banerjee et al.   Nature Communications

Physical decoupling of XylS/Pm regulatory elements and conditional proteolysis enable precise control of gene expression in Pseudomonas putida

(2019) Daniel C. Volke et al.   Microbial Biotechnology

Accelerated genome engineering of Pseudomonas putida by I-Sce I―mediated recombination and CRISPR-Cas9 counterselection

(2019) Nicolas T. Wirth et al.   Microbial Biotechnology

CRISPR interference-mediated gene regulation in Pseudomonas putida KT2440

(2019) Seong Keun Kim et al.   Microbial Biotechnology

Redirecting Metabolic Flux via Combinatorial Multiplex CRISPRi-Mediated Repression for Isopentenol Production in Escherichia coli

(2019) Tian Tian et al.   ACS Synthetic Biology

Systems Metabolic Engineering Strategies: Integrating Systems and Synthetic Biology with Metabolic Engineering

(2019) Kyeong Rok Choi et al.   TRENDS IN BIOTECHNOLOGY

A simple dual-inducible CRISPR interference system for multiple gene targeting in Corynebacterium glutamicum

(2019) Rahul Gauttam et al.   PLASMID

New tools for recombinant protein production in Escherichia coli : A 5-year update

(2019) Germán L. Rosano et al.   PROTEIN SCIENCE

Pseudomonas putida in the quest of programmable chemistry

(2019) Esteban Martínez-García et al.   CURRENT OPINION IN BIOTECHNOLOGY

Genetic tools for reliable gene expression and recombineering in Pseudomonas putida

(2018) Taylor B. Cook et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Pseudomonas putida as a functional chassis for industrial biocatalysis: From native biochemistry to trans -metabolism

(2018) Pablo I. Nikel et al.   METABOLIC ENGINEERING

Biological standards for the Knowledge-Based BioEconomy: What is at stake

(2018) Víctor de Lorenzo et al.   New Biotechnology

Chasing bacterial chassis for metabolic engineering: a perspective review from classical to non-traditional microorganisms

(2018) Patricia Calero et al.   Microbial Biotechnology

The art of vector engineering: towards the construction of next-generation genetic tools

(2018) Luísa Czamanski Nora et al.   Microbial Biotechnology

High level in vivo mucin-type glycosylation in Escherichia coli

(2018) Phillipp Mueller et al.   Microbial Cell Factories

A step forward: Compatible and dual-inducible expression vectors for gene co-expression in Corynebacterium glutamicum

(2018) Rahul Gauttam et al.   PLASMID

Construction of pDUO: A bicistronic shuttle vector series for dual expression of recombinant proteins

(2017) Paul A. Nakata   PLASMID

Characterisation of a 3-hydroxypropionic acid-inducible system from Pseudomonas putida for orthogonal gene expression control in Escherichia coli and Cupriavidus necator

(2017) Erik K. R. Hanko et al.   Scientific Reports

CRISPR/Cas9-Based Counterselection Boosts Recombineering Efficiency in Pseudomonas putida

(2017) Tomás Aparicio et al.   Biotechnology Journal

Optimizing sgRNA position markedly improves the efficiency of CRISPR/dCas9-mediated transcriptional repression

(2016) Aliaksandra Radzisheuskaya et al.   NUCLEIC ACIDS RESEARCH

Broad-Host-Range ProUSER Vectors Enable Fast Characterization of Inducible Promoters and Optimization of p-Coumaric Acid Production in Pseudomonas putida KT2440

(2016) Patricia Calero et al.   ACS Synthetic Biology

Metabolic Engineering of Corynebacterium glutamicum for Methanol Metabolism

(2015) Sabrina Witthoff et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Metabolic Engineering of Pseudomonas putida KT2440 to Produce Anthranilate from Glucose

(2015) Jannis Kuepper et al.   Frontiers in Microbiology

Functional Characterization of the Mannitol Promoter of Pseudomonas fluorescens DSM 50106 and Its Application for a Mannitol-Inducible Expression System for Pseudomonas putida KT2440

(2015) Jana Hoffmann et al.   PLoS One

SEVA 2.0: an update of the Standard European Vector Architecture for de-/re-construction of bacterial functionalities

(2014) Esteban Martínez-García et al.   NUCLEIC ACIDS RESEARCH

Functional characterization and application of a tightly regulated MekR/P mekA expression system in Escherichia coli and Pseudomonas putida

(2013) Nadja Graf et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Development of a broad-host synthetic biology toolbox for ralstonia eutropha and its application to engineering hydrocarbon biofuel production

(2013) Changhao Bi et al.   Microbial Cell Factories

Heterologous Expression and Genetic Engineering of the Tubulysin Biosynthetic Gene Cluster Using Red/ET Recombineering and Inactivation Mutagenesis

(2012) Yi Chai et al.   CHEMISTRY & BIOLOGY

The Standard European Vector Architecture (SEVA): a coherent platform for the analysis and deployment of complex prokaryotic phenotypes

(2012) Rafael Silva-Rocha et al.   NUCLEIC ACIDS RESEARCH

BglBrick vectors and datasheets: A synthetic biology platform for gene expression

(2011) Taek Lee et al.   Journal of Biological Engineering

New yeast recombineering tools for bacteria

(2009) Robert M.Q. Shanks et al.   PLASMID