Article
Biochemistry & Molecular Biology
Burcu Sarikaya, Menemse Gumusderelioglu
Summary: The research showed that optimized SF/P3HB scaffolds supported adipose-derived mesenchymal stem cells to proliferate and differentiate into tenocytes, regardless of the absence of tenogenic differentiation factor-5 (GDF-5). The scaffolds also exhibited bacteriostatic properties.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Anatomy & Morphology
Negar Azarpira, Maryam Kaviani, Fatemeh Sabet Sarvestani
Summary: The study demonstrated the potential of alginate oxide particles in promoting angiogenesis through the immobilization and controlled release of VEGF and bFGF. In vitro experiments showed that VEGF-loaded particles could enhance Total Branching Points and maintain the viability of human umbilical vein endothelial cells in the constructs.
Article
Biochemistry & Molecular Biology
Nouf N. Mahmoud, Zain Zaki Zakaria, Hadeel Kheraldine, Ishita Gupta, Semir Vranic, Maha Al-Asmakh, Ala-Eddin Al Moustafa
Summary: The study shows that surface-modified gold nanorods have differential effects on avian embryos survival and blood vessel formation. There is a high mortality rate in embryos exposed to PEGylated phospholipid-modified nanorods, while PEGylated cholesterol-modified nanorods inhibit angiogenesis. Moreover, the modified nanorods disrupt the expression of genes involved in various cellular processes and modulate specific molecular pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Hend M. Nawara, Said M. Afify, Ghmkin Hassan, Maram H. Zahra, Marwa N. Atallah, Akimasa Seno, Masaharu Seno
Summary: CSCs play a crucial role in tumor growth and metastasis, with their angiogenic potential successfully evaluated through the CAM assay, proving to be an effective method for assessing antiangiogenic drugs.
CELL BIOLOGY INTERNATIONAL
(2021)
Article
Pharmacology & Pharmacy
Pan Zhang, Xing Lai, Mao-Hua Zhu, Mei Long, Xue-Liang Liu, Zi-Xiang Wang, Yifan Zhang, Run-Jie Guo, Jing Dong, Qin Lu, Peng Sun, Chao Fang, Mei Zhao
Summary: Saikosaponin A (SSA) has been shown to suppress proliferation, migration, and tube formation of endothelial cells, as well as inhibit angiogenesis in various models. It was found to inhibit tumor growth by blocking the VEGFR2-mediated signaling pathway.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Zahra Bashiri, Ali Moghaddaszadeh, Reza Falak, Farnaz Khadivi, Azita Afzali, Mehdi Abbasi, Ali Mohammad Sharifi, Hamid Reza Asgari, Farid Ghanbari, Morteza Koruji
Summary: In this study, a biocompatible porous scaffold based on a triad mixture of silk fibroin (SF), alginate (Alg), and laminin (LM) is developed to facilitate the differentiation of mouse spermatogonia stem cells (SSCs). The composite scaffolds showed improved structural stability and mechanical properties compared to homogenous structures, and the cells developed in the composite scaffolds exhibited significantly higher expression of differentiation-related proteins.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Plant Sciences
Dan Wang, Miao Qi, Hedi Zhao, Haozhong Wu, Han Chen, Yanan Lan, Yanmin Wang, Yani Jiang, Jingjuan Wang
Summary: The aim of this study was to determine the processing temperatures at which Coptis chinensis Franch. (CC) has the strongest anti-angiogenic effect and to speculate the possible bioactive components. The results showed that CC at different processing temperatures and its seven alkaloids could inhibit angiogenesis, with the most significant effect observed in CC processed at 40 degrees Celsius and palmatine. LC-MS analysis revealed that palmatine displayed the strongest inhibitory effect on angiogenesis. Therefore, this study suggests that the q-CAM model in conjunction with UPLC-MS/MS could be a useful tool for assessing tumor angiogenesis and screening tumor-targeted medicines. Processing temperature can affect the anti-angiogenic effect of CC, and palmatine can be considered as a prospective anti-angiogenic drug.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Cell Biology
Dovydas Gecys, Ruta Skredeniene, Emilija Gecyte, Arunas Kazlauskas, Ingrida Balnyte, Aiste Jekabsone
Summary: Adipose tissue-derived mesenchymal stem cell extracellular vesicles (ASC-EVs) possess anti-tumor properties, reducing glioblastoma cell proliferation and invasiveness, and can be applied as anticancer therapeutics and medicine carriers.
Article
Surgery
Eric Pion, Isabel Zucal, Johannes Troebs, Anna-Lena Feder, Thura Kyaw, Sopyay Khin, Paul Heidekrueger, Lukas Prantl, Silke Haerteis, Thiha Aung
Summary: This study utilized the chorioallantoic membrane model as an innovative and versatile training model for microsurgery and supermicrosurgery, which can reduce the use of animals. The model accurately simulates the anatomical and physiological features and has a high variety of vascular structures.
PLASTIC AND RECONSTRUCTIVE SURGERY
(2022)
Article
Biotechnology & Applied Microbiology
Xiaoyin Liu, Guijun Zhang, Pan Wei, Lifang Hao, Lin Zhong, Kunhon Zhong, Chang Liu, Peng Liu, Qingbo Feng, Shan Wang, Jianyong Zhang, Rui Tian, Liangxue Zhou
Summary: This study prepared a 3D-printed collagen/silk fibroin/secretome scaffold derived from human umbilical cord blood mesenchymal stem cells (HUCMSCs) pretreated with bFGF at a low temperature, aiming to promote brain tissue regeneration after traumatic brain injury (TBI). In vitro experiments showed that the 3D-CS-bFGF-ST scaffold exhibited good biodegradation, suitable mechanical properties, and biocompatibility. In vivo experiments demonstrated that the 3D-CS-bFGF-ST group had significantly faster brain tissue regeneration compared to the other two groups, as evidenced by motor assay, histological analysis, and immunofluorescence assay. The 3D-CS-bFGF-ST group achieved a better outcome than the 3D-CS-ST group at 6 months postsurgery.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Polymer Science
Ponrasu Thangavel, Hemalatha Kanniyappan, Sudip Chakraborty, Shipra Chaudhary, Aadinath Wallepure, Vignesh Muthuvijayan
Summary: Biomimetic scaffolds were prepared using konjac glucomannan (KGM) and silk fibroin (SF), which were cross-linked with citric acid and lyophilized to obtain fibrous structures. The KGM/SF scaffolds exhibited improved thermal stability, porous morphology, and biodegradable nature. They also showed enhanced compression strength and demonstrated biocompatibility, cell viability, and vascularization efficacy in tissue engineering and biomedical applications.
JOURNAL OF APPLIED POLYMER SCIENCE
(2023)
Review
Pathology
Domenico Ribatti, Vincenzo Benagiano
Summary: This review article discusses the potential use of the chick embryo area vasculosa as an in vivo assay for studying both angiogenesis and anti-angiogenesis. It suggests that this alternative method can be more useful than the chorioallantoic membrane in studying tumor growth, angiogenesis, and metastasis, as well as the angiogenic activity of acellular scaffolds and organoids.
PATHOLOGY RESEARCH AND PRACTICE
(2023)
Article
Polymer Science
Peng Pan, Cheng Hu, Ahui Liang, Xueping Liu, Mengqi Fang, Shanlong Yang, Yadong Zhang, Mingzhong Li
Summary: The study developed a hybrid scaffold with antibacterial and healing-guiding functions. Results showed that the scaffold exhibited significant antibacterial activity against bacteria when the PHMB/SF ratio was greater than 1/100, and promoted cell adhesion, spreading, and proliferation when the ratio was less than 2/100.
Article
Biochemistry & Molecular Biology
Selvaraj Vimalraj, Suresh Renugaa, Anuradha Dhanasekaran
Summary: This paper introduces the chick embryo chorioallantoic membrane as a platform for evaluating the biological characteristics of biomaterials, and explores its potential applications in the field of tissue engineering.
PROCESS BIOCHEMISTRY
(2023)
Article
Peripheral Vascular Disease
Domenico Ribatti
Summary: This review article summarizes the literature data on the two new applications of the chick embryo chorioallantoic membrane assay, highlighting its advantages.
MICROVASCULAR RESEARCH
(2022)
Article
Developmental Biology
Fiona Bangs, Nicole Antonio, Peerapat Thongnuek, Monique Welten, Megan G. Davey, James Briscoe, Cheryll Tickle
Article
Materials Science, Multidisciplinary
Juthatip Manissorn, Pimnara Wattanachai, Khaow Tonsomboon, Palang Bumroongsakulsawat, Siriporn Damrongsakkul, Peerapat Thongnuek
Summary: By crosslinking silk fibroin polypeptides with bioactive glass using GPTMS during sol-gel process, the developed hybrid scaffolds showed improved long-term stability and bioactivity. This new type of scaffold demonstrated retarded enzymatic degradation, enhanced mechanical integrity, and induced osteoblast proliferation and expression of osteogenic markers.
MATERIALS TODAY COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Juthatip Manissorn, Thassanai Sitthiyotha, Jenny Rose E. Montalban, Surasak Chunsrivirot, Peerapat Thongnuek, Kittikhun Wangkanont
ACS CHEMICAL BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Anongnart Duangpakdee, Chavee Laomeephol, Depicha Jindatip, Peerapat Thongnuek, Juthamas Ratanavaraporn, Siriporn Damrongsakkul
Summary: The study developed 3D porous scaffolds fabricated from blends of silk fibroin, gelatin, and hyaluronan, which showed water stability and enhanced swellability. The scaffolds supported cell adhesion and proliferation, had high porosity, interconnectivity, and observed cell penetration into the structures.
Article
Chemistry, Multidisciplinary
Juthatip Manissorn, Khaow Tonsomboon, Kittikhun Wangkanont, Peerapat Thongnuek
Summary: Bone morphogenetic protein-2 (BMP-2) is commonly expressed in Escherichia coli as inclusion bodies due to its high yield and low cost. The refolding process is a bottleneck for BMP-2 production. This study investigated the effects of the refolding buffer composition on BMP-2 refolding and found that the highest yield of BMP-2 dimers, the biologically active form, was observed in a refolding buffer containing ionic detergents. Additionally, the combination of sugars and anionic detergents reduced BMP-2 oligomers and increased the BMP-2 dimer yield.
Article
Materials Science, Biomaterials
Jiranuwat Sapudom, Mesayamas Kongsema, Apipon Methachittipan, Siriporn Damrongsakkul, Sorada Kanokpanont, Jeremy C. M. Teo, Mattaka Khongkow, Khaow Tonsomboon, Peerapat Thongnuek
Summary: Silk fibroin (SF) scaffolds, used as functional materials for tissue engineering and implantation, were studied for their degradation products' effect on host response. Two different crosslinkers (EDC and GA) were used to enhance stability and degradation of SF scaffolds. The degradation products of SF scaffolds were found to mediate immune modulation of macrophages, promoting pro-inflammatory or anti-inflammatory phenotypes.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
