Article
Cell & Tissue Engineering
Ihsene Taihi, Caroline Pilon, Jose Cohen, Ariane Berdal, Bruno Gogly, Ali Nassif, Benjamin Philippe Fournier
Summary: A new serum-free medium enriched with platelet lysate has been developed for the isolation and proliferation of gingival stem cells. This medium enhances osteogenic capacity and maintains immunomodulatory properties, making it suitable for use in human cell therapy for bone regeneration according to good manufacturing practice guidelines.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Alice Blufstein, Christian Behm, Barbara Kubin, Johannes Gahn, Andreas Moritz, Xiaohui Rausch-Fan, Oleh Andrukhov
Summary: This study reveals the immunomodulatory properties of GMSCs towards PMNs, with IL-1 beta enhancing their anti-apoptotic effects. However, GMSCs did not affect the ROS production of PMNs.
Article
Biochemistry & Molecular Biology
Alasdair G. Kay, Kane Treadwell, Paul Roach, Rebecca Morgan, Rhys Lodge, Mairead Hyland, Anna M. Piccinini, Nicholas R. Forsyth, Oksana Kehoe
Summary: This study evaluated the therapeutic potential of extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) in an antigen-induced model of arthritis. The results showed that these EVs reduced knee-joint swelling, improved histopathological outcomes, and regulated T cell polarization. Among the different conditions tested, EVs cultured under normoxic conditions demonstrated the optimal therapeutic effect.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Immunology
Elzbieta Karnas, Patrycja Dudek, Ewa K. Zuba-Surma
Summary: In recent years, the use of stem cells in regenerative medicine has gained attention for their ability to proliferate and differentiate. However, it has been found that stem cells can also secrete extracellular vesicles (EVs) with biologically active factors that can modulate the activity of recipient cells and regulate inflammation. The potential therapeutic applications of EVs in inflammatory-related dysfunctions are being explored as an alternative to traditional cell-based approaches. This review discusses the biological properties of SC-derived EVs and their role in the regulation of inflammatory response, as well as recent findings on their immunomodulatory and pro-regenerative activity in various disease models.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Oscar O. Solis-Castro, Marcelo N. Rivolta, Fiona M. Boissonade
Summary: Evidence suggests that dental-related stem cells (DRSCs) have the potential for ectodermal lineage differentiation and can be used in the treatment of neurodegenerative diseases and nerve injuries. However, there are limitations in current research, such as the reliance on detecting neural crest markers to classify DRSCs as NCSCs and the lack of studies proving their improved neuro-glial differentiation or applicability in relevant models. Furthermore, existing protocols need to meet manufacturing standards for clinical applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Retraction
Biochemistry & Molecular Biology
Sabine Wislet-Gendebien, Emerence Laudet, Virginie Neirinckx, Philippe Alix, Pierre Leprince, Aneta Glejzer, Christophe Poulet, Benoit Hennuy, Lukas Sommer, Olga Shakhova, Bernard Rogister
Summary: A correction to this paper has been published.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Leah C. Ott, Christopher Y. Han, Jessica L. Mueller, Ahmed A. Rahman, Ryo Hotta, Allan M. Goldstein, Rhian Stavely
Summary: Neurons and glia of the peripheral nervous system are derived from progenitor cell populations originating from embryonic neural crest, and these cells have previously been shown to possess neural stem cell qualities. In this study, a population of neural crest-derived Schwann cells residing in the bone marrow, in association with nerve fibers, were found to exhibit plasticity and generate neural stem cells with neurogenic potential. These cells can form neural networks within the enteric nervous system following transplantation, making them a novel source of autologous neural stem cells for the treatment of neurointestinal disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Gina D. Kusuma, Anqi Li, Dandan Zhu, Hannah McDonald, Ishmael M. Inocencio, Daniel C. Chambers, Kenneth Sinclair, Haoyun Fang, David W. Greening, Jessica E. Frith, Rebecca Lim
Summary: The impact of 3D culture on the secretion, proteome composition, and functional properties of extracellular vesicles (EVs) from mesenchymal stem cells (MSCs) was evaluated in this study. The results showed that EVs from 3D culture did not enhance typical MSC-EV properties expected from 2D culture, such as immunomodulation, anti-fibrotic, and anti-inflammatory effects. The study highlighted the critical differences between MSC-EVs obtained from different culture microenvironments, which should be considered in clinical manufacturing.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Cell Biology
Maria I. Quinones-Vico, Raquel Sanabria de la Torre, Manuel Sanchez-Diaz, Alvaro Sierra-Sanchez, Trinidad Montero-Vilchez, Ana Fernandez-Gonzalez, Salvador Arias-Santiago
Summary: The skin, the largest organ of the human body, has important functions including protection and maintenance. MSCs and their secretome, particularly exosomes, are believed to play a significant role in skin repair and regeneration, indicating the need for further research for clinical translation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Oncology
Adrienne M. Parsons, Eric M. Darling
Summary: The study revealed the impact of time on adipose-derived stem/stromal cell (ASC) response to an inflammatory environment. Time played a role in the balance of pro- and anti-inflammatory ASC phenotypes, with IL-6 consistently present and IDO1 heterogeneously upregulated in later stages of sustained cytokine stimulation. Immune response of ASC remained constant for 96-168 hours after a pulse stimulus, and the presence of ASCs altered macrophage phenotype independent of ASC cytokine exposure time.
EXPERIMENTAL CELL RESEARCH
(2021)
Review
Cell Biology
Anna L. Hoeving, Beatrice A. Windmoeller, Cornelius Knabbe, Barbara Kaltschmidt, Christian Kaltschmidt, Johannes F. W. Greiner
Summary: The review highlights the cellular heterogeneity within neural crest-derived stem cell populations and emphasizes this heterogeneity as a crucial parameter for understanding their role in tissue homeostasis and regeneration.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Songyue Lou, Yongtao Duan, Huizong Nie, Xujie Cui, Jialing Du, Yongfang Yao
Summary: Mesenchymal stem cells (MSCs) have immunomodulatory functions and transplantation potential, making them suitable for the treatment of various diseases. They have broad clinical application prospects in tissue regeneration, immunomodulation, and oncology.
Article
Multidisciplinary Sciences
Kosar Malekpour, Ali Hazrati, Sara Soudi, Leila Roshangar, Ali Akbar Pourfathollah, Majid Ahmadi
Summary: Diabetes is a common metabolic disorder associated with insulin resistance, which develops years before diabetes onset. Insulin resistance leads to complications and liver inflammation, which can progress to severe liver conditions. Metformin is the primary treatment for diabetes, but it has side effects. This study investigated the potential of combining metformin with MSCs-derived exosomes to enhance its effectiveness in reducing inflammation and apoptosis in liver cells.
Article
Biochemistry & Molecular Biology
Xishuai Wang, Shiyu Zhao, Junhui Lai, Weijun Guan, Yang Gao
Summary: This study demonstrates that gingival-derived mesenchymal stem cells (GMSCs) intervene and protect against bleomycin-induced pulmonary fibrosis, while exhibiting anti-inflammatory and antioxidant capacities.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
Michal Domaniza, Marian Hluchy, Dasa Cizkova, Filip Humenik, Lucia Slovinska, Nikola Hudakova, Lubica Hornakova, Juraj Vozar, Alexandra Trbolova
Summary: This study aimed to investigate the potential of cell-based regenerative therapy for elbow joints affected by osteoarthritis. The results showed that two injections of amnion-derived mesenchymal stem cells improved joint range of motion and had an impact on synovial fluid biomarkers. Concentrations of IFN-γ, IL-6, and TNF-α decreased while IL-10 increased between day 0 and day 28 after treatment.