期刊
JOURNAL OF NANOBIOTECHNOLOGY
卷 19, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s12951-021-00887-4
关键词
Electro-optical transduction; Screen-printed electrodes; Paper-based biosensors; GRAS organisms; Eco-friendly
资金
- ALGAE-CB Bilateral Project Italy-Morocco 2018/2019 within the Joint Research Projects Programme of the Italian National Research Council
- NanoSWS project EraNetMed [RQ3-2016]
- AdSWiM Interreg Project Italy-Croatia
The development of a dual electro-optical biosensor for herbicide monitoring based on algal biosensors is reported in this study. A systematic study was conducted to select and characterize a group of 28 different genetic variants of the alga, with the CC125 strain chosen as a case study. The biosensor demonstrated high sensitivity, repeatability, long-lasting working time, and storage stability in standard solutions and real samples, with low interference in the presence of heavy metals and insecticides.
The indiscriminate use of herbicides in agriculture contributes to soil and water pollution, with important endangering consequences on the ecosystems. Among the available analytical systems, algal biosensors have demonstrated to be valid tools thanks to their high sensitivity, cost-effectiveness, and eco-design. Herein, we report the development of a dual electro-optical biosensor for herbicide monitoring, based on Chlamydomonas reinhardtii whole cells immobilised on paper-based screen-printed electrodes modified with carbon black nanomaterials. To this aim, a systematic study was performed for the selection and characterisation of a collection among 28 different genetic variants of the alga with difference response behaviour towards diverse herbicide classes. Thus, CC125 strain was exploited as case study for the study of the analytical parameters. The biosensor was tested in standard solutions and real samples, providing high sensitivity (detection limit in the pico/nanomolar), high repeatability (RSD of 5% with n=100), long lasting working (10 h) and storage stability (3 weeks), any interference in the presence of heavy metals and insecticides, and low matrix effect in drinking water and moderate effect in surface one.
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