A Novel Graphene Quantum Dot-Based mRNA Delivery Platform

: During the last decades, there has been growing interest in using therapeutic messager RNA (mRNA) together with drug delivery systems. Naked, unformulated mRNA is, however, unable to cross the cell membrane and is susceptible to degradation. Here we use graphene quantum dots (GQDs) functionalized with polyethyleneimine (PEI) as a novel mRNA delivery system. Our results show that these modified GQDs can be used to deliver intact and functional mRNA to Huh-7 hepatocarcinoma cells at low doses and, that the GQDs are not toxic, although cellular toxicity is a problem for these first-generation modified particles. Functionalized GQDs represent a potentially interesting delivery system that is easy to manufacture, stable and effective.

Automated summary

The study explores the use of graphene quantum dots functionalized with polyethyleneimine as a novel mRNA delivery system that can deliver intact and functional mRNA to hepatocarcinoma cells without toxicity. While cellular toxicity remains a concern, the functionalized GQDs show promise as a potentially interesting, easy to manufacture, stable, and effective delivery system.