Article
Biochemistry & Molecular Biology
Fernando Gisbert Roca, Luis Gil Santos, Manuel Mata Roig, Lara Milian Medina, Cristina Martinez-Ramos, Manuel Monleon Pradas
Summary: This study presents a novel multimodular nerve guidance conduit for the treatment of large nerve damages. The conduit consists of aligned PLA microfibers and HA conduits that retain pre-seeded auxiliary cells. The results demonstrate that this multimodular approach is effective for promoting nerve regeneration and provides new surgical solutions in this field.
Article
Cell Biology
Jie Yang, Chia-Chen Hsu, Ting-Ting Cao, Hua Ye, Jing Chen, Yun-Qing Li
Summary: A hyaluronic acid granular hydrogel was found to promote neuronal and astrocyte colony formation and axonal extension in vitro, mimicking an extracellular matrix structure for neural regeneration. Transplantation of the hydrogel nerve guidance conduit successfully repaired a 10-mm long sciatic nerve gap, showing similar regeneration of axons and myelin sheath, as well as recovery of electrophysiological and motor functions. The conduit outperformed bulk hydrogel or silicone tube transplant. These findings suggest that tissue-engineered nerve conduits with hyaluronic acid granular hydrogels effectively promote the morphological and functional recovery of injured peripheral nerves.
NEURAL REGENERATION RESEARCH
(2023)
Article
Engineering, Biomedical
Binghui Jin, Yunru Yu, Xiangxiang Chen, Yanhong Yang, Yushan Xiong, Young Jun Im, Yuanjin Zhao, Jian Xiao
Summary: The study proposes a novel microtube for sciatic nerve regeneration using gradient decellularized porcine sciatic nerve extracellular matrix hydrogel (pDScNM-gel). The pDScNM is shown to enhance cell proliferation and migration, improve axon growth, and these effects can also be achieved through coculture in a gradient pDScNM microtube. In vivo sciatic nerve regeneration and functional recovery are demonstrated by assembling the gradient pDScNM microtubes with a medical silicon tube.
BIOACTIVE MATERIALS
(2023)
Review
Biochemistry & Molecular Biology
Simeon C. Daeschler, Konstantin Feinberg, Leila Harhaus, Ulrich Kneser, Tessa Gordon, Gregory H. Borschel, Aleksey Zaitsev
Summary: Peripheral nerve injuries have significant impacts on individuals and society, resulting in functional impairments, lengthy rehabilitation, and socioeconomic burdens. Tacrolimus, a potent immunosuppressive drug with neuroregenerative properties, shows promise in accelerating nerve fiber regeneration. This review explores the therapeutic potential of tacrolimus through investigating its mechanisms of action and discussing practical barriers for clinical translation, providing insights into its translational perspectives as an adjunct therapy for nerve regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Multidisciplinary
Haixing Xu, Yi Yu, Lingxi Zhang, Furong Zheng, Yixia Yin, Yuxiang Gao, Kebi Li, Jingyi Xu, Jing Wen, Hui Chen, Zhixin Lei, Zhijun Huang, Dan Yu, Nan Jiang, Peihu Xu
Summary: The study investigates the repair of injured peripheral nerves in rats using PDLLA/beta-TCP nerve conduits laden with CS-HA/NGF hydrogels, showing that the hydrogels promote adhesion, spreading, and differentiation of neuronal cells while sustaining NGF release. In vivo studies demonstrate a significant positive effect on axon regeneration and myelination compared to control groups, indicating the potential of CS-HA/NGF injectable hydrogel in promoting nerve regeneration in neural tissue engineering.
COMPOSITES PART B-ENGINEERING
(2022)
Article
Engineering, Biomedical
Jiaqing Chen, Jiabei Yang, Li Wang, Xuewei Zhang, Boon Chin Heng, Dong-An Wang, Zigang Ge
Summary: The study developed a novel hydrogel, AHAMA hydrogel, with excellent adhesive strength and stability, capable of enhancing proliferation and migration of bone marrow stem cells. In experiments, AHAMA hydrogel demonstrated significant potential in promoting cartilage regeneration in a rat osteochondral defect model.
BIOACTIVE MATERIALS
(2021)
Article
Cell & Tissue Engineering
Lauren J. Pruett, Hannah L. Kenny, William M. Swift, Katarina J. Catallo, Zoe R. Apsel, Lisa S. Salopek, Philip O. Scumpia, Patrick S. Cottler, Donald R. Griffin, James J. Daniero
Summary: Biomaterial-enabled de novo formation of non-fibrotic tissue in situ is important for physicians. In the case of glottic insufficiency, a debilitating laryngeal disorder, a novel microporous annealed particle (MAP) scaffold successfully provided persistent vocal fold augmentation in rabbits, resulting in tissue de novo formation that matched the mixture of fibrotic and non-fibrotic collagens of the contralateral vocal tissue. This study demonstrates improved outcomes compared to the current clinical standard.
NPJ REGENERATIVE MEDICINE
(2023)
Article
Engineering, Biomedical
Shuai Wei, Qian Hu, Jianxiong Ma, Xiu Dai, Yu Sun, Gonghai Han, Haoye Meng, Wenjing Xu, Lei Zhang, Xinlong Ma, Jiang Peng, Yu Wang
Summary: Compared to conventional artificial nerve guide conduits, acellular nerve grafts derived from natural nerves have natural advantages. In this study, a composite technology based on supercritical carbon dioxide was used to process the peripheral nerve of a large mammal and obtain acellular nerve xenografts. The extracted grafts showed improved cell adhesion and proliferation, reduced inflammatory response, and promoted nerve regeneration.
BIOACTIVE MATERIALS
(2022)
Article
Cell & Tissue Engineering
Maki Ando, Ryosuke Ikeguchi, Tomoki Aoyama, Mai Tanaka, Takashi Noguchi, Yudai Miyazaki, Shizuka Akieda, Koichi Nakayama, Shuichi Matsuda
Summary: The study compared the long-term safety and nerve regeneration of Bio3D conduit with artificial nerve conduit and autologous nerve transplantation in treating rat sciatic nerve defect. Results showed that the Bio3D group exhibited significantly better nerve regeneration compared to the silicon group, and there was no significant difference between the Bio3D group and the nerve autograft group in all endpoints.
CELL TRANSPLANTATION
(2021)
Article
Agriculture, Dairy & Animal Science
Robert Trujanovic, Helene Rohrbach
Summary: Analgesia plays an important role in peri-operative protocols for both humans and animals. Ultrasound-guided nerve blocks have been developed to improve the effectiveness of analgesic drugs. In rabbits, a new technique for block of the sciatic and femoral nerves has been successfully tested on cadavers and may be useful in hind limb surgery. Incorporating regional anesthesia can reduce the requirement for systemic drugs and minimize side effects.
Article
Biochemistry & Molecular Biology
Liangfu Jiang, Xingyu Ouyang, Dupiao Zhang, Gang Wang, Zhe Zhang, Wei Wang, Hede Yan
Summary: This study presents a Gel-Ppy modified nerve conduit with excellent biocompatibility and conductivity, and evaluates its performance in promoting nerve regeneration in vitro and in vivo. The results demonstrate that Gel-Ppy modified nerve conduit can significantly enhance Schwann cell proliferation, contribute to Schwann cell regeneration in vivo, and improve axon diameters and myelin sheath thickness, leading to the amelioration of muscle atrophy, nerve conduction, and motor function recovery. Further investigation indicates that the Gel-Ppy conduit facilitates nerve regeneration by upregulating the Rap1 pathway to induce neurite outgrowth. Therefore, Gel-Ppy modified nerve conduit can provide a favorable microenvironment for nerve regeneration and be popularized as a novel therapeutic strategy for peripheral nerve injury.
Article
Chemistry, Medicinal
Haili Xiao, Chao Wei, Huiying Liu, Zhiqiang Li, Cihua Zheng, Jun Luo
Summary: The natural drug lentinan has been found to regulate autophagy, promoting the clearance of myelin debris and enhancing nerve regeneration. The study demonstrated that lentinan activates the AMPK/mTOR signaling pathway, accelerating autophagic flux and promoting the phagocytosis of myelin debris by Schwann cells. These findings suggest that lentinan may be a cost-effective and natural treatment for peripheral nerve injury.
PHYTOTHERAPY RESEARCH
(2023)
Review
Health Care Sciences & Services
Maximilian Mayrhofer-Schmid, Tess T. Klemm, Martin Aman, Ulrich Kneser, Kyle R. Eberlin, Leila Harhaus, Arne H. Boecker
Summary: Wrapping peripheral nerves can prevent scar tissue formation and adhesions, but the optimal material and technique have yet to be determined. Further research is needed to identify the most effective nerve wrapping method for clinical practice.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Biotechnology & Applied Microbiology
Yingshuai Wang, Tao Yu, Feihu Hu
Summary: This study explores the application of miRNAs and exosomes in peripheral nerve regeneration and finds that a hypocapnia environment can enhance exosome function. By delivering miR-218 through engineered exosomes, sciatic nerve regeneration can be facilitated, and hypocapnia stimuli-responsive exosomes show better results.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Materials Science, Textiles
Mehdi Keikha, Elahe Entekhabi, Mahvash Shokrollahi, Masoumeh Haghbin Nazarpak, Zahra Hassannejad, Somaye Akbari, Alex R. Vaccaro, Vafa Rahimi-Movaghar
Summary: This study aimed to design composite electrospun scaffolds based on polylactic acid/hyaluronic acid containing different percentages of Methylprednisolone, and evaluate their performance. Results showed that composite scaffolds containing 0.5 w/v Methylprednisolone had lower cytotoxicity and higher biocompatibility.
JOURNAL OF INDUSTRIAL TEXTILES
(2022)
Article
Medicine, General & Internal
Shkelzen B. Duci, Hysni M. Arifi, Hasan R. Ahmeti, Suzana Manxhuka-Kerliu, Burim Neziri, Agon Y. Mekaj, Shpetim Lajqi, Labinot Shahini
CHINESE MEDICAL JOURNAL
(2015)
Article
Agriculture, Dairy & Animal Science
Fatgzim Latifi, Zana Baruti-Gafurri, Imer Haziri, Suzana Manxhuka-Kerliu, Labinot Shahini, Arjeta Podrimaj-Bytyqi, Arben Sinani, Sherein S. Abdelgayed
Summary: This study aimed to evaluate hormone concentrations in male dogs from different regions of Kosovo and histopathologically evaluate their testicular tissue. Testosterone, LH, and FSH levels were found to vary between regions, with different histological changes observed in the testicular tissues. Hormone analyses and histopathological examinations could be useful for fertility assessments in male dogs.
INDIAN JOURNAL OF ANIMAL RESEARCH
(2021)
Article
Medicine, General & Internal
Naim Zeka, Leonore Zogaj, Abdurrahim Gerguri, Ramush Bejiqi, Ragip Ratkoceri, Arlinda Maloku, Aferdita Mustafa, Labinot Shahini, Jeton Maxharaj
Summary: Lafora disease is a rare genetic disorder characterized by progressive myoclonus epilepsy and neurologic deterioration. We report a rare case of Lafora disease in a 16-year-old Albanian girl who presented with generalized tonic-clonic seizures and cognitive dysfunction. Blood tests, electroencephalography, and brain magnetic resonance imaging showed no abnormalities. Genetic testing confirmed a positive mutation on the NHLRC1 gene, leading to the diagnosis of Lafora disease.
JOURNAL OF MEDICAL CASE REPORTS
(2022)
Article
Medicine, General & Internal
Labinot Shahini, Slavko Gasparov, Gordana Petrusevska, Suzana Manxhuka Kerliu, Fahredin Veselaj, Fisnik Kurshumliu, Floren Kavaja
ACTA CLINICA CROATICA
(2017)
Article
Surgery
Shkelzen B. Duci, Hysni M. Arifi, Hasan R. Ahmeti, Suzana Manxhuka-Kerliu, Agon Y. Mekaj, Labinot Shahini, Fisnik Kurshumliu
EUROPEAN JOURNAL OF PLASTIC SURGERY
(2017)
