Review
Pharmacology & Pharmacy
Seoin Yang, Hyunwoo Choi, Duc Tai Nguyen, Nayoung Kim, Su Yeon Rhee, Sang Yeong Han, Hojae Lee, Insung S. Choi
Summary: Living cells have the unique ability to perform complex biochemical reactions with precision and efficiency, making them attractive for therapeutic applications. Recent progress has been made in the development of engineered therapeutic cells by chemically modifying cell surfaces at the single-cell level, allowing individual cells to possess designed therapeutic functions in a compatible manner. This review highlights the latest advances in therapeutic living cells using single-cell surface engineering, including potential applications in blood transfusion, drug delivery, cancer therapy, probiotic therapy, and tissue engineering and regenerative medicine. The review also briefly discusses strategies for functionalizing cell surfaces with biomolecules and materials, as well as the future direction of constructing active cell-in-shell nanobiohybrid systems for single-cell surface engineering.
ADVANCED THERAPEUTICS
(2023)
Article
Chemistry, Multidisciplinary
Sang Yeong Han, Gyeongwon Yun, Duc Tai Nguyen, Eunhye K. Kang, Hojae Lee, Seulbi Kim, Beom Jin Kim, Ji Hun Park, Insung S. Choi
Summary: In this study, the use of eggshell membrane hydrolysates and coffee melanoidins as materials for encapsulating cells is proposed. The resulting nanoshells demonstrate high biocompatibility, protecting the cells from harmful heavy metals and UV-B irradiation. This research provides a new option for interfacing living cells and materials.
Article
Chemistry, Multidisciplinary
Hojae Lee, Joohyouck Park, Nayoung Kim, Wongu Youn, Gyeongwon Yun, Sang Yeong Han, Duc Tai Nguyen, Insung S. Choi
Summary: This research describes a strategy for imparting living cells with extrinsic catalytic capability through nanoencapsulation, allowing cells to acquire new biochemical functions and enhancing their biological functions. This has important implications for catalytic bioempowerment of living cells.
ADVANCED MATERIALS
(2022)
Review
Food Science & Technology
Qing Fan, Xiaoqun Zeng, Zhen Wu, Yuxing Guo, Qiwei Du, Maolin Tu, Daodong Pan
Summary: Lactic acid bacteria (LAB) is protected from harsh conditions through nanoencapsulation using nanocellulose and starch nanoparticles as well as food-grade biopolymers such as polysaccharides and proteins. The formation of a stable physical transition layer delays the release of LAB in the gut, enhancing probiotic delivery stability.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Biochemistry & Molecular Biology
Fengying Cai, Yafeng Ren, Jiajia Dai, Jianmin Yang, Xianai Shi
Summary: Cell surface engineering technologies modify the cell surface to regulate cell function and behavior. This study investigates the effects of different surface engineering reactions on the behavior of HUVECs and human skin fibroblasts. The results show that these reactions have varying effects on cell viability, growth, proliferation, cell cycle, adhesion, and migration.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Materials Science, Multidisciplinary
Onur Hasturk, Maria J. Rodriguez, John J. Wheeler, Varun Venoor, Margaret J. Sobkowicz, David L. Kaplan
Summary: Mammalian cells lack a protective cell wall and are more sensitive to mechanical forces, making them vulnerable during processing. Nanocoating individual cells with silk-based artificial cell walls can protect against mechanical stress, preserving membrane integrity, cell survival, and proliferation during cell delivery and 3D bioprinting applications.
Article
Biochemical Research Methods
Florian Hausladen, Petra Kruse, Felicia Hessenberger, Thomas Stegmayer, Yu-Ting Kao, Wolf Seelert, Rosemarie Preyer, Marco Springer, Karl Stock, Rainer Wittig
Summary: A rapid and precise method for cytoplasmic molecule delivery in cell engineering is desired. This study investigated the manipulation of mammalian cells using single sub-nanosecond laser pulses, achieving high throughput, precision, and viability retention. By adjusting the position of the beam waist, cell damage and zones of cell ablation were induced, leading to uptake of membrane-impermeant fluorophores and GFP expression plasmids. Increasing the Rayleigh-length and beam waist diameter reduced the sensitivity to axial defocusing, resulting in robust molecule transfer. Serial application of single pulses over a moving cell monolayer achieved quantitative molecule transfer at rates up to 40%. These results are important for spatially and temporally controlled marker-free high throughput cell manipulation.
JOURNAL OF BIOPHOTONICS
(2023)
Article
Materials Science, Biomaterials
Jianghai Tang, Xuanjin Chen, Hang Shi, Mingming Zhang, Zhimin Zhou, Chuangnian Zhang, Tingyu Ke, Deling Kong, Chen Li
Summary: Pancreatic islet surface engineering with inulin, an FDA-approved dietary prebiotic, enhances islet viability, insulin secretion, and revascularization in vitro. In vivo studies show improved glucose control and reduced immune cell infiltration in hyperglycemic mice transplanted with inulin-coated islets.
BIOMATERIALS SCIENCE
(2023)
Article
Multidisciplinary Sciences
Jiezhou Pan, Guidong Gong, Qin Wang, Jiaojiao Shang, Yunxiang He, Chelsea Catania, Dan Birnbaum, Yifei Li, Zhijun Jia, Yaoyao Zhang, Neel S. Joshi, Junling Guo
Summary: The authors developed a polyphenol-based nanoarmor coating that protects probiotics from antibiotics and enhances their effectiveness against antibiotic-mediated diarrhea. The nanoarmor absorbs antibiotic molecules and allows the bacteria to colonize in the gastrointestinal tract, reducing diarrhea and inflammation caused by antibiotics.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Liangbo Chen, Feng Wu, Yan Pang, Dan Yan, Siyi Zhang, Fangjie Chen, Nianxuan Wu, Danni Gong, Jinyao Liu, Yao Fu, Xianqun Fan
Summary: The study demonstrates the formation of a durable and protective therapeutic nanocoating on the ocular surface by instilling biomimetic drug-loaded nanoparticles, which can specifically bind to ocular epithelium fibronectin and improve the stability of tear film. This approach shows therapeutic benefits in mouse and rabbit models of dry eye disease, indicating the potential of therapeutic nanocoating to treat eye diseases.
Review
Cell Biology
Maysam Mansouri, Martin Fussenegger
Summary: Cell therapy approaches utilizing engineered mammalian cells for on-demand production of therapeutic agents in the patient's body have advanced beyond proof-of-concept in translational medicine. Customized therapeutic cells can sense user-defined signals, process them, and respond programmably. Various methods of controlling cell behavior, including open-loop and closed-loop systems, are discussed, along with therapeutic applications of engineered cells in early disease diagnosis and treatment. Emerging technologies like digital devices also show potential for future incorporation into cell-based therapies.
Article
Materials Science, Biomaterials
Jie Li, Zhuang Ma, Anran Li, Siyuan Huang, Yufei Zhang, Yun Xue, Xianhui Song, Ye Zhang, Shihao Hong, Mo Wang, Zhongming Wu, Xinge Zhang
Summary: Microorganism adhesion and contamination of biomaterial can cause failure of biomedical devices. A self-assembled biomaterial nanocoating using acidity and photoregulated nanoparticles was developed to regulate adhesion and detachment of bacteria or cells. The coating showed reversible ability even after 8 cycles, making it a promising agent for biomedical device development, especially for medical coatings.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Nanoscience & Nanotechnology
Maria Godoy-Gallardo, Xingli Cun, Xiaoli Liu, Leticia Hosta-Rigau
Summary: Silica replicas fabricated through a live-cell template strategy can induce the differentiation of mesenchymal stem cells into specific cell lineages, and the differentiation effect depends on the cell templates. The combination of growth factors with the replicas can enhance the differentiation rate.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Engineering, Chemical
Luanying He, Yulin Chang, Junhao Zhu, Ying Bi, Wenlin An, Yiyang Dong, Jia-Hui Liu, Shihui Wang
Summary: Encapsulation of graphene oxide (GO) on yeast cells increases roughness, enhances survival time and activity, and improves resistance against pH and salt stresses, thereby extending storage time. Optimization of encapsulation layers can help prolong the storage time of yeast cells.
FRONTIERS OF CHEMICAL SCIENCE AND ENGINEERING
(2021)
Article
Pharmacology & Pharmacy
Ziyan Zhang, Qiaojuan Liu, Jizhou Tan, Xiaoxia Zhan, Ting Liu, Yuting Wang, Gen Lu, Minhao Wu, Yuanqing Zhang
Summary: This study demonstrated a novel strategy for in vitro T cell-selective protection by constructing a DNA network and utilizing a DNA nanostructure, which enhanced the resistance of T cells to external stimuli and showed potential for application in adoptive cell therapy.
ACTA PHARMACEUTICA SINICA B
(2021)
Article
Chemistry, Multidisciplinary
Sang Yeong Han, Gyeongwon Yun, Duc Tai Nguyen, Eunhye K. Kang, Hojae Lee, Seulbi Kim, Beom Jin Kim, Ji Hun Park, Insung S. Choi
Summary: In this study, the use of eggshell membrane hydrolysates and coffee melanoidins as materials for encapsulating cells is proposed. The resulting nanoshells demonstrate high biocompatibility, protecting the cells from harmful heavy metals and UV-B irradiation. This research provides a new option for interfacing living cells and materials.
Article
Chemistry, Multidisciplinary
Hojae Lee, Joohyouck Park, Nayoung Kim, Wongu Youn, Gyeongwon Yun, Sang Yeong Han, Duc Tai Nguyen, Insung S. Choi
Summary: This research describes a strategy for imparting living cells with extrinsic catalytic capability through nanoencapsulation, allowing cells to acquire new biochemical functions and enhancing their biological functions. This has important implications for catalytic bioempowerment of living cells.
ADVANCED MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Yoonho Jeong, Jihoo Kim, Yeji Kim, Insung S. Choi
Summary: This work proposes the use of I-N - A instead of the normalized form I-N + A in graph convolutional networks for deep-learning chemistry. The performance of the GCN model with D-1/2(I-N - A)D-1/2 is comparable to the vanilla GCN using (D) over tilde (-1/2)(I-N + A)(D) over tilde in various chemistry datasets. The use of I-N - A provides more chemically intuitive information on bond properties in molecules. This work emphasizes the necessity of addressing molecular-representation problems in deep-learning chemistry from unprecedented angles.
BULLETIN OF THE KOREAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Multidisciplinary
Yeji Kim, Yoonho Jeong, Jihoo Kim, Eok Kyun Lee, Won June Kim, Insung S. Choi
Summary: Most graph neural networks in deep-learning chemistry are based on the 2D representation of molecules, but our proposed model, MolNet, incorporates 3D non-bond information and bond-strength information, achieving superior performance in classification and regression tasks.
CHEMISTRY-AN ASIAN JOURNAL
(2022)
Article
Chemistry, Physical
Vijayarohini Parasuraman, Parasuraman Perumalswamy Sekar, Hojae Lee, Mahshab Sheraz, Woo Ram Lee, Tae Young Park, Seungdo Kim
Summary: This study focuses on a multi-purpose photocatalyst filter that effectively removes organic contaminants, captures fine particulate matter, and exhibits excellent antibacterial activity. The findings indicate that a PNT-coated NWPF filter is an outstanding material for indoor air purification systems.
Article
Engineering, Environmental
Mahshab Sheraz, Huyen Ngoc Ly, Van Cam Thi Le, Van Quyet Nguyen, Furqanul Hassan Naqvi, Jin Yong Park, Hojae Lee, Seungdo Kim, Woo Ram Lee, Vijayarohini Parasuraman
Summary: In this study, a cure for indoor air pollution was developed by fabricating CuBTC metal-organic framework MOF combined with TiO2 nanoparticle hybrid material and examining its air-filtering capabilities. The resulting nanofiber-based air filter exhibited high adsorption efficiency for toluene gas and demonstrated superior PM2.5 removal performance. Compared to a pure HEPA filter, the CuBTC/TiO2/PS-C nanofiber-based HEPA filter has a very high filtration rate and negligible pressure drop.
PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
(2023)
Article
Polymer Science
Sang Yeong Han, Duc Tai Nguyen, Beom Jin Kim, Nayoung Kim, Eunhye K. Kang, Ji Hun Park, Insung S. Choi
Summary: A one-step fabrication method for thin films and shells is developed using nature-derived eggshell membrane hydrolysates (ESMHs) and coffee melanoidins (CMs), which are discarded as food waste. The nature-derived materials prove biocompatible with living cells, and the method allows for the construction of cell-in-shell nanobiohybrid structures. The shells, formed on individual Lactobacillus acidophilus, effectively protect the cells in simulated gastric fluid (SGF), with further enhanced protection through Fe3+-mediated shell augmentation.
Review
Pharmacology & Pharmacy
Seoin Yang, Hyunwoo Choi, Duc Tai Nguyen, Nayoung Kim, Su Yeon Rhee, Sang Yeong Han, Hojae Lee, Insung S. Choi
Summary: Living cells have the unique ability to perform complex biochemical reactions with precision and efficiency, making them attractive for therapeutic applications. Recent progress has been made in the development of engineered therapeutic cells by chemically modifying cell surfaces at the single-cell level, allowing individual cells to possess designed therapeutic functions in a compatible manner. This review highlights the latest advances in therapeutic living cells using single-cell surface engineering, including potential applications in blood transfusion, drug delivery, cancer therapy, probiotic therapy, and tissue engineering and regenerative medicine. The review also briefly discusses strategies for functionalizing cell surfaces with biomolecules and materials, as well as the future direction of constructing active cell-in-shell nanobiohybrid systems for single-cell surface engineering.
ADVANCED THERAPEUTICS
(2023)
Article
Materials Science, Biomaterials
Jungnam Kim, Seoin Yang, Insung S. Choi
Summary: In this study, a neuron-astrocyte sandwich coculture model was established to investigate the neurotoxicity of CBD and its effects on neuronal responses. The results showed that the support of astrocytes can significantly attenuate the neurotoxic effects of CBD and reduce cellular morphological damage. Furthermore, it was found that the increase of intracellular Ca2+ concentration and depolarization of mitochondrial membrane potential induced by CBD were noticeably ameliorated by coculturing neurons with astrocytes.
Article
Multidisciplinary Sciences
Sang Yeong Han, Nayoung Kim, Gyeongwon Yun, Hojae Lee, Insung S. Choi
Summary: In this study, a biocompatible strategy for synthesizing metal hydroxide hollow inorganic spheres (MH-HISs) was developed. The method involved mixing CaCO3 particles with metal ions in water, which resulted in the formation of hollow MH-HISs through ion-exchange reactions. The MH-HISs showed excellent mechanical durability and were capable of encapsulating enzymes without loss of catalytic activities, enabling multienzymatic cascade reactions.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Joohyouck Park, Nayoung Kim, Sang Yeong Han, Su Yeon Rhee, Duc Tai Nguyen, Hojae Lee, Insung S. Choi
Summary: Nanoencapsulation of living cells within artificial shells can enhance cell capabilities and enable the acquisition of external functions. This study reports a compositional transformation process of an enzyme-embedded nanoshell, which allows the release of functional molecules without losing activities and biorecognition.
Article
Chemistry, Multidisciplinary
Adrianna N. Shy, Jiashu Xu, Beom Jin Kim, Bing Xu
Summary: This study focuses on the enzyme-instructed self-assembly (EISA) of a phosphopeptide (1P) derived from the protein crystal structures of Merlin and CRL4(DCAF-1). The results demonstrate that even with the presence of a hydrophilic peptide, 1P can form a hydrogel at a very low volume fraction. Additionally, diastereomer 2P and enantiomer 3P of 1P are able to form hydrogels via EISA at higher concentrations than 1P.
Article
Chemistry, Multidisciplinary
Jihoo Kim, Yoonho Jeong, Won June Kim, Eok Kyun Lee, Insung S. Choi
Summary: The MolNet_Equi deep-learning model proposed in this paper can accurately predict both the magnitude and direction of molecular dipole moments, showcasing its superior performance in scalar and vectorial property prediction.
CHEMISTRY-AN ASIAN JOURNAL
(2023)
