期刊
ADVANCED HEALTHCARE MATERIALS
卷 10, 期 8, 页码 -出版社
WILEY
DOI: 10.1002/adhm.202001695
关键词
artificial periosteum; biomineralization; mesenchymal stem cells; osteogenic differentiation; silk fibroin
资金
- National Natural Science Foundation of China [31800807, 81871499]
- Fundamental Research Funds for the Central Universities [2020QNA6028]
- National Postdoctoral Science Foundation of China [2018M630678, 2019T120519]
- State of Sericulture Industry Technology System [CARS-18-ZJ0501]
- Zhejiang Provincial Key Laboratory Construction Plans [2020E10025]
Using biomacromolecules as templates to control hydroxyapatite crystal nucleation and growth, an artificial periosteum is prepared by biomineralizing Antheraea pernyi fibroin (AF) membrane with prenucleated nanoclusters. This approach promotes osteogenic differentiation of MSCs, induces bone matrix protein formation, and enhances the mechanical properties of the AF membrane. The biomineralized AF membranes show good cytocompatibility, hemocompatibility, and histocompatibility in vitro and in vivo, making them a promising strategy in the field of bone tissue engineering.
The use of biomacromolecules as templates to control the nucleation and growth of hydroxyapatite crystals to prepare bioactive materials is a valuable approach in bone tissue engineering. Here, an artificial periosteum is prepared by biomineralizing Antheraea pernyi fibroin (AF) membrane with prenucleated nanoclusters, which can promote the osteogenic differentiation of mesenchymal stem cells (MSCs) and induce the formation of bone matrix protein in vivo. To achieve this, a biologically inspired prenucleated calcium and phosphorus nanocluster mineralization system is designed to nucleate and generate hydroxyapatite crystals on the surface of the AF membrane. This biomineralization process provides AF membranes with improved elastic modulus and tensile strength. Subsequently, cell viability assay, hemolysis test, and H&E staining show that the mineralized AF (MAF) membranes has good cytocompatibility, hemocompatibility, and histocompatibility in vitro and in vivo. Additionally, the MAF membranes significantly promote osteogenic differentiation of MSCs in the absence of osteogenic inducer in vitro. Experiments in vivo demonstrate that bone-related matrix proteins are highly expressed in MAF groups with or without MSCs seeded. Therefore, the use of bioinspired prenucleated nanoclusters to prepare artificial periosteum based on biomineralized AF membrane is a promising strategy in the field of bone tissue engineering.
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